AB197027

Anti-Sts1 antibody [EPR16980]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Sts1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA1959, STS1, UBASH3B, Ubiquitin-associated and SH3 domain-containing protein B, Cbl-interacting protein p70, Suppressor of T-cell receptor signaling 1, T-cell ubiquitin ligand 2, Tyrosine-protein phosphatase STS1/TULA2, STS-1, TULA-2

3 Images

Flow Cytometry (Intracellular) - Anti-Sts1 antibody [EPR16980] (AB197027)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Sts1 antibody [EPR16980] (AB197027)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (AB197027)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (AB197027)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1 : ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Supplier Data

Western blot - Anti-Sts1 antibody [EPR16980] (AB197027)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 72 kDa

Observed band size: 73 kDa

false

Carrier free

Anti-Sts1 antibody [EPR16980] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16980

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/280", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sts1 also known as Suppressor of T-cell receptor signaling 1 is a protein with a mass of approximately 72 kDa. It functions mechanically by serving as a negative regulator in T-cell receptor signaling pathways. Sts1 contains N-terminal SH3 domains and C-terminal ubiquitin-associated domains essential for its regulatory activity. Expression of Sts1 is widespread with notable abundance in immune cells such as T-cells and B-cells. These structural features enable Sts1 to interact with various signaling molecules.

Biological function summary

In cellular processes Sts1 plays a role in modulating immune responses. It interacts with signaling proteins including the ZAP-70 kinase which is central to T-cell receptor signaling. Sts1 forms a complex with several proteins involved in the ubiquitination process which marks proteins for degradation or alters their function. By this Sts1 influences the strength and duration of signals received by the immune cells ensuring proper immune system function.

Pathways

Sts1 serves in the T-cell receptor signaling pathway and contributes to the modulation of the immune response. The protein is closely linked to ubiquitin-proteasome system pathways which govern protein turnover and cellular regulation. In these pathways Sts1 associates with proteins like c-Cbl which are involved in the ubiquitination and degradation of signaling intermediates.

Deficiencies or dysregulation of Sts1 are often associated with autoimmune diseases and certain lymphomas. Its interplay with proteins like c-Cbl or ZAP-70 can result in aberrant T-cell activation and uncontrolled proliferation. This abnormal signaling could lead to autoimmune disorders such as systemic lupus erythematosus or contribute to the development of B-cell lymphomas where immune regulation breaks down.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

See full target information UBASH3B

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of steroid biochemistry and molecular 193:105411 PubMed31207361

2019

Mutual regulations and breast cancer cell control by steroidogenic enzymes: Dual sex-hormone receptor modulation upon 17β-HSD7 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoye Sang,Hui Han,Tang Li,Sheng-Xiang Lin

Leukemia 31:1936-1943 PubMed28626218

2017

Molecular characterization of EZH2 mutant patients with myelodysplastic/myeloproliferative neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

J Rinke,J P Müller,M F Blaess,A Chase,M Meggendorfer,V Schäfer,N Winkelmann,C Haferlach,N C P Cross,A Hochhaus,T Ernst

Nature immunology 16:1253-62 PubMed26390156

2015

K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation.

Applications

Species

Unspecified reactive species

Mingjin Yang,Taoyong Chen,Xuelian Li,Zhou Yu,Songqing Tang,Chen Wang,Yan Gu,Yanfang Liu,Sheng Xu,Weihua Li,Xuemin Zhang,Jianli Wang,Xuetao Cao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sts1 antibody [EPR16980]

Flow Cytometry (Intracellular) - Anti-Sts1 antibody [EPR16980] (AB197027)

Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (AB197027)

Western blot - Anti-Sts1 antibody [EPR16980] (AB197027)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise