AB62031

Anti-Stromal interaction molecule 1 antibody

Name: Anti-Stromal interaction molecule 1 antibody (ab62031) | Abcam
Brand: Abcam Limited
SKU: AB62031
Price: 525 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(10 Publications)

Rabbit Polyclonal Stromal interaction molecule 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human STIM1.

View Alternative Names

GOK, STIM1, Stromal interaction molecule 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (AB62031)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (AB62031)

Immunofluorescence of Stromal interaction molecule 1 in mouse spleen cells with ab62031 at 20 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (AB62031)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (AB62031)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling Stromal interaction molecule 1 with Anti-Stromal interaction molecule 1 antibody (ab62031) at 5μg/ml.

Unknown

Western blot - Anti-Stromal interaction molecule 1 antibody (AB62031)

Lane 1:

Western blot - Anti-Stromal interaction molecule 1 antibody (ab62031) at 1 µg/mL

Lane 2:

Western blot - Anti-Stromal interaction molecule 1 antibody (ab62031) at 2 µg/mL

All lanes:

Mouse thymus tissue lysate at 15 µg

Predicted band size: 77 kDa

Observed band size: 116 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human STIM1. The exact immunogen used to generate this antibody is proprietary information.

Q13586

Specificity

ab62031 detects the larger isoform of Stromal interaction molecule 1 only. It is predicted to have no cross-reactivity with Stromal interaction molecule 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stromal interaction molecule 1 also known as STIM1 is a single-pass transmembrane protein primarily involved in the regulation of calcium ion entry into cells. It acts as a sensor of calcium levels inside the endoplasmic reticulum. STIM1 is about 90 kDa in mass and expresses in various tissues including the immune system skeletal muscle and vascular smooth muscle. The protein anchors in the membrane of the endoplasmic reticulum where it responds to the depletion of calcium by communicating with plasma membrane channels to activate calcium influx.

Biological function summary

STIM1 plays a critical role in the store-operated calcium entry (SOCE) mechanism a significant process in cellular calcium signaling. STIM1 senses calcium concentration within the endoplasmic reticulum and upon depletion aggregates and migrates to the plasma membrane to interact with Orai1 part of the calcium release-activated calcium (CRAC) channel complex. This interaction opens the channel allowing calcium ions to flow into the cytosol which is necessary for various cellular functions such as gene expression and cell proliferation.

Pathways

STIM1 functions integrally in the calcium signaling pathway a system central to numerous physiological processes. Here STIM1 ensures appropriate calcium ion concentrations facilitate activation of various transcription factors and enzymes. It closely associates with Orai1 triggering the CRAC channel activation in response to the endoplasmic reticulum calcium levels. Additionally STIM1 interacts with other signaling molecules like protein kinase C coordinating between different pathways to maintain cellular homeostasis.

STIM1 dysfunction links to conditions such as immunodeficiency and muscle disorders. Mutations in STIM1 can lead to combined immunodeficiency characterized by impaired immune responses due to faulty calcium influx in lymphocytes. Additionally aberrations in STIM1 activity associate with tubular aggregate myopathy a muscle disorder where abnormal calcium handling in muscle cells leads to muscle weakness and pain. In these diseases the interaction between STIM1 and Orai1 is often disrupted highlighting their roles in maintaining physiological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 15866891, PubMed : 16005298, PubMed : 16208375, PubMed : 16537481, PubMed : 16733527, PubMed : 16766533, PubMed : 16807233, PubMed : 18854159, PubMed : 19182790, PubMed : 19249086, PubMed : 19622606, PubMed : 19706554, PubMed : 22464749, PubMed : 24069340, PubMed : 24351972, PubMed : 24591628, PubMed : 25326555, PubMed : 26322679, PubMed : 28219928, PubMed : 32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed : 16208375, PubMed : 16537481, PubMed : 32415068). Involved in enamel formation (PubMed : 24621671).

See full target information STIM1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112719 PubMed40546935

2025

Taurine ameliorates blood pressure and vascular function in patients with type 2 diabetes: Randomized, double-blind, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Yingsha Li,Qianran Wang,Yi Liu,Qiang Li,Weijie Xia,Shaoyang Lin,Qian Yang,Alexandra Scholze,Zhigang Zhao,Hongbo He,Hexuan Zhang,Zhencheng Yan,Martin Tepel,Huaming Mu,Zhiming Zhu,Daoyan Liu

Cellular and molecular life sciences : CMLS 81:423 PubMed39367914

2024

Eldecalcitol ameliorates diabetic osteoporosis and glucolipid metabolic disorder by promoting Treg cell differentiation through SOCE.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Jiang,Ruihan Gao,Qiaohui Ying,Xiaolin Li,Yaling Dai,Aimei Song,Hongrui Liu,Tomoka Hasegawa,Minqi Li

Cell biology and toxicology 40:13 PubMed38347241

2024

Nupr1-mediated vascular smooth muscle cell phenotype transformation involved in methamphetamine induces pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Dan Guo,Zhen-Zhen Xu,Jia-Shun Liao,Xiao-Ting Li,Ke Duan,Shi-You Chen,Wei-Bing Xie

Molecules (Basel, Switzerland) 28: PubMed36677874

2023

The miRNA-185-5p/STIM1 Axis Regulates the Invasiveness of Nasopharyngeal Carcinoma Cell Lines by Modulating EGFR Activation-Stimulated Switch from E- to N-Cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Luo,Jiaxiang Ye,Yayan Deng,Yujuan Huang,Xue Liu,Qian He,Yong Chen,Qiuyun Li,Yan Lin,Rong Liang,Yongqiang Li,Jiazhang Wei,Jinyan Zhang

PloS one 16:e0254710 PubMed34324543

2021

Aging increases senescence, calcium signaling, and extracellular matrix deposition in human airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Wicher,Benjamin B Roos,Jacob J Teske,Yun Hua Fang,Christina Pabelick,Y S Prakash

Cancer letters 478:122-132 PubMed32165272

2020

EB virus promotes metastatic potential by boosting STIM1-dependent Ca signaling in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiazhang Wei,Jiaxiang Ye,Yue Luo,Jingjin Weng,Qian He,Fei Liu,Min Li,Yan Lin,Yongqiang Li,Zhe Zhang,Shenhong Qu,Jinyan Zhang

Cell cycle (Georgetown, Tex.) 18:682-695 PubMed30784343

2019

miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Fang,Yanfei Li,Yingbiao Wu,Zhongping Ning,Xuejun Wang,Xinming Li

Cellular physiology and biochemistry : internation 36:1101-15 PubMed26113109

2015

Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Venkatachalem Sathish,Michelle R Freeman,Emma Long,Michael A Thompson,Christina M Pabelick,Y S Prakash

Biochimica et biophysica acta 1833:2953-2960 PubMed23899746

2013

TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Pawan K Vohra,Michael A Thompson,Venkatachalem Sathish,Alexander Kiel,Calvin Jerde,Christina M Pabelick,Brij B Singh,Y S Prakash

PloS one 7:e44343 PubMed22952960

2012

Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle.

Applications

Species

Unspecified reactive species

Amard J Abcejo,Venkatachalem Sathish,Dan F Smelter,Bharathi Aravamudan,Michael A Thompson,William R Hartman,Christina M Pabelick,Y S Prakash

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Stromal interaction molecule 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (AB62031)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (AB62031)

Western blot - Anti-Stromal interaction molecule 1 antibody (AB62031)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise