Rabbit Recombinant Monoclonal Striatin 4 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--western-blot-img111320.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)")
![Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--immunoprecipitation-img53255.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)")
![Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--flow-cytometry-intracellular-img78783.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
Alternative names
Striatin-4, Zinedin, ZIN, STRN4
Recommended products
Rabbit Recombinant Monoclonal Striatin 4 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Striatin-4, Zinedin, ZIN, STRN4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11801
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249940 is the carrier-free version of Anti-Striatin 4 antibody [EPR11801] ab177155.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Striatin 4 also known as SG2NA or STRN4 is a scaffold protein with a molecular mass of approximately 100 kDa. It is part of the striatin family and plays an important role in the organization of multiprotein complexes. The protein is expressed in several tissues including the brain where it influences signaling pathways. Striatin 4 interacts with calmodulin and phosphatases facilitating communication and modification of signaling proteins.
- Biological function summary
Striatin 4 facilitates interactions within the striatin family interactosome and contributes to the modulation of calcium signaling through its role in calcium/calmodulin-dependent processes. As a regulatory component it is essential within protein complexes that have wide implications in cellular signaling. The protein supports roles in cell growth apoptosis and vascular function due to its ability to bring together various signaling proteins and enzymes impacting cellular behavior.
- Pathways
Researchers have identified Striatin 4 as a participant in important pathways that include the protein phosphatase 2A (PP2A) pathway and the calcium signaling pathway. Its interactions with PP2A-related proteins suggest it may serve to regulate protein dephosphorylation events thereby affecting many downstream signaling cascades. Through its participation in calcium signaling Striatin 4 works in conjunction with calcium-binding proteins influencing various physiological processes such as muscle contraction and neurotransmitter release.
- Associated diseases and disorders
Studies have linked Striatin 4 to conditions such as Alzheimer's disease and cardiovascular disorders where its role in signaling pathways may contribute to pathological changes. Its association with proteins like tau in Alzheimer's reflects its potential influence on neurodegenerative processes. Additionally the interaction of Striatin 4 with vascular-related proteins could implicate it in the progression of cardiovascular conditions making it a target of interest for disease understanding and therapeutic development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--western-blot-img111320.jpg "Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)")
Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)
This data was developed using Anti-Striatin 4 antibody [EPR11801] ab177155, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Striatin 4 antibody [EPR11801] (Anti-Striatin 4 antibody [EPR11801] ab177155) at 1/1000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: U87-MG cell lysate at 10 µg
Lane 4: Human cerebellum tissue lysate at 10 µg
Lane 5: Human fetal lung tissue lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
![Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--immunoprecipitation-img53255.jpg "Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)")
Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)
This data was developed using Anti-Striatin 4 antibody [EPR11801] ab177155, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate, immunoprecipitated using Anti-Striatin 4 antibody [EPR11801] ab177155 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] (Anti-Striatin 4 antibody [EPR11801] ab177155)
Predicted band size: 80 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--immunocytochemistry-immunofluorescence-img14220.gif "Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)")
Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)
This data was developed using Anti-Striatin 4 antibody [EPR11801] ab177155, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U87-MG cells labeling Striatin 4 with Anti-Striatin 4 antibody [EPR11801] ab177155 at 1/50 dilution.
![Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940), expandable thumbnail](https://content.abcam.com/products/images/striatin-4-antibody-epr11801-bsa-and-azide-free-ab249940--flow-cytometry-intracellular-img78783.jpg "Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)")
Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (ab249940)
This data was developed using Anti-Striatin 4 antibody [EPR11801] ab177155, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized K562 cells labeling Striatin 4 with Anti-Striatin 4 antibody [EPR11801] ab177155 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
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