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AB229646

Anti-STX17 antibody

5

(1 Review)

|

(16 Publications)

Rabbit Polyclonal STX17 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STX17.

View Alternative Names

Syntaxin-17, STX17

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (AB229646)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (AB229646)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling STX17 protein using ab229646 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).
Scale bar = 10 µM

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STX17 antibody (AB229646)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STX17 antibody (AB229646)

Paraffin-embedded mouse prostate tissue staining STX17 using ab229646 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (AB229646)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (AB229646)

Immunofluorescence analysis of 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons labeling STX17 (green) with ab229646 at 1/500 dilution. Red : beta Tubulin 3, using beta Tubulin 3 antibody at 1/500 dilution. Blue : DAPI.

Western blot - Anti-STX17 antibody (AB229646)
  • WB

Supplier Data

Western blot - Anti-STX17 antibody (AB229646)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-STX17 antibody (ab229646) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 33 kDa

false

Western blot - Anti-STX17 antibody (AB229646)
  • WB

Supplier Data

Western blot - Anti-STX17 antibody (AB229646)

12% SDS-PAGE

All lanes:

Western blot - Anti-STX17 antibody (ab229646) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human STX17. The exact immunogen used to generate this antibody is proprietary information.

P56962

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STX17 also known as Syntaxin 17 is a SNARE protein involved in membrane fusion processes. Its molecular weight is approximately 34 kDa. STX17 is predominantly expressed in the endoplasmic reticulum and associated membranes. The protein plays a mechanical role in mediating the fusion of vesicles with target membranes facilitating the transport of molecules within cells. STX17 localizes mostly in cell types associated with high metabolic activity where it supports cellular trafficking demands.
Biological function summary

Syntaxin 17 contributes to the formation of a multiprotein complex involved in the fusion of autophagosomes with lysosomes. This process is important for macroautophagy where damaged organelles and proteins are degraded and recycled. The protein forms a functional unit with other SNARE proteins including VAMP8 and SNAP29 to carry out its role. It ensures cellular homeostasis by facilitating the clearance of unnecessary or damaged cellular components under stress conditions.

Pathways

Syntaxin 17 is critical in the autophagy and endocytosis pathways. These pathways are involved in the degradation and recycling of cellular components maintaining cellular integrity and energy balance. In the autophagy pathway STX17 functions alongside proteins such as LC3 and Beclin-1 coordinating the recognition and processing of autophagosomes. In the endocytosis pathway its interactions help balance the intake and processing of extracellular materials.

STX17 shows significant connections to neurodegenerative disorders and cancer. Its dysfunction in the autophagy process may lead to accumulation of toxic protein aggregates contributing to disorders like Alzheimer's disease. Defects or mutations in the functioning of STX17 have been linked to impaired cellular waste disposal in this context. In cancer alterations in STX17 expression or function can disrupt autophagic processes and affect cancer cell survival. Understanding its link with neurodegenerative proteins such as tau could offer insights for therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502). STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502, PubMed : 28504273). May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed : 21545355).
See full target information STX17
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:975-988 PubMed40191726

2025

COPB2 promotes hepatocellular carcinoma progression through regulation of YAP1 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Wu,Xianlin Guo,Zhishi Wu,Liang Chen,Suqing Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2414960 PubMed40135829

2025

Syntaxin 17 Translocation Mediated Mitophagy Switching Drives Hyperglycemia-Induced Vascular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Luo,Rui Wang,Jingwen Gong,Shuting Wang,Chuan Yun,Zongcun Chen,Yanan Jiang,Xiaoquan Liu,Haofu Dai,Haochen Liu,Yunsi Zheng

Nature communications 15:8519 PubMed39353976

2024

CHIP ameliorates nonalcoholic fatty liver disease via promoting K63- and K27-linked STX17 ubiquitination to facilitate autophagosome-lysosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjin Rho,Seungyeon Kim,Seung Up Kim,Jeong Won Kim,Sang Hoon Lee,Sang Hoon Park,Freddy E Escorcia,Joon-Yong Chung,Jaewhan Song

Acta neuropathologica communications 12:82 PubMed38812004

2024

Axonal autophagic vesicle transport in the rat optic nerve in vivo under normal conditions and during acute axonal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyue Luo,Jiong Zhang,Johan Tolö,Sebastian Kügler,Uwe Michel,Mathias Bähr,Jan Christoph Koch

International journal of molecular sciences 25: PubMed38396708

2024

Exploring the Importance of Differential Expression of Autophagy Markers in Term Placentas from Late-Onset Preeclamptic Pregnancies.

Applications

Unspecified application

Species

Unspecified reactive species

Luis M Garcia-Puente,Cielo García-Montero,Oscar Fraile-Martinez,Julia Bujan,Juan A De León-Luis,Coral Bravo,Patrocinio Rodríguez-Benitez,Laura López-González,Raul Díaz-Pedrero,Melchor Álvarez-Mon,Natalio García-Honduvilla,Miguel A Saez,Miguel A Ortega

Molecular medicine (Cambridge, Mass.) 30:4 PubMed38172666

2024

Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Miaoyang Hu,Jingrong Chen,Xinyu Lou,Hongchao Zhang,Muhan Li,Jie Cheng,Tengfei Ma,Jianping Xiong,Rong Gao,Xufeng Chen,Jun Wang

Theranostics 14:75-95 PubMed38164137

2024

MK8722 initiates early-stage autophagy while inhibiting late-stage autophagy via FASN-dependent reprogramming of lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Luhui Wang,Haiyan Zhu,Zhehao Shi,Bo Chen,Huirong Huang,Ganglian Lin,Jiacheng Li,Haitao Yu,Shihao Xu,Gang Chen,Rongying Ou,Chunxiu Dai

Redox biology 69:102972 PubMed38056310

2023

ALS-linked SOD1 mutations impair mitochondrial-derived vesicle formation and accelerate aging.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Guo,Teng Guan,Qiang Yu,Nitesh Sanghai,Kashfia Shafiq,Meiyu Li,Xin Jiao,Donghui Na,Guohui Zhang,Jiming Kong

Materials today. Bio 21:100700 PubMed37455821

2023

Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of to alleviate tissue infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Peilin,Peng Ying,Wang Renyuan,Li Zhuoxuan,Yang Zhenwu,Zhao Mai,Song Jianguo,Zhang Hao,Yin Gang,Lin Lin,Lin Haodong

Proceedings of the National Academy of Sciences of the United States of America 119:e2202730119 PubMed36044553

2022

Extended-synaptotagmin 1 engages in unconventional protein secretion mediated via SEC22B vesicle pathway in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kohji Yamada,Saya Motohashi,Tsunekazu Oikawa,Naoko Tago,Rei Koizumi,Masaya Ono,Toshiaki Tachibana,Ayano Yoshida,Saishu Yoshida,Masayuki Shimoda,Masahiro Oka,Yoshihiro Yoneda,Kiyotsugu Yoshida
View all publications
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