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Rabbit Recombinant Monoclonal STYX antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-STYX antibody [EPR16314] (AB205200), expandable thumbnail
  • Western blot - Anti-STYX antibody [EPR16314] (AB205200), expandable thumbnail
  • Western blot - Anti-STYX antibody [EPR16314] (AB205200), expandable thumbnail
  • Western blot - Anti-STYX antibody [EPR16314] (AB205200), expandable thumbnail
  • Western blot - Anti-STYX antibody [EPR16314] (AB205200), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Expected
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Catalytically inactive phosphatase (PubMed:23847209). Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2) (PubMed:23847209). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (PubMed:23847209). By binding to the F-box of FBXW7, prevents the assembly of FBXW7 into the SCF E3 ubiquitin-protein ligase complex, and thereby inhibits degradation of its substrates (PubMed:28007894). Plays a role in spermatogenesis (By similarity).

Alternative names

Serine/threonine/tyrosine-interacting protein, Inactive tyrosine-protein phosphatase STYX, Phosphoserine/threonine/tyrosine interaction protein, STYX

Recommended products

Rabbit Recombinant Monoclonal STYX antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16314
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein STYX (Serine/threonine/tyrosine-interacting protein) also known as PTPN23 functions as a pseudo-phosphatase meaning it has structural resemblance to phosphatases but lacks catalytic activity. STYX acts by binding to substrates and competing with active phosphatases. The molecular mass of STYX is approximately 55 kDa. Expression of STYX occurs widely found in tissues such as brain heart and testis as well as in various transformed cell lines.

Biological function summary

STYX contributes to cellular processes like signal transduction and development by modulating signaling pathways through its interaction with phosphorylated tyrosines. Although not part of a functional enzyme complex due to its pseudo-activity STYX interacts with components involved in kinase signaling. These interactions can act as a regulatory mechanism impacting various cellular functions.

Pathways

STYX influences several signaling cascades particularly the MAPK and Wnt pathways. It exerts its effects by interacting with proteins such as MAP kinase kinase (MEK) and β-catenin integral components of these pathways. Through these interactions STYX affects cell proliferation differentiation and apoptosis indicating its important role in cellular communication and response mechanisms.

Associated diseases and disorders

Aberrant regulation of STYX associates with cancer and neurodegenerative diseases. In cancer overexpression or mutation of STYX can impact the MAPK pathway leading to dysregulation of cell division and growth. Additionally in neurodegenerative disorders like Alzheimer's disease altered STYX function relates to abnormal phosphorylation states and interactions with proteins such as Tau contributing to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Western blot - Anti-STYX antibody [EPR16314] (ab205200)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/5000 dilution

    All lanes: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 1min

  • Western blot - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Western blot - Anti-STYX antibody [EPR16314] (ab205200)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Lane 2: Human fetal liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 1min

  • Western blot - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Western blot - Anti-STYX antibody [EPR16314] (ab205200)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    All lanes: Human lymph node lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 3min

  • Western blot - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Western blot - Anti-STYX antibody [EPR16314] (ab205200)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1: Mouse spleen lysate at 10 µg

    Lane 2: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 1min

  • Western blot - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Western blot - Anti-STYX antibody [EPR16314] (ab205200)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (ab205200)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling STYX with ab205200 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab205200 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunoprecipitation - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Immunoprecipitation - Anti-STYX antibody [EPR16314] (ab205200)

    STYX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab205200 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205200 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10ug (Input). Lane 2: ab205200 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab205200 in NIH/3T3 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-STYX antibody [EPR16314] (ab205200)

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (ab205200)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab205200 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-STYX antibody [EPR16314] (ab205200), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STYX antibody [EPR16314] (ab205200)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

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Product protocols

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