AB232835

Anti-Sulfatase 2/SULF2 antibody

Name: Anti-Sulfatase 2/SULF2 antibody (ab232835) | Abcam
Brand: Abcam Limited
SKU: AB232835
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Sulfatase 2/SULF2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SULF2 aa 300-650.

View Alternative Names

KIAA1247, UNQ559/PRO1120, SULF2, Extracellular sulfatase Sulf-2, hSulf-2, Arylsulfatase, N-acetylglucosamine-6-sulfatase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Sulfatase 2/SULF2 antibody (AB232835)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sulfatase 2/SULF2 antibody (AB232835)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Sulfatase 2/SULF2 using ab232835 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody (AB232835)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody (AB232835)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Sulfatase 2/SULF2 using ab232835 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Sulfatase 2/SULF2 antibody (AB232835)

All lanes:

Western blot - Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/mL

All lanes:

Recombinant human Sulfatase 2/SULF2 protein

Predicted band size: 100 kDa

false

Supplier Data

Western blot - Anti-Sulfatase 2/SULF2 antibody (AB232835)

All lanes:

Western blot - Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/mL

All lanes:

Pig liver lysate

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SULF2 aa 300-650. The exact immunogen used to generate this antibody is proprietary information.

Q8IWU5

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfatase 2 (SULF2) also known as SULF-2 or extracellular sulfatase acts as an enzyme that removes sulfate groups from the heparan sulfate proteoglycans (HSPGs). It is a 100-110 kDa protein located primarily on the cell surface. SULF2's expression occurs mainly in tissues such as the liver kidney and lungs although it can be found in various other tissues too. By modifying HSPGs SULF2 influences cell signaling events that are critical to cellular communication and function.

Biological function summary

SULF2 participates in the modulation of growth factor signaling and cell surface interactions. This enzyme does not exist in isolation; instead it functions as a part of complexes involved in the extracellular matrix (ECM). Through its catalytic action SULF2 modulates the availability and activity of signaling molecules such as fibroblast growth factors (FGFs) and Wnt proteins. It impacts processes such as cell proliferation differentiation and developmental processes by altering the structural properties of the ECM.

Pathways

SULF2 significantly modulates the Wnt signaling pathway and the fibroblast growth factor receptor (FGFR) pathway. These pathways play essential roles in development and tissue regeneration. By altering the sulfation states of heparan sulfate chains SULF2 affects the interaction of proteins like Frizzled receptors in the Wnt pathway and FGFRs in the FGF signaling pathway. This modulation leads to changes in signal transduction further influencing the cellular responses during growth and development.

SULF2 has been linked with several cancer types and neurodegenerative diseases. In cancer such as hepatocellular carcinoma SULF2 overexpression promotes tumor growth and invasion by influencing growth factor signaling. It often associates with proteins like β-catenin in cancer-related pathways. In neurodegenerative diseases SULF2 modulates neural stem cell differentiation and repair influencing conditions like Alzheimer's disease. The enzyme's interaction with amyloid-beta peptides and tau protein can impact disease progression and severity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity (PubMed : 12368295, PubMed : 30788513, PubMed : 35294879). It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin (PubMed : 12368295, PubMed : 30788513, PubMed : 35294879).

See full target information SULF2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 26:2483989 PubMed40140347

2025

Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine M Manouchehri,Jharna Datta,Lynn M Marcho,Daniel Stover,Ramesh K Ganju,Bhuvaneswari Ramaswamy,William E Carson,Arjun Mittra,Xiaoli Zhang,Patrick M Schnell,Yu Yue,Mark P Rubinstein,Mathew A Cherian

Frontiers in oncology 12:808889 PubMed35433465

2022

Chemotherapeutic Risk lncRNA-PVT1 SNP Sensitizes Metastatic Colorectal Cancer to FOLFOX Regimen.

Applications

Unspecified application

Species

Unspecified reactive species

Shenshen Wu,Xi Yang,Weiyan Tang,Giuseppe Familiari,Michela Relucenti,Michael Aschner,Xiaobo Li,Rui Chen

Cell cycle (Georgetown, Tex.) 18:1727-1744 PubMed31204561

2019

MicroRNA-422a functions as a tumor suppressor in non-small cell lung cancer through SULF2-mediated TGF-β/SMAD signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Qiang Li,Jian-Peng Zhang,Yan-Yu Wang,Xin-Zhen Li,Lin Sun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sulfatase 2/SULF2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Sulfatase 2/SULF2 antibody (AB232835)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody (AB232835)

Western blot - Anti-Sulfatase 2/SULF2 antibody (AB232835)

Western blot - Anti-Sulfatase 2/SULF2 antibody (AB232835)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Product promise