JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248296

Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Sulfite oxidase antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SUOX

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)

This data was developed using ab129094, the same antibody clone in a different buffer formulation.

ab129094, at a 1/50 dilution, staining Sulfite oxidase in paraffin embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)
  • WB

Unknown

Western blot - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)

This data was developed using ab129094, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sulfite oxidase antibody [EPR7618] (<a href='/en-us/products/primary-antibodies/sulfite-oxidase-antibody-epr7618-ab129094'>ab129094</a>) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] - BSA and Azide free (AB248296)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7618

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab248296 is the carrier-free version of ab129094.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfite oxidase also known as SUOX has a molecular mass of approximately 57 kDa and plays a critical role in the conversion of sulfite to sulfate. This enzyme is located in the mitochondria of cells across various tissues but is prominently expressed in the liver and kidney. Its activity depends on molybdenum and heme cofactors rendering it an important player in cellular redox reactions.
Biological function summary

Sulfite oxidase acts in the detoxification process of sulfur-containing amino acids. It functions as a monomer and ensures that the potentially harmful intermediate sulfite is efficiently converted to the harmless end product sulfate which can then be excreted. This detoxification is important for cellular health and prevents accumulation of sulfite which could lead to oxidative damage.

Pathways

Sulfite oxidase plays an important part in two major metabolic processes: the sulfur amino acid detoxification pathway and the metabolic pathway of the degradation of branched-chain amino acids. It works together with cytochrome c in the electron transport chain to facilitate electron transfer. Sulfite oxidase’s function ensures proper catabolism of amino acids and assists in maintaining cellular homeostasis.

Sulfite oxidase deficiency can cause severe neurological impairment and developmental delay often linked to sulfite oxidase deficiency disorder itself. This genetic disorder results from mutations in the SUOX gene disrupting proper enzyme function. Associations have also been highlighted with neurological conditions where oxidative stress plays a part implicating this enzyme’s failure to properly regulate sulfite levels. Additionally its interactions with the molybdenum cofactor highlight connections to molybdenum cofactor deficiency further illustrating its importance in human health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation of sulfur-containing amino acids.
See full target information SUOX
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB166625

Anti-TST antibody [EPR11646(B)]

RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB174302

Anti-ETHE1 antibody [EPR11697]

RabMAb|Recombinant

Western blot - Anti-ETHE1 antibody [EPR11697] (AB174302)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com