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AB250666

Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SUCLA2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ATP-specific succinyl-CoA synthetase subunit beta, Itaconyl--CoA ligase [ADP-forming] subunit beta, Malyl--CoA ligase [ADP-forming] subunit beta, Succinyl-CoA synthetase beta-A chain, A-SCS, SCS-betaA, SUCLA2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)

This data was developed using ab183513, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)

This data was developed using ab183513, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)

This data was developed using ab183513, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)
  • IP

Supplier Data

Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)

This data was developed using ab183513, the same antibody clone in a different buffer formulation.

Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (<a href='/en-us/products/primary-antibodies/sucla2-antibody-epr14925-ab183513'>ab183513</a>)

Predicted band size: 50 kDa

false

Western blot - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)
  • WB

Supplier Data

Western blot - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (AB250666)

This data was developed using ab183513, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SUCLA2 antibody [EPR14925] (<a href='/en-us/products/primary-antibodies/sucla2-antibody-epr14925-ab183513'>ab183513</a>) at 1/50000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Molt-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14925

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 0
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Primary Antibodies

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Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250666 is the carrier-free version of ab183513.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUCLA2 also known as succinate-CoA ligase (ADP-forming) subunit beta is an enzyme with a molecular mass of approximately 48 kDa. It plays an important role in the mitochondrial matrix where it participates in the citric acid cycle. The enzyme is found in various tissues with higher expression in muscle and brain tissues. SUCLA2 functions as part of the succinyl-CoA synthetase complex which catalyzes the reversible conversion of succinyl-CoA to succinate with the concurrent substrate-level phosphorylation of GDP to GTP or ADP to ATP depending on tissue type.
Biological function summary

The SUCLA2 subunit is essential for energy metabolism. It forms a component of the succinyl-CoA synthetase complex working with the alpha subunit SUCLG1. This complex supports the provision of energy by converting GDP to GTP in processes needing high energy levels critical for maintaining cellular homeostasis. Therefore it especially supports tissues with high energy demands. The action of SUCLA2 links energy production with the metabolic pathways involved in cellular respiration.

Pathways

SUCLA2 plays a critical role in the citric acid cycle also known as the Krebs cycle. Part of this cycle SUCLA2 contributes to cellular respiration and energy production. The SUCLA2 protein closely interacts with SUCLG1 to form the functional succinyl-CoA synthetase enzyme. Additionally it shows connections to the electron transport chain where the products of its catalysis are used in ATP generation. Defects in these pathways can have widespread effects on cellular energy states and mitochondrial function.

SUCLA2 mutations are linked to mitochondrial DNA depletion syndromes particularly myopathy and encephalopathy. These conditions lead to severe energy metabolism impairments due to faulty mitochondrial function. SUCLA2 has also been associated with Leigh syndrome a neurodegenerative disorder marked by compromised cellular respiration. Such disorders point towards deficient ATP synthesis highlighting the interconnected role of SUCLA2 with other mitochondrial proteins involved in cellular energy metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed : 15877282, PubMed : 34492704, PubMed : 40108300). The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit (By similarity). Also able to act as an ATP-specific itaconyl- and malyl-CoA synthetase (PubMed : 40108300).
See full target information Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230639 PubMed36820063

2023

Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Gang Jin,Shihong Chen,Jing Luo,Wei Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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