AB34781

Anti-Sts1 antibody

Name: Anti-Sts1 antibody (ab34781) | Abcam
Brand: Abcam Limited
SKU: AB34781
Price: 515 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Sts1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ubash3b.

View Alternative Names

KIAA1959, STS1, UBASH3B, Ubiquitin-associated and SH3 domain-containing protein B, Cbl-interacting protein p70, Suppressor of T-cell receptor signaling 1, T-cell ubiquitin ligand 2, Tyrosine-protein phosphatase STS1/TULA2, STS-1, TULA-2

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sts1 antibody (AB34781)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sts1 antibody (AB34781)

Immunohistochemical analysis of Formalin fixed/paraffin embedded human tonsil sections labeling Sts-1 with ab34781 at 4 μg/mL. A : Negative control, B : staining of human tonsil at 20X and C : staining of human tonsil at 40X. Sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Ubash3b. The exact immunogen used to generate this antibody is proprietary information.

Q8BGG7

Specificity

A BLAST analysis was used to suggest reactivity with this protein from mouse, human, rat and dog based on 100% homology for the immunogen sequence. Cross reactivity with Sts-1 homologues from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity purified antibody is directed against mouse Sts-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sts1 also known as Suppressor of T-cell receptor signaling 1 is a protein with a mass of approximately 72 kDa. It functions mechanically by serving as a negative regulator in T-cell receptor signaling pathways. Sts1 contains N-terminal SH3 domains and C-terminal ubiquitin-associated domains essential for its regulatory activity. Expression of Sts1 is widespread with notable abundance in immune cells such as T-cells and B-cells. These structural features enable Sts1 to interact with various signaling molecules.

Biological function summary

In cellular processes Sts1 plays a role in modulating immune responses. It interacts with signaling proteins including the ZAP-70 kinase which is central to T-cell receptor signaling. Sts1 forms a complex with several proteins involved in the ubiquitination process which marks proteins for degradation or alters their function. By this Sts1 influences the strength and duration of signals received by the immune cells ensuring proper immune system function.

Pathways

Sts1 serves in the T-cell receptor signaling pathway and contributes to the modulation of the immune response. The protein is closely linked to ubiquitin-proteasome system pathways which govern protein turnover and cellular regulation. In these pathways Sts1 associates with proteins like c-Cbl which are involved in the ubiquitination and degradation of signaling intermediates.

Deficiencies or dysregulation of Sts1 are often associated with autoimmune diseases and certain lymphomas. Its interplay with proteins like c-Cbl or ZAP-70 can result in aberrant T-cell activation and uncontrolled proliferation. This abnormal signaling could lead to autoimmune disorders such as systemic lupus erythematosus or contribute to the development of B-cell lymphomas where immune regulation breaks down.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

See full target information UBASH3B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2117175119 PubMed36179048

2022

Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Rodolfo Ciuffa,Federico Uliana,Jonathan Mannion,Martin Mehnert,Tencho Tenev,Cathy Marulli,Ari Satanowski,Lena Maria Leone Keller,Pilar Natalia Rodilla Ramírez,Alessandro Ori,Matthias Gstaiger,Pascal Meier,Ruedi Aebersold

The Journal of experimental medicine 218: PubMed33974042

2021

Cbl-b deficiency prevents functional but not phenotypic T cell anergy.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Zhi-En Wang,Lin Shen,Andrew Schroeder,Walter Eckalbar,Arthur Weiss

Nature communications 12:71 PubMed33397984

2021

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Ke,Xiao Yuan,An He,Peiyuan Yu,Wendong Chen,Yu Shi,Tony Hunter,Peng Zou,Ruijun Tian

Evidence-based complementary and alternative medic 2020:8895809 PubMed33204296

2020

Ziyin Bushen Decoction Alleviates Perimenopausal Syndrome in Rats by Enhancing Estradiol Production.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-Xin Tang,Qing-Yi Meng,Chan Xie,Shen-Shen Zhao,Kun-Lun Wu,Fang Wang,Le-Yi Du

The Journal of biological chemistry 294:12112-12121 PubMed31217279

2019

Hepatic steroid sulfatase critically determines estrogenic activities of conjugated equine estrogens in human cells and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Feng,Yang Xie,Meishu Xu,Linhao Li,Kyle W Selcer,Patrick J Oberly,Samuel M Poloyac,Hongbing Wang,Chengjiang Li,Fengqin Dong,Chaohui Yu,Wen Xie

The Journal of experimental medicine 213:399-414 PubMed26903241

2016

Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

Applications

Species

Mouse

Hongbo Hu,Hui Wang,Yichuan Xiao,Jin Jin,Jae-Hoon Chang,Qiang Zou,Xiaoping Xie,Xuhong Cheng,Shao-Cong Sun

Nature immunology 16:1253-62 PubMed26390156

2015

K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation.

Applications

Species

Unspecified reactive species

Mingjin Yang,Taoyong Chen,Xuelian Li,Zhou Yu,Songqing Tang,Chen Wang,Yan Gu,Yanfang Liu,Sheng Xu,Weihua Li,Xuemin Zhang,Jianli Wang,Xuetao Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sts1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sts1 antibody (AB34781)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise