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AB245637

Anti-STX17 antibody

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(1 Publication)

Rabbit Polyclonal STX17 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STX17 aa 100-150.

View Alternative Names

Syntaxin-17, STX17

1 Images
Immunoprecipitation - Anti-STX17 antibody (AB245637)
  • IP

Supplier Data

Immunoprecipitation - Anti-STX17 antibody (AB245637)

STX17 was immunoprecipitated from HEK-293T whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab245637. Western blot was performed from the immunoprecipitate using ab245637 at 1/1000 dilution.

Lane 1 : HEK-293T lysate (Input).
Lane 2 : ab245638 IP in HEK-293T lysate.
Lane 3 : Control IgG in HEK-293T whole cell lysate

Chemiluminescence with an exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-STX17 antibody (ab245637)

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human STX17 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P56962

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "8-15 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }
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Product details

ab245637 has not performed satisfactorily when used for WB of STX17 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of STX17.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245637 was affinity purified using an epitope specific to STX17 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STX17 also known as Syntaxin 17 is a SNARE protein involved in membrane fusion processes. Its molecular weight is approximately 34 kDa. STX17 is predominantly expressed in the endoplasmic reticulum and associated membranes. The protein plays a mechanical role in mediating the fusion of vesicles with target membranes facilitating the transport of molecules within cells. STX17 localizes mostly in cell types associated with high metabolic activity where it supports cellular trafficking demands.
Biological function summary

Syntaxin 17 contributes to the formation of a multiprotein complex involved in the fusion of autophagosomes with lysosomes. This process is important for macroautophagy where damaged organelles and proteins are degraded and recycled. The protein forms a functional unit with other SNARE proteins including VAMP8 and SNAP29 to carry out its role. It ensures cellular homeostasis by facilitating the clearance of unnecessary or damaged cellular components under stress conditions.

Pathways

Syntaxin 17 is critical in the autophagy and endocytosis pathways. These pathways are involved in the degradation and recycling of cellular components maintaining cellular integrity and energy balance. In the autophagy pathway STX17 functions alongside proteins such as LC3 and Beclin-1 coordinating the recognition and processing of autophagosomes. In the endocytosis pathway its interactions help balance the intake and processing of extracellular materials.

STX17 shows significant connections to neurodegenerative disorders and cancer. Its dysfunction in the autophagy process may lead to accumulation of toxic protein aggregates contributing to disorders like Alzheimer's disease. Defects or mutations in the functioning of STX17 have been linked to impaired cellular waste disposal in this context. In cancer alterations in STX17 expression or function can disrupt autophagic processes and affect cancer cell survival. Understanding its link with neurodegenerative proteins such as tau could offer insights for therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502). STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane (PubMed : 23217709, PubMed : 25686604, PubMed : 28306502, PubMed : 28504273). May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed : 21545355).
See full target information STX17
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:435 PubMed36316321

2022

CUR5g, a novel autophagy inhibitor, exhibits potent synergistic anticancer effects with cisplatin against non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxuan Chen,Yunpeng Shen,Bowen Wu,Peichang Yang,Gangchun Sun,Xiaoting Liu,Pengfei Qiang,Yamei Gao,Fangfang Sha,Zirui Li,Lu Zhang
View all publications
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