Rabbit Polyclonal Sts1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ubash3b.
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Publications
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- Proceedings of the National Academy of Sciences of the United States of America 119:e21171751192022Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodolfo Ciuffa,Federico Uliana,Jonathan Mannion,Martin Mehnert,Tencho Tenev,Cathy Marulli,Ari Satanowski,Lena Maria Leone Keller,Pilar Natalia Rodilla Ramírez,Alessandro Ori,Matthias Gstaiger,Pascal Meier,Ruedi AebersoldPubMed 36179048
- The Journal of experimental medicine 218:2021Cbl-b deficiency prevents functional but not phenotypic T cell anergy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang T T Nguyen,Zhi-En Wang,Lin Shen,Andrew Schroeder,Walter Eckalbar,Arthur WeissPubMed 33974042
- Nature communications 12:712021Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi Ke et. al.PubMed 33397984
- Evidence-based complementary and alternative medic 2020:88958092020Ziyin Bushen Decoction Alleviates Perimenopausal Syndrome in Rats by Enhancing Estradiol Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBi-Xin Tang et. al.PubMed 33204296
- The Journal of biological chemistry 294:12112-121212019Hepatic steroid sulfatase critically determines estrogenic activities of conjugated equine estrogens in human cells and in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Feng et. al.PubMed 31217279
- The Journal of experimental medicine 213:399-4142016Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.
- Nature immunology 16:1253-622015K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Ubash3b. The exact immunogen used to generate this antibody is proprietary information. Database link Q8BGG7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Dog | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
Alternative names
KIAA1959, STS1, UBASH3B, Ubiquitin-associated and SH3 domain-containing protein B, Cbl-interacting protein p70, Suppressor of T-cell receptor signaling 1, T-cell ubiquitin ligand 2, Tyrosine-protein phosphatase STS1/TULA2, STS-1, TULA-2
Recommended products
Rabbit Polyclonal Sts1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ubash3b.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Ubash3b. The exact immunogen used to generate this antibody is proprietary information. Database link Q8BGG7
- Purification technique
- Affinity purification Immunogen
- Specificity
A BLAST analysis was used to suggest reactivity with this protein from mouse, human, rat and dog based on 100% homology for the immunogen sequence. Cross reactivity with Sts-1 homologues from other sources has not been determined.
- Concentration
- Purification notes
This affinity purified antibody is directed against mouse Sts-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sts1 also known as Suppressor of T-cell receptor signaling 1 is a protein with a mass of approximately 72 kDa. It functions mechanically by serving as a negative regulator in T-cell receptor signaling pathways. Sts1 contains N-terminal SH3 domains and C-terminal ubiquitin-associated domains essential for its regulatory activity. Expression of Sts1 is widespread with notable abundance in immune cells such as T-cells and B-cells. These structural features enable Sts1 to interact with various signaling molecules.
- Biological function summary
In cellular processes Sts1 plays a role in modulating immune responses. It interacts with signaling proteins including the ZAP-70 kinase which is central to T-cell receptor signaling. Sts1 forms a complex with several proteins involved in the ubiquitination process which marks proteins for degradation or alters their function. By this Sts1 influences the strength and duration of signals received by the immune cells ensuring proper immune system function.
- Pathways
Sts1 serves in the T-cell receptor signaling pathway and contributes to the modulation of the immune response. The protein is closely linked to ubiquitin-proteasome system pathways which govern protein turnover and cellular regulation. In these pathways Sts1 associates with proteins like c-Cbl which are involved in the ubiquitination and degradation of signaling intermediates.
- Associated diseases and disorders
Deficiencies or dysregulation of Sts1 are often associated with autoimmune diseases and certain lymphomas. Its interplay with proteins like c-Cbl or ZAP-70 can result in aberrant T-cell activation and uncontrolled proliferation. This abnormal signaling could lead to autoimmune disorders such as systemic lupus erythematosus or contribute to the development of B-cell lymphomas where immune regulation breaks down.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sts1 antibody (ab34781)
Immunohistochemical analysis of Formalin fixed/paraffin embedded human tonsil sections labeling Sts-1 with ab34781 at 4 μg/mL. A: Negative control, B: staining of human tonsil at 20X and C: staining of human tonsil at 40X. Sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).
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