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AB317213

Anti-SUCLG1 antibody [HL2387]

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Rabbit Monoclonal SUCLG1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUCLG1.

View Alternative Names

Succinyl-CoA synthetase subunit alpha, SCS-alpha, SUCLG1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunofluorescent analysis. Sample : HeLa cells were fixed in ice-cold MeOH for 5 min. Green : SUCLG1 stained by ab317213 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded rat tissue. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG1 antibody [HL2387] (AB317213)

ab317213 detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissue. SUCLG1 stained by ab317213 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • WB

Supplier Data

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317213 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SUCLG1 antibody [HL2387] (ab317213) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • WB

Supplier Data

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317213 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SUCLG1 antibody [HL2387] (ab317213) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)
  • WB

Supplier Data

Western blot - Anti-SUCLG1 antibody [HL2387] (AB317213)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317213 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SUCLG1 antibody [HL2387] (ab317213) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2387

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SUCLG1.

P53597

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUCLG1 also known as succinate-CoA ligase (GDP-forming) subunit alpha participates in cellular energy metabolism. It catalyzes the conversion of succinyl-CoA to succinate in the mitochondria linking the citric acid cycle and oxidative phosphorylation. SUCLG1 has a molecular mass of approximately 32 kDa. This protein expresses highly in metabolically active tissues such as the liver kidney and brain where energy demand is significant.
Biological function summary

Succinate-CoA ligase facilitates the synthesis of nucleoside triphosphates within the mitochondria by participating in the GDP-specific form of the enzyme complex. SUCLG1 forms a heterodimer with SUCLG2 (the beta subunit) creating succinate-CoA ligase's functional enzyme complex. Through its action SUCLG1 directly impacts the generation of GTP which plays a critical role in modulating metabolic reactions within cells.

Pathways

Succinate-CoA ligase integrates into the citric acid cycle and plays a significant role in cellular respiration. By converting succinyl-CoA to succinate it helps sustain the cycle's continued operation. The protein also links with nucleoside diphosphate kinase which governs the maintenance of cellular nucleotide balance. These interactions highlight the essential nature of SUCLG1 in maintaining energy flow within cells.

Mutations in SUCLG1 associate with mitochondrial DNA depletion syndromes which severely affect muscle and brain tissue. Dysfunction within succinate-CoA ligase disrupts the energy production process leading to cellular energy deficits. Additionally this protein’s interaction with SUCLG2 implies potential implications in similar disorders related to mitochondrial function showing the importance of their combined activity in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.
See full target information SUCLG1
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Product promise

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For full details, please see our Terms & Conditions

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