Rabbit Polyclonal Strumpellin antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WASHC5 aa 900-1150.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WASHC5 aa 900-1150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12768
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). May be involved in axonal outgrowth. Involved in cellular localization of ADRB2 (PubMed:23085491). Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (PubMed:23676666).
Alternative names
WASH complex subunit 5, Strumpellin, WASH complex subunit strumpellin, KIAA0196, WASHC5
Recommended products
Rabbit Polyclonal Strumpellin antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WASHC5 aa 900-1150.
Alternative names
WASH complex subunit 5, Strumpellin, WASH complex subunit strumpellin, KIAA0196, WASHC5
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WASHC5 aa 900-1150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12768
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Strumpellin also known as KIAA0196 is an important component of the WASH complex. With a molecular weight of approximately 136 kDa Strumpellin plays a mechanical role in cellular operations by interacting with actin filaments assisting in the control of actin polymerization. This protein is expressed in various tissues including the nervous system and muscle tissues highlighting its involvement in diverse cellular mechanisms. Its presence in these areas suggests an essential function in maintaining normal cellular activities.
- Biological function summary
Strumpellin acts as a regulator within the WASH complex which is vital for actin-related trafficking and endosomal sorting. The WASH complex where Strumpellin is a vital part helps modulate the cytoskeleton by stimulating actin nucleation. This regulation supports processes such as vesicle trafficking and maintenance of cell morphology. Without proper function of Strumpellin and the WASH complex cellular transport mechanisms may become disrupted affecting delivery and recycling of membrane components.
- Pathways
Strumpellin's function is significant in endocytic trafficking and autophagy pathways. Within these pathways Strumpellin collaborates with other proteins such as F-actin to maintain efficient recycling and degradation of cellular components. Strumpellin's interaction with actin-related pathways ensures cellular homeostasis by coordinating the dynamic restructuring of the cytoskeleton and influencing processes like cell division and migration.
- Associated diseases and disorders
Strumpellin has been linked to conditions such as hereditary spastic paraplegia and neurodegenerative disorders. Mutations in the Strumpellin gene disrupt normal function and contribute to these diseases. Its interaction with proteins like Spastin highlights a significant connection as both proteins are implicated in maintaining neuronal health. Understanding Strumpellin's role offers insight into the development of therapeutic strategies for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (ab126109)
ab126109, at a 1/500 dilution, staining Strumpellin in paraffin-embedded Human gastric carcnoma tissue by immunohistochemistry.
Western blot - Anti-Strumpellin antibody (ab126109)
5% SDS Page
All lanes: Western blot - Anti-Strumpellin antibody (ab126109) at 1/500 dilution
All lanes: HeLa cell lysate at 30 µg
Predicted band size: 134 kDa
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