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AB108994

Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(1 Review)

|

(25 Publications)

Rabbit Recombinant Monoclonal Stromal interaction molecule 1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 25 publications.

View Alternative Names

GOK, STIM1, Stromal interaction molecule 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)

ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)
  • WB

Lab

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

K562 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)
  • WB

Unknown

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)

All lanes:

Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

Lane 1:

SH SY5Y cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

LnCaP cell lysate at 10 µg

Predicted band size: 77 kDa

false

  • Carrier free

    Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free

  • 660 APC

    APC Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Stromal interaction molecule 1 antibody [EPR3414]

  • 578 PE

    PE Anti-Stromal interaction molecule 1 antibody [EPR3414]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3414

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stromal interaction molecule 1 also known as STIM1 is a single-pass transmembrane protein primarily involved in the regulation of calcium ion entry into cells. It acts as a sensor of calcium levels inside the endoplasmic reticulum. STIM1 is about 90 kDa in mass and expresses in various tissues including the immune system skeletal muscle and vascular smooth muscle. The protein anchors in the membrane of the endoplasmic reticulum where it responds to the depletion of calcium by communicating with plasma membrane channels to activate calcium influx.
Biological function summary

STIM1 plays a critical role in the store-operated calcium entry (SOCE) mechanism a significant process in cellular calcium signaling. STIM1 senses calcium concentration within the endoplasmic reticulum and upon depletion aggregates and migrates to the plasma membrane to interact with Orai1 part of the calcium release-activated calcium (CRAC) channel complex. This interaction opens the channel allowing calcium ions to flow into the cytosol which is necessary for various cellular functions such as gene expression and cell proliferation.

Pathways

STIM1 functions integrally in the calcium signaling pathway a system central to numerous physiological processes. Here STIM1 ensures appropriate calcium ion concentrations facilitate activation of various transcription factors and enzymes. It closely associates with Orai1 triggering the CRAC channel activation in response to the endoplasmic reticulum calcium levels. Additionally STIM1 interacts with other signaling molecules like protein kinase C coordinating between different pathways to maintain cellular homeostasis.

STIM1 dysfunction links to conditions such as immunodeficiency and muscle disorders. Mutations in STIM1 can lead to combined immunodeficiency characterized by impaired immune responses due to faulty calcium influx in lymphocytes. Additionally aberrations in STIM1 activity associate with tubular aggregate myopathy a muscle disorder where abnormal calcium handling in muscle cells leads to muscle weakness and pain. In these diseases the interaction between STIM1 and Orai1 is often disrupted highlighting their roles in maintaining physiological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 15866891, PubMed : 16005298, PubMed : 16208375, PubMed : 16537481, PubMed : 16733527, PubMed : 16766533, PubMed : 16807233, PubMed : 18854159, PubMed : 19182790, PubMed : 19249086, PubMed : 19622606, PubMed : 19706554, PubMed : 22464749, PubMed : 24069340, PubMed : 24351972, PubMed : 24591628, PubMed : 25326555, PubMed : 26322679, PubMed : 28219928, PubMed : 32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed : 16208375, PubMed : 16537481, PubMed : 32415068). Involved in enamel formation (PubMed : 24621671).
See full target information STIM1
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:4042-4054 PubMed39384697

2024

Low-frequency rTMS Plays a Neuroprotective role in Pilocarpine-induced Status Epilepticus Rat Models Through the AMPAR GluA1-STIM-Ca Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Qin Che,Zhen-Zhen Qu,Zhuo-Feng Mao,Qi Qiao,Kai-Ping Zhou,Li-Jing Jia,Wei-Ping Wang

Oncology letters 28:339 PubMed38855503

2024

3,3'-Diindolylmethane inhibits the proliferation of esophageal squamous cell carcinoma cells via downregulation of STIM1.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyi Xiong,Yining Tang,Feng Li,Yang Ye,Xiaoran Li,Jinxing Lin,Sunxian Dai

Experimental and therapeutic medicine 26:575 PubMed38023363

2023

IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia by alleviating endoplasmic reticulum stress and the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Qigang Dai,Ning Su,Zhihui Liu,Jinxing Hu

Cardiovascular diagnosis and therapy 13:441-452 PubMed37405015

2023

Sarsasapogenin blocks ox-LDL-stimulated vascular smooth muscle cell proliferation, migration, and invasion through suppressing STIM1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Zhai,Hongqin Liu,Baoling Shang,Xu Zou

The Journal of biological chemistry 298:102681 PubMed36356899

2022

MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Tanwar,Akshay Sharma,Suman Saurav, Shyamveer,Nidhi Jatana,Rajender K Motiani

Frontiers in immunology 13:835936 PubMed35663942

2022

Intracellular Ca Signaling and Calcium Release-Activated Calcium Modulator ORAI1 Are Associated With CD4 T Lymphocytes in Dairy Cows.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Bingbing Zhang,Yufeng Yin,Jianan Wen,Jingjing Wang,Yuxin He,Qianming Jiang,Juan J Loor,Shuang Wang,Wei Yang,Chuang Xu

PloS one 17:e0264146 PubMed35213584

2022

Quantification of the calcium signaling deficit in muscles devoid of triadin.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Manno,Eshwar Tammineni,Lourdes Figueroa,Isabelle Marty,Eduardo Ríos

International journal of molecular sciences 23: PubMed35008585

2021

STIM1 Controls the Focal Adhesion Dynamics and Cell Migration by Regulating SOCE in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shan Lin,Yi-Hsin Lin,MyHang Nguyen Thi,Shih-Chuan Hsiao,Wen-Tai Chiu

Cells 10: PubMed34685684

2021

Apoptotic Cells Trigger Calcium Entry in Phagocytes by Inducing the Orai1-STIM1 Association.

Applications

Unspecified application

Species

Unspecified reactive species

Deokhwan Kim,Hyunji Moon,Hyeokjin Cho,Chanhyuk Min,Byeongjin Moon,Susumin Yang,Juyeon Lee,Sang-Ah Lee,Hyunjin Park,Dae-Hee Lee,Dongtak Jeong,Gwangrog Lee,Daeho Park

Frontiers in pharmacology 12:622774 PubMed34079454

2021

Ang II Promotes Cardiac Autophagy and Hypertrophy via Orai1/STIM1.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Bo Zheng,Wen-Cong Gao,Mingxu Xie,Zhichao Li,Xin Ma,Wencong Song,Dan Luo,Yongxiang Huang,Jichen Yang,Peng Zhang,Yu Huang,Weimin Yang,Xiaoqiang Yao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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