AB32763

Anti-Sulfatase 1/SULF1 antibody

Name: Anti-Sulfatase 1/SULF1 antibody (ab32763) | Abcam
Brand: Abcam Limited
SKU: AB32763
Price: 490 USD
Availability: InStock
Rating: 4 (5 reviews)

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What is this?

(5 Reviews)

(11 Publications)

Rabbit Polyclonal Sulfatase 1/SULF1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications.

View Alternative Names

KIAA1077, SULF1, Extracellular sulfatase Sulf-1, hSulf-1, Arylsulfatase, N-acetylglucosamine-6-sulfatase

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (AB32763)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (AB32763)

IHC image of Sulfatase 1/SULF1 staining in Human skeletal muscle formalin-fixed paraffin embedded tissue section*, performed on a Leica Bond™ system.. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32763, 0.1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-0.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Quail": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfatase 1 also known as SULF1 or HSulf-1 is an enzyme that functions by removing sulfate groups from heparan sulfate proteoglycans. Its molecular mass is approximately 125 kDa. SULF1 is mainly expressed in the liver and it is also found in the kidney thyroid and skin tissues. It possesses a sulfatase activity that acts on the 6-O-position of heparan sulfate thereby modulating the binding sites for several growth factors.

Biological function summary

SULF1 plays a significant role in modulating cellular processes like growth angiogenesis and tissue repair by altering the bioavailability of heparan sulfate-bound signaling molecules. It often acts as a monomer but may participate in larger complexes during functional modulation. SULF1 regulates interactions with key growth factors such as fibroblast growth factors (FGFs) and vascular endothelial growth factors (VEGFs) impacting cell communication and migration.

Pathways

SULF1 is an important regulator in signaling pathways including the VEGF signaling pathway and the FGF signaling pathway. These pathways are essential for processes like angiogenesis and embryonic development. SULF1 activity influences these pathways by modifying heparan sulfate chains affecting the binding of FGFs and VEGFs to their respective receptors alongside related proteins like SULF2 which shares similar functions in these pathways.

SULF1's modulation of cellular signaling makes it relevant in cancer and fibrosis where aberrant signaling contributes to disease progression. Altered SULF1 expression has been associated with certain carcinomas such as hepatocellular carcinoma where it can influence tumor growth and metastasis. Additionally SULF1 interacts with proteins like matrix metalloproteinases (MMPs) which are involved in the degradation of extracellular matrix components in both tumor progression and fibrotic conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity (PubMed : 12368295, PubMed : 12686563). It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin (PubMed : 12368295, PubMed : 12686563). Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation (PubMed : 12686563).

See full target information SULF1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 116:2774-2787 PubMed40706632

2025

Single-Cell Transcription Reveals the Fibroblast Heterogeneity and Neural Cells' Significance in Desmoid Fibromatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Wang,Cheng Zhou,Peng Huang,Haodong Li,Ping Xu,Yiming Zheng,Dahui Wang,Bo Ning

eLife 12: PubMed36622753

2023

EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Shin Lin,Chiao-Chen Chung,Yu-Chia Liu,Chu-Han Chang,Hui-Chia Liu,Yung-Yi Liang,Teng-Le Huang,Tsung-Ming Chen,Che-Hsin Lee,Chih-Hsin Tang,Mien-Chie Hung,Ya-Huey Chen

Arteriosclerosis, thrombosis, and vascular biology 41:1408-1427 PubMed33626908

2021

Single-Cell Transcriptomic Atlas of Different Human Cardiac Arteries Identifies Cell Types Associated With Vascular Physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Hu,Wendao Liu,Xiumeng Hua,Xiao Chen,Yuan Chang,Yiqing Hu,Zhenyu Xu,Jiangping Song

International journal of oncology 57:223-236 PubMed32377705

2020

HSulf‑1 and palbociclib exert synergistic antitumor effects on RB‑positive triple‑negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fengxia Chen,Zhicai Zhang,Yihan Yu,Qiuyu Liu,Feifei Pu

Toxicology and applied pharmacology 348:43-53 PubMed29673857

2018

Crystalline silica alters Sulfatase-1 expression in rat lungs which influences hyper-proliferative and fibrogenic effects in human lung epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy N Perkins,Paul M Peeters,Catrin Albrecht,Roel P F Schins,Mieke A Dentener,Brooke T Mossman,Emiel F M Wouters,Niki L Reynaert

Scientific reports 7:13847 PubMed29062064

2017

Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Okada,Kazuko Keino-Masu,Satoshi Nagamine,Fuyuki Kametani,Tatsuyuki Ohto,Masato Hasegawa,Toin H van Kuppevelt,Satoshi Kunita,Satoru Takahashi,Masayuki Masu

Journal of neurochemistry 142:534-544 PubMed28547849

2017

Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche.

Applications

Unspecified application

Species

Unspecified reactive species

Taihei Yamada,Aurelien Kerever,Yusuke Yoshimura,Yuji Suzuki,Risa Nonaka,Kyohei Higashi,Toshihiko Toida,Frederic Mercier,Eri Arikawa-Hirasawa

PloS one 9:e93711 PubMed24690739

2014

Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis.

Applications

Species

Human

Vino T Cheriyan,Ying Wang,Magesh Muthu,Shazia Jamal,Di Chen,Huanjie Yang,Lisa A Polin,Adi L Tarca,Harvey I Pass,Q Ping Dou,Sunita Sharma,Anil Wali,Arun K Rishi

PloS one 6:e23274 PubMed21853101

2011

hSulf-1 gene exhibits anticancer efficacy through negatively regulating VEGFR-2 signaling in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Weidan Ji,Jiahe Yang,Duanming Wang,Lu Cao,Weifeng Tan,Haihua Qian,Bin Sun,Qijun Qian,Zhengfeng Yin,Mengchao Wu,Changqing Su

Proceedings of the National Academy of Sciences of 107:10202-7 PubMed20479257

2010

Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways.

Applications

Species

Human

Shuhei Otsuki,Sarah R Hanson,Shigeru Miyaki,Shawn P Grogan,Mitsuo Kinoshita,Hiroshi Asahara,Chi-Huey Wong,Martin K Lotz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sulfatase 1/SULF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (AB32763)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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