AB243069

Anti-Sulfotyrosine antibody [12B11]

Name: Anti-Sulfotyrosine antibody [12B11] (ab243069) | Abcam
Brand: Abcam Limited
SKU: AB243069
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Sulfotyrosine antibody. Suitable for WB, IHC-P and reacts with Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Sulfotyrosine.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [12B11] (AB243069)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Sulfotyrosine using ab243069 at 1/25 dilution for 1 hour at room temperature in immunohistochemical analysis (C). The nuclear counterstain is DAPI (blue, A). Actin is detected with Actin-binding Phalloidin-AlexaFluor^® 633 (red, B). (D) is a composite image.

Secondary Antibody : Goat Anti-Mouse IgG : AlexaFluor^® 488.

Unknown

Western blot - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Sulfotyrosine antibody [12B11] (ab243069) at 1/1000 dilution

Lane 1:

Sulfotyrosine-BSA at 2 µg

Lane 2:

Tyrosine-BSA at 2 µg

Lane 3:

Phosphotyrosine-BSA at 2 µg

Lane 4:

BSA at 2 µg

Secondary

Lanes 1 - 3:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

Lane 4:

Goat Anti-Mouse IgG at 1/2000 dilution

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12B11

Isotype

IgG1

Carrier free

Applications

IHC-P, WB

applications

Specificity

Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25", "IHCP-species-notes": "<p></p>" } } }

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Product details

The clone number has been updated from 7C5 to 12B11, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfotyrosine is a post-translational modification of the amino acid tyrosine where a sulfate group gets added to the phenolic hydroxyl moiety. This process is known as tyrosine sulfation and plays a critical role in protein-protein interactions. Tyrosine sulfation occurs primarily in the Golgi apparatus and is mediated by tyrosylprotein sulfotransferases. It doesn't refer to a single protein but rather the chemical modification found on various proteins. The distinct molecular mass for the modification itself is about 80 Da taking into account the addition of the sulfate group. This modification mostly appears in secretory and membrane proteins across a wide range of tissues and organisms.

Biological function summary

The sulfotyrosine modification significantly enhances the binding properties of proteins involved in diverse biological processes. It affects proteins such as adhesion molecules G protein-coupled receptors and coagulation factors often being essential for their proper function. Many proteins containing sulfotyrosine participate in forming multi-protein complexes that mediate cell signaling and communication.

Pathways

Sulfotyrosine modifications integrate notably into signal transduction and immune response pathways. Proteins like P-selectin glycoprotein ligand-1 (PSGL-1) and chemokine receptors such as CCR5 require sulfotyrosine for optimal receptor-ligand interactions which impacts leukocyte trafficking and chemotaxis. These modifications often serve as docking sites for other proteins coordinating complex pathways important for cellular responses.

Sulfotyrosine modifications have implications in inflammatory diseases and cancer. The modification plays a role in mediating the interactions involving proteins like PSGL-1 in immune response pathways which are critical in inflammatory diseases such as rheumatoid arthritis. Additionally abnormal tyrosine sulfation on chemokine receptors like CCR5 has associations with the metastatic properties of cancer influencing both tumor development and immune evasion mechanisms. Their correlation emphasizes the importance of understanding sulfotyrosine's functional roles in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sulfotyrosine

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1617185 PubMed40926987

2025

Renoprotective effects of GHRH agonist MR409 is associated with reduced oxidative stress and ferroptosis in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Liu,Rong Fu,Qi Tang,Yaoxia Zhang,Ruiping Cai,Limin Liu,Hui Jia,Junjia Gao,Ming-Sheng Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sulfotyrosine antibody [12B11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Western blot - Anti-Sulfotyrosine antibody [12B11] (AB243069)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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