AB205200

Anti-STYX antibody [EPR16314]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal STYX antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

Serine/threonine/tyrosine-interacting protein, Inactive tyrosine-protein phosphatase STYX, Phosphoserine/threonine/tyrosine interaction protein, STYX

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (AB205200)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (AB205200)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling STYX with ab205200 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab205200 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (AB205200)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab205200 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-STYX antibody [EPR16314] (AB205200)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STYX antibody [EPR16314] (AB205200)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-STYX antibody [EPR16314] (AB205200)

STYX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab205200 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205200 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10ug (Input). Lane 2 : ab205200 IP in NIH/3T3 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205200 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-STYX antibody [EPR16314] (ab205200)

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/5000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

All lanes:

Human lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16314

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein STYX (Serine/threonine/tyrosine-interacting protein) also known as PTPN23 functions as a pseudo-phosphatase meaning it has structural resemblance to phosphatases but lacks catalytic activity. STYX acts by binding to substrates and competing with active phosphatases. The molecular mass of STYX is approximately 55 kDa. Expression of STYX occurs widely found in tissues such as brain heart and testis as well as in various transformed cell lines.

Biological function summary

STYX contributes to cellular processes like signal transduction and development by modulating signaling pathways through its interaction with phosphorylated tyrosines. Although not part of a functional enzyme complex due to its pseudo-activity STYX interacts with components involved in kinase signaling. These interactions can act as a regulatory mechanism impacting various cellular functions.

Pathways

STYX influences several signaling cascades particularly the MAPK and Wnt pathways. It exerts its effects by interacting with proteins such as MAP kinase kinase (MEK) and β-catenin integral components of these pathways. Through these interactions STYX affects cell proliferation differentiation and apoptosis indicating its important role in cellular communication and response mechanisms.

Aberrant regulation of STYX associates with cancer and neurodegenerative diseases. In cancer overexpression or mutation of STYX can impact the MAPK pathway leading to dysregulation of cell division and growth. Additionally in neurodegenerative disorders like Alzheimer's disease altered STYX function relates to abnormal phosphorylation states and interactions with proteins such as Tau contributing to disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalytically inactive phosphatase (PubMed : 23847209). Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2) (PubMed : 23847209). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (PubMed : 23847209). By binding to the F-box of FBXW7, prevents the assembly of FBXW7 into the SCF E3 ubiquitin-protein ligase complex, and thereby inhibits degradation of its substrates (PubMed : 28007894). Plays a role in spermatogenesis (By similarity).

See full target information Serine/threonine/tyrosine-interacting protein

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Dose-response : a publication of International Hormesis Society 23:15593258251335814 PubMed40416644

2025

Apigenin Inhibits Cell Ferroptosis by Activating the PI3K/Akt Pathway and Alleviates Renal Injury Caused by Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Haina Zhang,Yanhua Cao,Liting Jiao,Jianwei Wan

Cell death & disease 13:268 PubMed35338113

2022

Pseudophosphatase STYX is induced by Helicobacter pylori and promotes gastric cancer progression by inhibiting FBXO31 function.

Applications

Unspecified application

Species

Unspecified reactive species

Jiansong Liu,Yichen Zang,Cunying Ma,Dandan Wang,Zhuangfei Tian,Xia Xu,Wenjuan Li,Jihui Jia,Zhifang Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STYX antibody [EPR16314]

Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (AB205200)

Immunocytochemistry/ Immunofluorescence - Anti-STYX antibody [EPR16314] (AB205200)

Flow Cytometry (Intracellular) - Anti-STYX antibody [EPR16314] (AB205200)

Immunoprecipitation - Anti-STYX antibody [EPR16314] (AB205200)

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Western blot - Anti-STYX antibody [EPR16314] (AB205200)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise