Rabbit Recombinant Monoclonal STUB1/CHIP antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 20 publications.
Images
![Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (AB134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunoprecipitation-img29401.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)")
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (AB134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img17351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)")
![Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (AB134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunocytochemistry-immunofluorescence-img129457.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (AB134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)")
![Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (AB134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--flow-cytometry-intracellular-img80372.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (AB134064)")
Publications
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- iScience 26:1077622023CHIP protects against septic acute kidney injury by inhibiting NLRP3-mediated pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Zebin Deng,Yilong Wang,Xiaoping Zheng,Lizhi Zhou,Shu Yan,Yinhuai Wang,Yingbo Dai,Yashpal S Kanwar,Fei DengPubMed 37692286
- EMBO reports 24:e557622023METTL3 protects METTL14 from STUB1-mediated degradation to maintain m A homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhan-Cheng Zeng,Qi Pan,Yu-Meng Sun,Heng-Jing Huang,Xiao-Tong Chen,Tian-Qi Chen,Bo He,Hua Ye,Shun-Xin Zhu,Ke-Jia Pu,Ke Fang,Wei Huang,Yue-Qin Chen,Wen-Tao WangPubMed 36597993
- Cells 12:2022-Inositol Supplementation Alleviates Cisplatin-Induced Acute Kidney Injury via Inhibition of Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyue Qi,Fei Deng,Yinghuai Wang,Hao Zhang,Yashpal S Kanwar,Yingbo DaiPubMed 36611810
- Nature communications 13:78152022Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhikai Wang,Yang Sun,Fangzhou Lou,Jing Bai,Hong Zhou,Xiaojie Cai,Libo Sun,Qianqian Yin,Sibei Tang,Yue Wu,Li Fan,Zhenyao Xu,Hong Wang,Xiaoyu Hu,Honglin WangPubMed 36535970
- Journal of neuroinflammation 19:2432022Treadmill exercise promotes E3 ubiquitin ligase to remove amyloid β and P-tau and improve cognitive ability in APP/PS1 transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLongfei Xu,Mingzhe Li,Aili Wei,Miaomiao Yang,Chao Li,Ran Liu,Yuejun Zheng,Yuxin Chen,Zixi Wang,Kun Wang,Tianhui WangPubMed 36195875
- iScience 25:1047732022Chemoproteomics reveals berberine directly binds to PKM2 to inhibit the progression of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Hai Yan,Li-Mu Hu,Xue-Hui Hao,Jiang Liu,Xi-Ying Tan,Zhi-Rong Geng,Jing Ma,Zhi-Lin WangPubMed 35992091
- PLoS pathogens 18:e10102042022Bay41-4109-induced aberrant polymers of hepatitis b capsid proteins are removed via STUB1-promoted p62-mediated macroautophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiacheng Lin,Limin Yin,Xia-Zhen Xu,He-Chen Sun,Zhi-Hua Huang,Xue-Yun Ni,Yan Chen,Xu LinPubMed 35030230
- Aging 13:25564-255772021CHIP ameliorates cerebral ischemia-reperfusion injury by attenuating necroptosis and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDabao Yao,Shuo Zhang,Zhengwei Hu,Haiyang Luo,Chengyuan Mao,Yu Fan,Mibo Tang,Fen Liu,Si Shen,Liyuan Fan,Mengjie Li,Jingjing Shi,Jiadi Li,Dongrui Ma,Yuming Xu,Changhe ShiPubMed 34905731
- Scientific reports 11:147262021Investigation of energy metabolic dynamism in hyperthermia-resistant ovarian and uterine cancer cells under heat stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaisei Kanamori,Natumi Miyazaki,Shigeki Aoki,Kousei Ito,Akihiro Hisaka,Hiroto HatakeyamaPubMed 34282188
- Journal of cellular and molecular medicine 25:5976-59872021NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Hu,Shijie Zhou,Xuefeng Hu,Hua Zhang,Xiaorong Lan,Mei Li,Yunbing Wang,Qinsheng HuPubMed 33993634
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/120 | Notes For unpurified, use 1/10 - 1/100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation (PubMed:10330192, PubMed:11146632, PubMed:11557750, PubMed:23990462, PubMed:26265139). Plays a role in the maintenance of mitochondrial morphology and promotes mitophagic removal of dysfunctional mitochondria; thereby acts as a protector against apoptosis in response to cellular stress (By similarity). Negatively regulates vascular smooth muscle contraction, via degradation of the transcriptional activator MYOCD and subsequent loss of transcription of genes involved in vascular smooth muscle contraction (By similarity). Promotes survival and proliferation of cardiac smooth muscle cells via ubiquitination and degradation of FOXO1, resulting in subsequent repression of FOXO1-mediated transcription of pro-apoptotic genes (PubMed:19483080). Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes (PubMed:20724525). Inhibits lipopolysaccharide-induced apoptosis and hypertrophy in cardiomyocytes, via ubiquitination and subsequent proteasomal degradation of NFATC3 (PubMed:30980393). Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (PubMed:10330192, PubMed:11146632, PubMed:11557750, PubMed:23990462). Ubiquitinates NOS1 in concert with Hsp70 and Hsp40 (PubMed:15466472). Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90 (PubMed:10330192, PubMed:11146632, PubMed:15466472). Ubiquitinates CHRNA3 targeting it for endoplasmic reticulum-associated degradation in cortical neurons, as part of the STUB1-VCP-UBXN2A complex (PubMed:26265139). Ubiquitinates and promotes ESR1 proteasomal degradation in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury (By similarity). Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation (PubMed:11557750, PubMed:23990462). Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome (PubMed:19713937). Mediates polyubiquitination of CYP3A4 (PubMed:19103148). Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation (PubMed:19567782). Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation (PubMed:27708256). Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner (PubMed:23973223). Catalyzes monoubiquitination of SIRT6, preventing its degradation by the proteasome (PubMed:24043303). Likely mediates polyubiquitination and down-regulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity (PubMed:28813410). Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation (PubMed:24613385). Plays a role in the degradation of TP53 (PubMed:26634371). Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation (PubMed:29883609). May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820). Ubiquitinates PPARG in macrophages playing a role in M2 macrophages polarization and angiogenesis (By similarity).
Alternative names
CHIP, PP1131, STUB1, E3 ubiquitin-protein ligase CHIP, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, RING-type E3 ubiquitin transferase CHIP, STIP1 homology and U box-containing protein 1
Recommended products
Rabbit Recombinant Monoclonal STUB1/CHIP antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 20 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4447
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STUB1 also known as CHIP (C-Terminal of Hsc70 Interacting Protein) is a protein with a molecular mass of approximately 35 kDa. It has ubiquitin ligase activity and functions as a co-chaperone. STUB1 interacts with chaperone proteins such as Hsp70 and Hsp90 modulating their activity. You can observe STUB1 expression in various tissues with higher levels found in the brain heart and skeletal muscle.
- Biological function summary
STUB1 plays an important role in protein quality control by targeting misfolded or damaged proteins for degradation. It forms a complex with chaperone proteins Hsp70 and Hsp90 facilitating the ubiquitination process. This helps maintain cellular proteostasis. STUB1 has a critical role in preventing protein aggregation which is important in stress responses and normal cellular function.
- Pathways
STUB1 has significant involvement in the protein degradation pathway and the heat shock response pathway. It works closely with proteasome-associated proteins to ensure proteostasis. In these pathways STUB1 interacts with other proteins such as Hsc70 and Hsp70 which assist in protein folding and stability under stress conditions.
- Associated diseases and disorders
STUB1 has links to neurodegenerative diseases and cancer. A dysfunction in STUB1 can lead to protein aggregation a hallmark of neurodegenerative conditions like Alzheimer's disease. In certain cancers altered STUB1 expression might affect cell survival pathways with related proteins including Hsp70 and Hsp90. Therapeutic strategies that modulate STUB1 and its interactions may provide avenues for disease intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
![Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunoprecipitation-img29401.jpg "Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
ab134064 (purified) at 1/120 immunoprecipitating STUB1/CHIP in 10 μg HeLa (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Predicted band size: 35 kDa
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img17351.jpg "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/20000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg
Lane 3: HEK293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunocytochemistry-immunofluorescence-img129457.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Immunofluorescence staining of SH-SY5Y cells with purified ab134064 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab134064 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling STUB1/CHIP with unpurified ab134064 at 1/100 dilution.
![Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--flow-cytometry-intracellular-img80372.jpg "Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Intracellular Flow Cytometry analysis of SH-SY5Y (Human epithelial cell line from embryonic kidney) cells labeling STUB1/CHIP with purified ab134064 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img142527.jpg "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/10000 dilution
All lanes: Mouse brain at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img2198.jpg "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
All lanes: Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/1000 dilution
Lane 1: Wild type primary Mouse neurons whole cell lysate at 20 µg
Lane 2: STUB1 knockout primary Mouse neurons whole cell lysate at 20 µg
Lane 3: Wild type MEF (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 4: STUB1 knockout MEF (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Exposure time: 25s
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img65575.jpg "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/50000 dilution
All lanes: Rat brain at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img125427.jpg "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
ECV-304 (derived from T24, discontinued by ATCC)
All lanes: Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/10000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2: MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: ECV-304 (Human urinary bladder cancer cell line) cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img142527.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img2198.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img65575.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
![Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064), expandable thumbnail](https://content.abcam.com/products/images/stub1-chip-antibody-epr4447-ab134064--western-blot-img125427.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)")
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