Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43466.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)")
![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunocytochemistry-immunofluorescence-img17867.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (AB14184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133251.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46900.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)")
Publications
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- Frontiers in physiology 14:11980662023Adventitial macrophage accumulation impairs perivascular nerve function in mesenteric arteries with inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Grunz,Benjamin W Jones,Olubodun Michael Lateef,Sidharth Sen,Katie Wilkinson,Trupti Joshi,Erika M BoermanPubMed 37342800
- Journal of veterinary science 24:e262023Inhibition of angiotensin converting enzyme increases PKCβI isoform expression via activation of substance P and bradykinin receptors in cultured astrocytes of mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae-Gyun Choi,Sheu-Ran Choi,Dong-Wook Kang,Hyun Jin Shin,Miae Lee,Jungmo Hwang,Hyun-Woo KimPubMed 37012034
- International journal of molecular sciences 24:2023Spinal Cord Sensitization and Spinal Inflammation from an In Vivo Rat Endplate Injury Associated with Painful Intervertebral Disc Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlon Lai,Denise Iliff,Kashaf Zaheer,Dalin Wang,Jennifer Gansau,Damien M Laudier,Venetia Zachariou,James C IatridisPubMed 36834838
- Nature communications 13:79132022Parabrachial-to-parasubthalamic nucleus pathway mediates fear-induced suppression of feeding in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Nagashima,Suguru Tohyama,Kaori Mikami,Masashi Nagase,Mieko Morishima,Atsushi Kasai,Hitoshi Hashimoto,Ayako M WatabePubMed 36585411
- The Journal of veterinary medical science 85:123-1342022Histological study on regional specificity of the mucosal nerve network in the rat large intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoki Nakanishi,Youhei Mantani,Nobuhiko Ohno,Rinako Morishita,Toshifumi Yokoyama,Nobuhiko HoshiPubMed 36517005
- Frontiers in bioengineering and biotechnology 10:9249182022Raloxifene Stimulates Estrogen Signaling to Protect Against Age- and Sex-Related Intervertebral Disc Degeneration in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeharika Bhadouria,Alycia G Berman,Joseph M Wallace,Nilsson HolguinPubMed 36032728
- Drug design, development and therapy 16:1349-13632022Network Pharmacology Analysis of Hewei Jiangni Granule for Gastroesophageal Reflux Disease and Experimental Verification of Its Anti-Neurogenic Inflammation Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Cheng,Fushun Kou,Xiaosi Zhang,Yi Dai,Lei Shi,Chune Xie,Xiaohong Li,Junxiang LiPubMed 35547866
- International journal of molecular sciences 23:2022The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Ching Lu,Ying-Yi Lu,Hung-Pei Tsai,Chieh-Hsin WuPubMed 35216130
- Iranian journal of basic medical sciences 24:657-6652021Effects of the diphenylheptane extract of rhizomes on ethanol-induced gastric ulcers in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiwen Lin,Huijuan Qu,Yinfeng Tan,Tang Deng,Bingmiao Gao,Na WeiPubMed 34249268
- American journal of physiology. Heart and circulatory physiology 320:H1887-H19022021Role of perivascular nerve and sensory neurotransmitter dysfunction in inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles E Norton,Elizabeth A Grunz-Borgmann,Marcia L Hart,Benjamin W Jones,Craig L Franklin,Erika M BoermanPubMed 33710922
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human TAC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20366
Reactivity data
Select an application| ELISA | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Predicted | Predicted |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted |
| Common marmoset | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Guinea pig | Expected | Predicted | Predicted |
| Hamster | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Shark | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Chicken, Hamster, Cow, Shark, Common marmoset | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Alternative names
NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT
Recommended products
Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human TAC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20366
- Clone number
- SP-DE4-21
- Purification technique
- Affinity purification Protein G
- Specificity
The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.
- Biological function summary
This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.
- Pathways
Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.
- Associated diseases and disorders
Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43466.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
This image is courtesy of a customer review submitted by Carl HobbsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunocytochemistry-immunofluorescence-img17867.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)
ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133251.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Image courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184), expandable thumbnail](https://content.abcam.com/products/images/substance-p-antibody-sp-de4-21-ab14184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46900.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
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