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AB204432

Anti-SUCLG1 antibody

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(2 Publications)

Rabbit Polyclonal SUCLG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUCLG1 aa 100-200.

View Alternative Names

Itaconyl--CoA ligase [ADP/GDP-forming] subunit alpha, Malyl--CoA ligase [ADP/GDP-forming] subunit alpha, Succinyl-CoA synthetase subunit alpha, SCS-alpha, SUCLG1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)

Paraffin embedded human kidney tissue stained for SUCLG1 using ab204432 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)

Paraffin embedded human Fallopian tube tissue stained for SUCLG1 using ab204432 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)

Paraffin embedded human cerebellum tissue stained for SUCLG1 using ab204432 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Western blot - Anti-SUCLG1 antibody (AB204432)
  • WB

Supplier Data

Western blot - Anti-SUCLG1 antibody (AB204432)

All lanes:

Western blot - Anti-SUCLG1 antibody (ab204432) at 1/100 dilution

Lane 1:

RT4 lysate

Lane 2:

U-251 MG lysate

Predicted band size: 36 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody (AB204432)

Paraffin embedded human stomach tissue stained for SUCLG1 using ab204432 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SUCLG1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P53597

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUCLG1 also known as succinate-CoA ligase (GDP-forming) subunit alpha participates in cellular energy metabolism. It catalyzes the conversion of succinyl-CoA to succinate in the mitochondria linking the citric acid cycle and oxidative phosphorylation. SUCLG1 has a molecular mass of approximately 32 kDa. This protein expresses highly in metabolically active tissues such as the liver kidney and brain where energy demand is significant.
Biological function summary

Succinate-CoA ligase facilitates the synthesis of nucleoside triphosphates within the mitochondria by participating in the GDP-specific form of the enzyme complex. SUCLG1 forms a heterodimer with SUCLG2 (the beta subunit) creating succinate-CoA ligase's functional enzyme complex. Through its action SUCLG1 directly impacts the generation of GTP which plays a critical role in modulating metabolic reactions within cells.

Pathways

Succinate-CoA ligase integrates into the citric acid cycle and plays a significant role in cellular respiration. By converting succinyl-CoA to succinate it helps sustain the cycle's continued operation. The protein also links with nucleoside diphosphate kinase which governs the maintenance of cellular nucleotide balance. These interactions highlight the essential nature of SUCLG1 in maintaining energy flow within cells.

Mutations in SUCLG1 associate with mitochondrial DNA depletion syndromes which severely affect muscle and brain tissue. Dysfunction within succinate-CoA ligase disrupts the energy production process leading to cellular energy deficits. Additionally this protein’s interaction with SUCLG2 implies potential implications in similar disorders related to mitochondrial function showing the importance of their combined activity in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed : 34492704, PubMed : 40108300). The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits (By similarity). Also able to act as an itaconyl- and malyl-CoA synthetase (PubMed : 40108300).
See full target information SUCLG1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe

JCI insight 5: PubMed32271165

2020

IMPDH inhibitors for antitumor therapy in tuberous sclerosis complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Valvezan,Molly C McNamara,Spencer K Miller,Margaret E Torrence,John M Asara,Elizabeth P Henske,Brendan D Manning
View all publications
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Product promise

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