Knockout Tested Rabbit Recombinant Monoclonal SUFU antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img445634.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705)")
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img407306.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705)")
![Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--immunoprecipitation-img407342.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705)")
![Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--immunoprecipitation-img407341.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705)")
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img407307.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (AB307705)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt (Intra) | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes We recommend using fresh lysate to improve band intensity. Protein should be transfered to membrane immediately to minimize protein degradation. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:12068298, PubMed:12975309, PubMed:15367681, PubMed:22365972, PubMed:24217340, PubMed:24311597, PubMed:27234298, PubMed:28965847). Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24217340, PubMed:24311597). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24217340, PubMed:24311597). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340). Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling (By similarity). Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PubMed:24311597, PubMed:28965847). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PubMed:24311597, PubMed:28965847). Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PubMed:24311597, PubMed:28965847). When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PubMed:24311597, PubMed:28965847). Required for normal embryonic development (By similarity). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity).
Alternative names
UNQ650/PRO1280, SUFU, Suppressor of fused homolog, SUFUH
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal SUFU antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26229-17
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUFU also known as Suppressor of Fused is a regulatory protein with a mass of approximately 54 kDa. This protein serves as a significant component within the Hedgehog signaling pathway. SUFU is widely expressed in various tissues including brain heart and skeletal muscle. It acts as a negative regulator binding to and inhibiting the activity of other key proteins in the signaling cascade.
- Biological function summary
SUFU modulates the activity of the transcription factors Gli1 Gli2 and Gli3 keeping them in the cytoplasm in their inactive form. It can form part of large protein complexes playing an important role in ensuring that signal transduction is tightly regulated. SUFU's function helps control the expression of target genes that are important for development and cellular differentiation. This regulation is essential during embryonic development and tissue maintenance in adults.
- Pathways
SUFU has an important role in the Hedgehog signaling pathway and interacts with components like Smoothened (SMO) and Patched (PTCH). This pathway controls processes like cell growth and differentiation. By regulating the activity of Gli proteins SUFU influences how signals are transmitted within the pathway. SUFU can also interact with other proteins such as KIF7 further defining its place within the pathway's network.
- Associated diseases and disorders
SUFU can contribute to the development of basal cell carcinoma and medulloblastoma when its function is disrupted. In cases where mutations affect SUFU regulation the Hedgehog pathway becomes aberrantly activated. This can lead to uncontrolled cell proliferation. SUFU's relationship with Gli proteins is significant in these conditions because of its role in modulating their activity directly impacting the onset and progression of these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img445634.jpg "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)")
Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)
SUFU Western blot staining using rabbit Anti-SUFU antibody
This data was developed using Anti-SUFU antibody [EPR26229-17] ab307704, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
We recommend using fresh lysate to improve band intensity. Protein in lysate should be transferred to membrane immediately to minimize protein degradation.
Anti-SUFU antibody [EPR23821-101] ab259975 shows higher sensitivity than this product.
Lanes 1 - 2: Western blot - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution
Lanes 3 - 4: Western blot - Anti-SUFU antibody [EPR23821-101] (Anti-SUFU antibody [EPR23821-101] ab259975) at 1/1000 dilution
Lanes 1 and 3: Frozen 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lanes 2 and 4: Fresh (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa
Exposure time: 180s
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img407306.jpg "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)")
Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)
This data was developed using Anti-SUFU antibody [EPR26229-17] ab307704, the same antibody clone in a different buffer formulation.
Blocking and dilution concentration: 5% NFDM/TBST.
In Western blot, Anti-SUFU antibody [EPR26229-17] ab307704 was shown to bind specifically to SUFU. A band was observed at 54 kDa in wild-type 293T cell lysates whereas no signal observed at this size in SUFU knockout cell line Human SUFU knockout HEK-293T cell line ab267282 (knockout cell lysate Human SUFU knockout HEK-293T cell lysate ab257718). Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution
Lane 1: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: SUFU knockout 293T whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot at 1/200000 dilution
Developed using the ECL technique.
Observed band size: 54 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--immunoprecipitation-img407342.jpg "Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)")
Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)
This data was developed using Anti-SUFU antibody [EPR26229-17] ab307704, the same antibody clone in a different buffer formulation.
SUFU was immunoprecipitated from 0.35 mg Mouse testis tissue lysate 10 ug with Anti-SUFU antibody [EPR26229-17] ab307704 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SUFU antibody [EPR26229-17] ab307704 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10 ug
Lane 2: Anti-SUFU antibody [EPR26229-17] ab307704 IP in Mouse testis tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SUFU antibody [EPR26229-17] ab307704 in mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 67 secondsAll lanes: Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution
All lanes: Mouse testis cells
SecondaryAll lanes: Immunoprecipitation at 1/5000 dilution
Observed band size: 54 kDa
Exposure time: 67s
![Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--immunoprecipitation-img407341.jpg "Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)")
Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)
All lanes: Immunoprecipitation - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution
All lanes: 293T cells
SecondaryAll lanes: Immunoprecipitation at 1/5000 dilution
Observed band size: 54 kDa
Exposure time: 41s
![Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705), expandable thumbnail](https://content.abcam.com/products/images/sufu-antibody-epr26229-17-bsa-and-azide-free-ab307705--western-blot-img407307.jpg "Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)")
Western blot - Anti-SUFU antibody [EPR26229-17] - BSA and Azide free (ab307705)
This data was developed using Anti-SUFU antibody [EPR26229-17] ab307704, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The Lane 1 was developed using a high sensitivity ECL substrate.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure: 180 secondsAll lanes: Western blot - Anti-SUFU antibody [EPR26229-17] (Anti-SUFU antibody [EPR26229-17] ab307704) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot at 1/200000 dilution
Developed using the ECL technique.
Observed band size: 54 kDa
Exposure time: 180s
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