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Rabbit Polyclonal Strumpellin antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WASHC5 aa 900-1150.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (AB126109), expandable thumbnail
  • Western blot - Anti-Strumpellin antibody (AB126109), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human WASHC5 aa 900-1150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12768

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). May be involved in axonal outgrowth. Involved in cellular localization of ADRB2 (PubMed:23085491). Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (PubMed:23676666).

Alternative names

Recommended products

Rabbit Polyclonal Strumpellin antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WASHC5 aa 900-1150.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human WASHC5 aa 900-1150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12768
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Strumpellin also known as KIAA0196 is an important component of the WASH complex. With a molecular weight of approximately 136 kDa Strumpellin plays a mechanical role in cellular operations by interacting with actin filaments assisting in the control of actin polymerization. This protein is expressed in various tissues including the nervous system and muscle tissues highlighting its involvement in diverse cellular mechanisms. Its presence in these areas suggests an essential function in maintaining normal cellular activities.

Biological function summary

Strumpellin acts as a regulator within the WASH complex which is vital for actin-related trafficking and endosomal sorting. The WASH complex where Strumpellin is a vital part helps modulate the cytoskeleton by stimulating actin nucleation. This regulation supports processes such as vesicle trafficking and maintenance of cell morphology. Without proper function of Strumpellin and the WASH complex cellular transport mechanisms may become disrupted affecting delivery and recycling of membrane components.

Pathways

Strumpellin's function is significant in endocytic trafficking and autophagy pathways. Within these pathways Strumpellin collaborates with other proteins such as F-actin to maintain efficient recycling and degradation of cellular components. Strumpellin's interaction with actin-related pathways ensures cellular homeostasis by coordinating the dynamic restructuring of the cytoskeleton and influencing processes like cell division and migration.

Associated diseases and disorders

Strumpellin has been linked to conditions such as hereditary spastic paraplegia and neurodegenerative disorders. Mutations in the Strumpellin gene disrupt normal function and contribute to these diseases. Its interaction with proteins like Spastin highlights a significant connection as both proteins are implicated in maintaining neuronal health. Understanding Strumpellin's role offers insight into the development of therapeutic strategies for related disorders.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (ab126109), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (ab126109)

    ab126109, at a 1/500 dilution, staining Strumpellin in paraffin-embedded Human gastric carcnoma tissue by immunohistochemistry.

  • Western blot - Anti-Strumpellin antibody (ab126109), expandable thumbnail

    Western blot - Anti-Strumpellin antibody (ab126109)

    5% SDS Page

    All lanes: Western blot - Anti-Strumpellin antibody (ab126109) at 1/500 dilution

    All lanes: HeLa cell lysate at 30 µg

    Predicted band size: 134 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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