Anti-STX17 antibody [HL3534] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STX17 antibody [HL3534] - BSA and Azide free (AB327518)

ab327518 detects STX17 protein by immunohistochemical analysis.
Sample : Paraffin-embedded mouse testis.
STX17 stained by ab327518 diluted at 1 : 200.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (AB327518)

Wild-type (WT) and STX17 knockout (KO) HeLa cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ab327518 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (ab327518) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 5 µg

Lane 2:

STX17 knockout (KO) HeLa cell extracts at 5 µg

true

• WB

Supplier Data

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (AB327518)

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ab327518 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (ab327518) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

Hela whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

false

• WB

Supplier Data

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (AB327518)

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ab327518 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-STX17 antibody [HL3534] - BSA and Azide free (ab327518) at 1/1000 dilution

Lane 2:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

false