AB188539

Anti-Streptolysin O (Hemolytic streptococcus) antibody

Name: Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) | Abcam
Brand: Abcam Limited
SKU: AB188539
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Streptolysin O (Hemolytic streptococcus) antibody. Suitable for IP, FuncS (Neut/Block), WB and reacts with Recombinant full length protein, Recombinant full length protein - Streptococcus dysgalactiae subsp. equisimilis samples. Cited in 4 publications.

View Alternative Names

Streptolysin O, Streptolysin O (Thiol-activated cytolysin) slo

2 Images

Supplier Data

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (AB188539)

Most of Streptolysin O in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of Streptolysin O in the culture of this strain. Condensed sample is analyzed in the second Western Blot.

The two faint bands below Streptolysin O band are partially degraded Streptolysin O.

All lanes:

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

Lane 1:

Medium only

Lane 2:

Culture supernatant of GAS strain Sa

Lane 3:

Culture supernatant of GCS strain H46A

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (AB188539)

Culture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation.

The Streptolysin O of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B and both the concentration and size of Streptolysin O in culture medium is decreased from the native one.

All lanes:

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

Lane 1:

Concentrated culture of GSA strain Sa

Lane 2:

Concentrated medium only

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

WB, IP, FuncS (Neut/Block)

applications

Specificity

ab188539 reacts with Streptolysin O of Group A, C and G origins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "Neutralization of hemolysis by Streptolysin O (SLO).", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Streptococcus dysgalactiae subsp. equisimilis": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 6 - 8.5 Preservative: 0.09% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Streptolysin O also known as Hemolytic streptococcus or SLO is a cytolytic toxin produced by some strains of group A Streptococcus. It forms pores in host cell membranes leading to cell lysis. Streptolysin O has a molecular mass of approximately 69 kDa. This protein expresses predominantly in the bacterial cell environment during infections. It is an oxygen-labile toxin which means it is inactivated by oxygen. The immune system of the host generates antibodies called anti-streptolysin O or anti-SLO to neutralize its effects.

Biological function summary

Streptolysin O disrupts cellular membranes by binding to cholesterol which results in host cell damage and death. It is not part of a multi-protein complex but interacts directly with host cell membranes. During infection this interaction causes significant damage to tissues and allows the bacteria to invade and spread rapidly. Streptolysin O contributes significantly to the pathogenicity of Streptococcus pyogenes making the bacteria highly virulent.

Pathways

Streptolysin O influences host immune response and contributes to inflammation. During infection it activates the NF-kB signaling pathway which plays a role in immune responses and inflammation. This toxin also interacts with other proteins such as leukotoxins to enhance the virulence of the bacterial infection. The pathway activation can lead to increased production of pro-inflammatory cytokines which are part of the body's defense mechanism.

Streptolysin O is implicated in diseases like strep throat and rheumatic fever. The immune response to Streptolysin O leads to the production of anti-streptolysin O antibodies which are often measured to diagnose recent streptococcal infections. Additionally the dysregulation caused by Streptolysin O in host pathways can contribute to post-infectious complications like acute glomerulonephritis with connections to other streptococcal surface proteins such as M protein which also play roles in host-pathogen interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Streptolysin O (Hemolytic streptococcus)

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6230 PubMed37802980

2023

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Nozawa,Hirotaka Toh,Junpei Iibushi,Kohei Kogai,Atsuko Minowa-Nozawa,Junko Satoh,Shinji Ito,Kazunori Murase,Ichiro Nakagawa

Communications biology 6:124 PubMed36721030

2023

Structural basis underlying the synergism of NADase and SLO during group A Streptococcus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Jiun Tsai,Yi-Hsin Lai,Yong-An Shi,Michal Hammel,Anthony P Duff,Andrew E Whitten,Karyn L Wilde,Chun-Ming Wu,Robert Knott,U-Ser Jeng,Chia-Yu Kang,Chih-Yu Hsu,Jian-Li Wu,Pei-Jane Tsai,Chuan Chiang-Ni,Jiunn-Jong Wu,Yee-Shin Lin,Ching-Chuan Liu,Toshiya Senda,Shuying Wang

Frontiers in immunology 11:507092 PubMed33363530

2020

Human Serum Albumin Binds Streptolysin O (SLO) Toxin Produced by Group A and Inhibits Its Cytotoxic and Hemolytic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Gian Marco Vita,Giovanna De Simone,Loris Leboffe,Francesca Montagnani,Davide Mariotti,Stefano Di Bella,Roberto Luzzati,Andrea Gori,Paolo Ascenzi,Alessandra di Masi

mBio 8: PubMed28720729

2017

Inhibition of Inflammasome-Dependent Interleukin 1β Production by Streptococcal NAD-Glycohydrolase: Evidence for Extracellular Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Dóra Hancz,Elsa Westerlund,Benedicte Bastiat-Sempe,Onkar Sharma,Christine Valfridsson,Lena Meyer,John F Love,Maghnus O'Seaghdha,Michael R Wessels,Jenny J Persson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Streptolysin O (Hemolytic streptococcus) antibody

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (AB188539)

Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (AB188539)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

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