Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004
pH: 7.4
Constituents: 100% PBS
ELISA | IHC-P | ICC/IF | |
---|---|---|---|
Human | Expected | Predicted | Predicted |
Mouse | Expected | Tested | Expected |
Rat | Expected | Expected | Tested |
Chicken | Predicted | Predicted | Predicted |
Common marmoset | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Guinea pig | Expected | Predicted | Predicted |
Hamster | Predicted | Predicted | Predicted |
Horse | Predicted | Predicted | Predicted |
Shark | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Guinea pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Horse, Chicken, Hamster, Cow, Shark, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Horse, Chicken, Guinea pig, Hamster, Cow, Shark, Common marmoset, Human | Dilution info - | Notes - |
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Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT
Anti-Substance P antibody [SP-DE4-21] ab14184 is a mouse monoclonal antibody that is used in Substance P IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Antibody clone SP-DE4-21 has been tried and trusted by researchers since 2004
pH: 7.4
Constituents: 100% PBS
The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.
This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.
Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.
Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.
Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
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