Rabbit Polyclonal SUGT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SUGT1 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SUGT1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2Z0
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Alternative names
Protein SGT1 homolog, Protein 40-6-3, Sgt1, Suppressor of G2 allele of SKP1 homolog, SUGT1
Recommended products
Rabbit Polyclonal SUGT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SUGT1 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SUGT1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2Z0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUGT1 also known as Suppressor of G2 allele of SKP1 homolog is a protein widely recognized for its role in cellular processes. It has a mass of approximately 43 kDa. Researchers typically find SUGT1 expressed in a variety of tissues with significant levels noted in the brain and reproductive organs. Its presence indicates key functions in diverse cellular environments contributing to its significance in biological research.
- Biological function summary
In cellular operations SUGT1 works as a co-chaperone with HSP90 forming complexes that stabilize proteins and assist in protein folding. This interaction is critical for maintaining the proper functioning of proteins like kinases and transcription factors. SUGT1's role in forming protein complexes shows its influence in cellular stability and stress responses which helps cells combat various stresses they encounter.
- Pathways
Researchers place SUGT1 within important frameworks like the Wnt signaling and kinetochore assembly pathways. In the Wnt pathway SUGT1 modulates the activity of other proteins such as beta-catenin which play roles in cell cycle regulation and embryonic development. SUGT1 associates with proteins like CCT and HSP90 via these pathways guiding processes critical for normal cell function and division.
- Associated diseases and disorders
SUGT1 involvement becomes noticeable in cancer and neurodegenerative diseases. In certain cancers altered SUGT1 activity can affect the stabilization of proteins involved in cell growth potentially leading to unchecked cell proliferation. Moreover its connection with proteins such as p53 demonstrates its role in pathways linked to cancer progression. Additionally in neurodegenerative diseases the interaction between SUGT1 and proteins like tau indicates its potential role in the pathophysiology of disorders like Alzheimer's disease highlighting the importance of understanding its functions to explore therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUGT1 antibody (ab204428)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labeling SUGT1 with ab204428 at 1/50 dilution.
Western blot - Anti-SUGT1 antibody (ab204428)
All lanes: Western blot - Anti-SUGT1 antibody (ab204428) at 1/100 dilution
Lane 1: RT-4 lysate
Lane 2: U-251 MG lysate
Lane 3: Human plasma lysate
Lane 4: Human liver lysate
Predicted band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SUGT1 antibody (ab204428)
Immunofluorescent analysis of formalin fixed, triton X-100 permealized A431 cells labeling SUGT1 with ab204428 at 4 μg/ml (green).
Downloads
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