AB96172

Anti-SUCLG2 antibody

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(2 Publications)

Rabbit Polyclonal SUCLG2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUCLG2 aa 1-250.

View Alternative Names

GTP-specific succinyl-CoA synthetase subunit beta, Succinyl-CoA synthetase beta-G chain, G-SCS, GTPSCS, SCS-betaG, SUCLG2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (AB96172)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (AB96172)

Immunohistochemical analysis of paraffin-embedded hepatoma tissue labelling SUCLG2 protein at cytosol with ab96172 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunocytochemistry/ Immunofluorescence - Anti-SUCLG2 antibody (AB96172)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUCLG2 antibody (AB96172)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling SUCLG2 protein at mitochondria using ab96172 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar= 10µm.

Supplier Data

Western blot - Anti-SUCLG2 antibody (AB96172)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-SUCLG2 antibody (ab96172) at 1/3000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Unknown

Western blot - Anti-SUCLG2 antibody (AB96172)

10% SDS PAGE

All lanes:

Western blot - Anti-SUCLG2 antibody (ab96172) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa S3 whole cell lysate at 30 µg

Lane 5:

Hep G2 whole cell lysate at 30 µg

Lane 6:

MOLT4 whole cell lysate at 30 µg

Lane 7:

Raji whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-SUCLG2 antibody (AB96172)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-SUCLG2 antibody (ab96172) at 1/10000 dilution

All lanes:

Mouse liver lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SUCLG2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q96I99

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SUCLG2 protein also known as succinyl-CoA synthetase GDP-forming beta subunit is an essential component of cellular energy metabolism. It has an approximate molecular mass of 50 kDa. SUCLG2 is primarily expressed in various tissues including liver kidney and heart. It forms part of the succinyl-CoA synthetase enzyme complex which plays a role in the conversion of succinyl-CoA to succinate in the mitochondrial matrix.

Biological function summary

SUCLG2 contributes to the production of GTP through substrate-level phosphorylation an important step in the tricarboxylic acid (TCA) cycle. As part of the succinyl-CoA synthetase complex it aids in the intricate process of energy conversion within mitochondria. The enzymatic activity of SUCLG2 ensures the proper balance of GTP within cells influencing various metabolic processes essential for cellular function and maintenance.

Pathways

SUCLG2 operates within the TCA cycle and is a pivotal link in cellular respiration. In this pathway it ensures the conversion of carbon and energy flow important for energy production. SUCLG2's role here is closely related to the protein SUCLG1 with which it forms the functional succinyl-CoA synthetase complex. Additionally SUCLG2 participates in metabolic pathways related to the biosynthesis of amino acids further extending its influence on cellular metabolism.

SUCLG2 mutations have been implicated in mitochondrial diseases particularly those affecting energy metabolism. For instance patients with such mutations might experience metabolic myopathy syndromes. SUCLG2 also displays connections with SUCLG1 in these disorders given their role in the same enzymatic complex. Recognition of these associations helps in understanding the molecular basis of certain metabolic syndromes and potential avenues for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

See full target information SUCLG2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bosnian journal of basic medical sciences 20:44-55 PubMed31215856

2019

Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfei Zhang,Qian Sun,Lingling Jia,Ming Li

Brain structure & function 220:135-51 PubMed24085565

2013

Exclusive neuronal expression of SUCLA2 in the human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Arpád Dobolyi,Elsebet Ostergaard,Attila G Bagó,Tamás Dóczi,Miklós Palkovits,Aniko Gál,Mária J Molnár,Vera Adam-Vizi,Christos Chinopoulos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SUCLG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLG2 antibody (AB96172)

Immunocytochemistry/ Immunofluorescence - Anti-SUCLG2 antibody (AB96172)

Western blot - Anti-SUCLG2 antibody (AB96172)

Western blot - Anti-SUCLG2 antibody (AB96172)

Western blot - Anti-SUCLG2 antibody (AB96172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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