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Rabbit Recombinant Monoclonal SUFU antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.


Images

Western blot - Anti-SUFU antibody [EPR23821-101] (AB259975), expandable thumbnail
  • Western blot - Anti-SUFU antibody [EPR23821-101] (AB259975), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SUFU antibody [EPR23821-101] (AB259975), expandable thumbnail
  • Western blot - Anti-SUFU antibody [EPR23821-101] (AB259975), expandable thumbnail
  • Western blot - Anti-SUFU antibody [EPR23821-101] (AB259975), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Expected
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

We recommend using fresh lysate to improve band intensity. Protein should be transfered to membrane immediately to minimize protein degradation.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:12068298, PubMed:12975309, PubMed:15367681, PubMed:22365972, PubMed:24217340, PubMed:24311597, PubMed:27234298, PubMed:28965847). Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24217340, PubMed:24311597). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24217340, PubMed:24311597). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340). Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling (By similarity). Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PubMed:24311597, PubMed:28965847). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PubMed:24311597, PubMed:28965847). Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PubMed:24311597, PubMed:28965847). When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PubMed:24311597, PubMed:28965847). Required for normal embryonic development (By similarity). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity).

Alternative names

UNQ650/PRO1280, SUFU, Suppressor of fused homolog, SUFUH

Recommended products

Rabbit Recombinant Monoclonal SUFU antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23821-101
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SUFU also known as Suppressor of Fused is a regulatory protein with a mass of approximately 54 kDa. This protein serves as a significant component within the Hedgehog signaling pathway. SUFU is widely expressed in various tissues including brain heart and skeletal muscle. It acts as a negative regulator binding to and inhibiting the activity of other key proteins in the signaling cascade.

Biological function summary

SUFU modulates the activity of the transcription factors Gli1 Gli2 and Gli3 keeping them in the cytoplasm in their inactive form. It can form part of large protein complexes playing an important role in ensuring that signal transduction is tightly regulated. SUFU's function helps control the expression of target genes that are important for development and cellular differentiation. This regulation is essential during embryonic development and tissue maintenance in adults.

Pathways

SUFU has an important role in the Hedgehog signaling pathway and interacts with components like Smoothened (SMO) and Patched (PTCH). This pathway controls processes like cell growth and differentiation. By regulating the activity of Gli proteins SUFU influences how signals are transmitted within the pathway. SUFU can also interact with other proteins such as KIF7 further defining its place within the pathway's network.

Associated diseases and disorders

SUFU can contribute to the development of basal cell carcinoma and medulloblastoma when its function is disrupted. In cases where mutations affect SUFU regulation the Hedgehog pathway becomes aberrantly activated. This can lead to uncontrolled cell proliferation. SUFU's relationship with Gli proteins is significant in these conditions because of its role in modulating their activity directly impacting the onset and progression of these cancers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lanes 1-3: Merged signal (red and green). Green - ab259975 observed at 53 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 Anti-SUFU antibody [EPR23821-101] was shown to specifically react with SUFU in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SUFU knockout HEK-293T cell line ab267282 (knockout cell lysate Human SUFU knockout HEK-293T cell lysate ab257718) was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1: Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: SUFU knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lanes 1-3: Merged signal (red and green). Green - ab259975 observed at 53 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 Anti-SUFU antibody [EPR23821-101] was shown to specifically react with SUFU in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SUFU knockout HEK-293T cell line ab267282 (knockout cell lysate Human SUFU knockout HEK-293T cell lysate ab257718) was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

    Anti-KDM6A / UTX antibody [EPR23203-211] ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1: Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: SUFU knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: Western blot - Human SUFU knockout HEK-293T cell line (Human SUFU knockout HEK-293T cell line ab267282)

    Lane 3: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Flow Cytometry (Intracellular) - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line, Right) / SUFU KO HAP1 (Left) cells labelling SUFU with ab259975 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 37 seconds.

    All lanes: Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    All lanes: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975), expandable thumbnail

    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

    SUFU Western blot staining using rabbit Anti-SUFU antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend using fresh lysate to improve band intensity. Protein in lysate should be transferred to membrane immediately to minimize protein degradation.

    Lanes 1 - 2: Western blot - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lanes 1 and 3: Frozen 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lanes 2 and 4: Fresh (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 54 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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