AB97867

Anti-SUCLG1 antibody

Name: Anti-SUCLG1 antibody (ab97867) | Abcam
Brand: Abcam Limited
SKU: AB97867

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(6 Publications)

Rabbit Polyclonal SUCLG1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUCLG1 aa 1 to C-terminus.

View Alternative Names

Succinyl-CoA synthetase subunit alpha, SCS-alpha, SUCLG1

3 Images

Supplier Data

Western blot - Anti-SUCLG1 antibody (AB97867)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SUCLG1 antibody (ab97867) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-SUCLG1 antibody (AB97867)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SUCLG1 antibody (ab97867) at 1/10000 dilution

All lanes:

Mouse brain tissue lysates at 20 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-SUCLG1 antibody (AB97867)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SUCLG1 antibody (ab97867) at 1/3000 dilution

All lanes:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SUCLG1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P53597

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUCLG1 also known as succinate-CoA ligase (GDP-forming) subunit alpha participates in cellular energy metabolism. It catalyzes the conversion of succinyl-CoA to succinate in the mitochondria linking the citric acid cycle and oxidative phosphorylation. SUCLG1 has a molecular mass of approximately 32 kDa. This protein expresses highly in metabolically active tissues such as the liver kidney and brain where energy demand is significant.

Biological function summary

Succinate-CoA ligase facilitates the synthesis of nucleoside triphosphates within the mitochondria by participating in the GDP-specific form of the enzyme complex. SUCLG1 forms a heterodimer with SUCLG2 (the beta subunit) creating succinate-CoA ligase's functional enzyme complex. Through its action SUCLG1 directly impacts the generation of GTP which plays a critical role in modulating metabolic reactions within cells.

Pathways

Succinate-CoA ligase integrates into the citric acid cycle and plays a significant role in cellular respiration. By converting succinyl-CoA to succinate it helps sustain the cycle's continued operation. The protein also links with nucleoside diphosphate kinase which governs the maintenance of cellular nucleotide balance. These interactions highlight the essential nature of SUCLG1 in maintaining energy flow within cells.

Mutations in SUCLG1 associate with mitochondrial DNA depletion syndromes which severely affect muscle and brain tissue. Dysfunction within succinate-CoA ligase disrupts the energy production process leading to cellular energy deficits. Additionally this protein’s interaction with SUCLG2 implies potential implications in similar disorders related to mitochondrial function showing the importance of their combined activity in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.

See full target information SUCLG1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:13812-13831 PubMed39565223

2024

Crosstalk between paralogs and isoforms influences p63-dependent regulatory element activity.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Baniulyte,Abby A McCann,Dana L Woodstock,Morgan A Sammons

The EMBO journal 43:2337-2367 PubMed38649537

2024

SUCLG1 restricts POLRMT succinylation to enhance mitochondrial biogenesis and leukemia progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Yan,Chengmei Xie,Sijun Sun,Quan Zheng,Jingyi Wang,Zihao Wang,Cheuk-Him Man,Haiyan Wang,Yunfan Yang,Tianshi Wang,Leilei Shi,Shengjie Zhang,Chen Huang,Shuangnian Xu,Yi-Ping Wang

Journal of translational medicine 21:132 PubMed36803784

2023

P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Gaohong Sheng,Yuan Gao,Qing Ding,Ruizhuo Zhang,Tianqi Wang,Shaoze Jing,Hongqi Zhao,Tian Ma,Hua Wu,Yong Yang

Brain structure & function 225:639-667 PubMed31982949

2020

Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.

Applications

Unspecified application

Species

Unspecified reactive species

Arpad Dobolyi,Attila Bago,Miklos Palkovits,Natalia S Nemeria,Frank Jordan,Judit Doczi,Attila Ambrus,Vera Adam-Vizi,Christos Chinopoulos

Journal of molecular and cellular cardiology 138:304-317 PubMed31836543

2019

Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Cole R Michel,Ying H Lin,Timothy A McKinsey,Mark Y Jeong,Amrut V Ambardekar,Joseph C Cleveland,Richard Reisdorph,Nichole Reisdorph,Kathleen C Woulfe,Kristofer S Fritz

Proteomics. Clinical applications 12:e1700165 PubMed29676857

2018

Proteomics Analysis Reveals Abnormal Electron Transport and Excessive Oxidative Stress Cause Mitochondrial Dysfunction in Placental Tissues of Early-Onset Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongwei Xu,Xiaohan Jin,Wei Cai,Maobin Zhou,Ping Shao,Zhen Yang,Rong Fu,Jin Cao,Yan Liu,Fang Yu,Rong Fan,Yan Zhang,Shuang Zou,Xin Zhou,Ning Yang,Xu Chen,Yuming Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SUCLG1 antibody

Western blot - Anti-SUCLG1 antibody (AB97867)

Western blot - Anti-SUCLG1 antibody (AB97867)

Western blot - Anti-SUCLG1 antibody (AB97867)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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