Rabbit Recombinant Monoclonal Stromal interaction molecule 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (AB239938), expandable thumbnail](https://content.abcam.com/products/images/stromal-interaction-molecule-1-antibody-epr3414-bsa-and-azide-free-ab239938--western-blot-img140601.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (AB239938)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19182790, PubMed:19249086, PubMed:19622606, PubMed:19706554, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:25326555, PubMed:26322679, PubMed:28219928, PubMed:32415068). Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry (PubMed:16208375, PubMed:16537481, PubMed:32415068). Involved in enamel formation (PubMed:24621671).
Alternative names
GOK, STIM1, Stromal interaction molecule 1
Recommended products
Rabbit Recombinant Monoclonal Stromal interaction molecule 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3414
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239938 is the carrier-free version of Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Stromal interaction molecule 1 also known as STIM1 is a single-pass transmembrane protein primarily involved in the regulation of calcium ion entry into cells. It acts as a sensor of calcium levels inside the endoplasmic reticulum. STIM1 is about 90 kDa in mass and expresses in various tissues including the immune system skeletal muscle and vascular smooth muscle. The protein anchors in the membrane of the endoplasmic reticulum where it responds to the depletion of calcium by communicating with plasma membrane channels to activate calcium influx.
- Biological function summary
STIM1 plays a critical role in the store-operated calcium entry (SOCE) mechanism a significant process in cellular calcium signaling. STIM1 senses calcium concentration within the endoplasmic reticulum and upon depletion aggregates and migrates to the plasma membrane to interact with Orai1 part of the calcium release-activated calcium (CRAC) channel complex. This interaction opens the channel allowing calcium ions to flow into the cytosol which is necessary for various cellular functions such as gene expression and cell proliferation.
- Pathways
STIM1 functions integrally in the calcium signaling pathway a system central to numerous physiological processes. Here STIM1 ensures appropriate calcium ion concentrations facilitate activation of various transcription factors and enzymes. It closely associates with Orai1 triggering the CRAC channel activation in response to the endoplasmic reticulum calcium levels. Additionally STIM1 interacts with other signaling molecules like protein kinase C coordinating between different pathways to maintain cellular homeostasis.
- Associated diseases and disorders
STIM1 dysfunction links to conditions such as immunodeficiency and muscle disorders. Mutations in STIM1 can lead to combined immunodeficiency characterized by impaired immune responses due to faulty calcium influx in lymphocytes. Additionally aberrations in STIM1 activity associate with tubular aggregate myopathy a muscle disorder where abnormal calcium handling in muscle cells leads to muscle weakness and pain. In these diseases the interaction between STIM1 and Orai1 is often disrupted highlighting their roles in maintaining physiological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938), expandable thumbnail](https://content.abcam.com/products/images/stromal-interaction-molecule-1-antibody-epr3414-bsa-and-azide-free-ab239938--western-blot-img140601.jpg "Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938)")
Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994 observed at 77 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994).
All lanes: Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate at 20 µg
Lane 3: K562 whole cell lysate at 20 µg
Lane 4: HeLa whole cell lysate at 20 µg
Predicted band size: 77 kDa
Observed band size: 77 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938), expandable thumbnail](https://content.abcam.com/products/images/stromal-interaction-molecule-1-antibody-epr3414-bsa-and-azide-free-ab239938--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139888.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938)
Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stromal interaction molecule 1 antibody [EPR3414] ab108994).
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