AB129094

Anti-Sulfite oxidase antibody [EPR7618]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Sulfite oxidase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

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3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

ab129094 at a 1/50 dilution staining Sulfite oxidase in paraffin embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

All lanes:

Western blot - Anti-Sulfite oxidase antibody [EPR7618] (ab129094) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

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OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

OI-RD Scanning

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OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7618

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfite oxidase also known as SUOX has a molecular mass of approximately 57 kDa and plays a critical role in the conversion of sulfite to sulfate. This enzyme is located in the mitochondria of cells across various tissues but is prominently expressed in the liver and kidney. Its activity depends on molybdenum and heme cofactors rendering it an important player in cellular redox reactions.

Biological function summary

Sulfite oxidase acts in the detoxification process of sulfur-containing amino acids. It functions as a monomer and ensures that the potentially harmful intermediate sulfite is efficiently converted to the harmless end product sulfate which can then be excreted. This detoxification is important for cellular health and prevents accumulation of sulfite which could lead to oxidative damage.

Pathways

Sulfite oxidase plays an important part in two major metabolic processes: the sulfur amino acid detoxification pathway and the metabolic pathway of the degradation of branched-chain amino acids. It works together with cytochrome c in the electron transport chain to facilitate electron transfer. Sulfite oxidase’s function ensures proper catabolism of amino acids and assists in maintaining cellular homeostasis.

Sulfite oxidase deficiency can cause severe neurological impairment and developmental delay often linked to sulfite oxidase deficiency disorder itself. This genetic disorder results from mutations in the SUOX gene disrupting proper enzyme function. Associations have also been highlighted with neurological conditions where oxidative stress plays a part implicating this enzyme’s failure to properly regulate sulfite levels. Additionally its interactions with the molybdenum cofactor highlight connections to molybdenum cofactor deficiency further illustrating its importance in human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation of sulfur-containing amino acids.

See full target information SUOX

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:458 PubMed39560723

2024

NFκB and JNK pathways mediate metabolic adaptation upon ESCRT-I deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Jaroslaw Cendrowski,Marta Wrobel,Michal Mazur,Bartosz Jary,Ranjana Maurya,Surui Wang,Michal Korostynski,Anna Dziewulska,Maria Rohm,Patryk Kuropka,Natalia Pudelko-Malik,Piotr Mlynarz,Agnieszka Dobrzyn,Anja Zeigerer,Marta Miaczynska

Journal of inherited metabolic disease 45:169-182 PubMed34741542

2021

A defect in molybdenum cofactor binding causes an attenuated form of sulfite oxidase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Tobias Kaczmarek,Daniel Bender,Titus Gehling,Joshua Benedict Kohl,Hülya-Sevcan Daimagüler,José Angel Santamaria-Araujo,Max Christoph Liebau,Anne Koy,Sebahattin Cirak,Guenter Schwarz

Redox biology 38:101800 PubMed33271457

2020

The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and HS metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Theresa Mellis,Albert L Misko,Sita Arjune,Ye Liang,Katalin Erdélyi,Tamás Ditrói,Alexander T Kaczmarek,Peter Nagy,Guenter Schwarz

Journal of inherited metabolic disease 43:748-757 PubMed31950508

2020

Oxygen and nitrite reduction by heme-deficient sulphite oxidase in a patient with mild sulphite oxidase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Bender,Alexander T Kaczmarek,Sabina Kuester,Alberto B Burlina,Guenter Schwarz

Laboratory investigation; a journal of technical m 94:557-68 PubMed24589856

2014

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Ekman,Bengt Uvelius,Sebastian Albinsson,Karl Swärd

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sulfite oxidase antibody [EPR7618]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

Western blot - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] (AB129094)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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