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Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human TAC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20366

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAIHC-PICC/IF
Human
Expected
Predicted
Predicted
Mouse
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Chicken
Predicted
Predicted
Predicted
Common marmoset
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Guinea pig
Expected
Predicted
Predicted
Hamster
Predicted
Predicted
Predicted
Horse
Predicted
Predicted
Predicted
Shark
Predicted
Predicted
Predicted

Expected
Expected

Species
Mouse, Rat, Guinea pig, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Horse, Chicken, Hamster, Cow, Shark, Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Alternative names

Recommended products

Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human TAC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20366
Clone number
SP-DE4-21
Purification technique
Affinity purification Protein G
Specificity

The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.

Biological function summary

This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.

Pathways

Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.

Associated diseases and disorders

Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)

    ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)

    ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail
    Image courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)

    ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)

    Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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