JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241375

Anti-SUCLG2 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SUCLG2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SUCLG2 aa 350 to C-terminus.

View Alternative Names

GTP-specific succinyl-CoA synthetase subunit beta, Succinyl-CoA synthetase beta-G chain, G-SCS, GTPSCS, SCS-betaG, SUCLG2

2 Images
Western blot - Anti-SUCLG2 antibody (AB241375)
  • WB

Supplier Data

Western blot - Anti-SUCLG2 antibody (AB241375)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-SUCLG2 antibody (ab241375) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 47 kDa

true

Exposure time: 30s

Immunoprecipitation - Anti-SUCLG2 antibody (AB241375)
  • IP

Supplier Data

Immunoprecipitation - Anti-SUCLG2 antibody (AB241375)

SUCLG2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241375 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241375 at 1 μg/ml.

Lane 1 : ab241375 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-SUCLG2 antibody (ab241375)

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SUCLG2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96I99

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to SUCLG2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SUCLG2 protein also known as succinyl-CoA synthetase GDP-forming beta subunit is an essential component of cellular energy metabolism. It has an approximate molecular mass of 50 kDa. SUCLG2 is primarily expressed in various tissues including liver kidney and heart. It forms part of the succinyl-CoA synthetase enzyme complex which plays a role in the conversion of succinyl-CoA to succinate in the mitochondrial matrix.
Biological function summary

SUCLG2 contributes to the production of GTP through substrate-level phosphorylation an important step in the tricarboxylic acid (TCA) cycle. As part of the succinyl-CoA synthetase complex it aids in the intricate process of energy conversion within mitochondria. The enzymatic activity of SUCLG2 ensures the proper balance of GTP within cells influencing various metabolic processes essential for cellular function and maintenance.

Pathways

SUCLG2 operates within the TCA cycle and is a pivotal link in cellular respiration. In this pathway it ensures the conversion of carbon and energy flow important for energy production. SUCLG2's role here is closely related to the protein SUCLG1 with which it forms the functional succinyl-CoA synthetase complex. Additionally SUCLG2 participates in metabolic pathways related to the biosynthesis of amino acids further extending its influence on cellular metabolism.

SUCLG2 mutations have been implicated in mitochondrial diseases particularly those affecting energy metabolism. For instance patients with such mutations might experience metabolic myopathy syndromes. SUCLG2 also displays connections with SUCLG1 in these disorders given their role in the same enzymatic complex. Recognition of these associations helps in understanding the molecular basis of certain metabolic syndromes and potential avenues for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
See full target information SUCLG2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe

Genetics 221: PubMed35731217

2022

FMRP-dependent production of large dosage-sensitive proteins is highly conserved.

Applications

Unspecified application

Species

Unspecified reactive species

Keegan Flanagan,Alireza Baradaran-Heravi,Qi Yin,Khanh Dao Duc,Allan C Spradling,Ethan J Greenblatt
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB96172

Anti-SUCLG2 antibody

Western blot - Anti-SUCLG2 antibody (AB96172)

  • 0
  • 0
Primary Antibodies

AB187996

Anti-SUCLG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (AB187996)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB97867

Anti-SUCLG1 antibody

Western blot - Anti-SUCLG1 antibody (AB97867)

  • 0
  • 0
Primary Antibodies

AB134956

Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)

  • 5
  • 1
Primary Antibodies

AB202582

Anti-SUCLA2 antibody [EPR14924]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SUCLA2 antibody [EPR14924] (AB202582)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com