Anti-SUFU antibody [1783CT536.263.29] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SUFU antibody [1783CT536.263.29] - BSA and Azide free (AB302680)

This data was developed using ab302679, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the band beneath the target band is unknown. Exposure time : 3 minutes

All lanes:

Western blot - Anti-SUFU antibody [1783CT536.263.29] (<a href='/en-us/products/primary-antibodies/sufu-antibody-1783ct53626329-ab302679'>ab302679</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 53 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-SUFU antibody [1783CT536.263.29] - BSA and Azide free (AB302680)

This data was developed using ab302679, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS Exposure time : False colour image of Western blot : Anti-SUFU antibody [1783CT536.263.29] (ab302679) staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab302679 was shown to bind specifically to SUFU. A band was observed at 47 kDa in wild-type HAP1 cell lysates with no signal observed at this size in the SUFU knockout cell line. To generate this image, wild-type and SUFU knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution. Performed under reducing conditions. The identity of the bands beneath the target band are unknown.

All lanes:

Western blot - Anti-SUFU antibody [1783CT536.263.29] (<a href='/en-us/products/primary-antibodies/sufu-antibody-1783ct53626329-ab302679'>ab302679</a>) at 1/1000 dilution

Lane 1:

Wild type HAP1, whole cell lysate 20 µg

Lane 2:

SUFU knockout HAP1, whole cell lysate 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell), whole cell lysate 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Observed band size: 53 kDa

false