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AB126109

Anti-Strumpellin antibody

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(1 Publication)

Rabbit Polyclonal Strumpellin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WASHC5 aa 900-1150.

View Alternative Names

KIAA0196, WASHC5, WASH complex subunit 5, Strumpellin, WASH complex subunit strumpellin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (AB126109)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strumpellin antibody (AB126109)

ab126109, at a 1/500 dilution, staining Strumpellin in paraffin-embedded Human gastric carcnoma tissue by immunohistochemistry.

Western blot - Anti-Strumpellin antibody (AB126109)
  • WB

Unknown

Western blot - Anti-Strumpellin antibody (AB126109)

5% SDS Page

All lanes:

Western blot - Anti-Strumpellin antibody (ab126109) at 1/500 dilution

All lanes:

HeLa cell lysate at 30 µg

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WASHC5 aa 900-1150. The exact immunogen used to generate this antibody is proprietary information.

Q12768

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Strumpellin also known as KIAA0196 is an important component of the WASH complex. With a molecular weight of approximately 136 kDa Strumpellin plays a mechanical role in cellular operations by interacting with actin filaments assisting in the control of actin polymerization. This protein is expressed in various tissues including the nervous system and muscle tissues highlighting its involvement in diverse cellular mechanisms. Its presence in these areas suggests an essential function in maintaining normal cellular activities.
Biological function summary

Strumpellin acts as a regulator within the WASH complex which is vital for actin-related trafficking and endosomal sorting. The WASH complex where Strumpellin is a vital part helps modulate the cytoskeleton by stimulating actin nucleation. This regulation supports processes such as vesicle trafficking and maintenance of cell morphology. Without proper function of Strumpellin and the WASH complex cellular transport mechanisms may become disrupted affecting delivery and recycling of membrane components.

Pathways

Strumpellin's function is significant in endocytic trafficking and autophagy pathways. Within these pathways Strumpellin collaborates with other proteins such as F-actin to maintain efficient recycling and degradation of cellular components. Strumpellin's interaction with actin-related pathways ensures cellular homeostasis by coordinating the dynamic restructuring of the cytoskeleton and influencing processes like cell division and migration.

Strumpellin has been linked to conditions such as hereditary spastic paraplegia and neurodegenerative disorders. Mutations in the Strumpellin gene disrupt normal function and contribute to these diseases. Its interaction with proteins like Spastin highlights a significant connection as both proteins are implicated in maintaining neuronal health. Understanding Strumpellin's role offers insight into the development of therapeutic strategies for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed : 19922875, PubMed : 20498093). May be involved in axonal outgrowth. Involved in cellular localization of ADRB2 (PubMed : 23085491). Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (PubMed : 23676666).
See full target information WASHC5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 12:5538-5550 PubMed33042436

2020

Oxymatrine inhibits the development of non-small cell lung cancer through miR-367-3p upregulation and target gene downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Yu,Jiewei Luo,Jiguang Zhang,Yangming Chen,Kai Chen,Jianbin Lin,Shihui Sun,Xing Lin
View all publications
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Product promise

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