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AB136481

Anti-Sulfotyrosine antibody [Sulfo-1C-A2]

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(4 Publications)

Mouse Monoclonal Sulfotyrosine antibody. Suitable for WB, IP, cELISA and reacts with Modified Amino Acid samples. Cited in 4 publications.
2 Images
Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)
  • WB

Unknown

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)

Lanes 1 - 3:

control IgG

Lanes 4 - 6:

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (ab136481) at 1/1000 dilution

Lanes 1 and 4:

3T3/NIH cell lysate

Lanes 2 and 5:

HEK 293 cell lysate

Lanes 3 and 6:

Bovine cardiac tissue lysate

Secondary

All lanes:

Goat anti-mouse secondary antibody conjugated to HRP at 1/2000 dilution

true

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)
  • WB

Unknown

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (AB136481)

All lanes:

Western blot - Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (ab136481) at 1/1000 dilution

Lane 1:

Purified bovine fibrinogen treated with 1.5 mg/ml abalone sulfatase at 37°C overnight.

Lane 2:

Purified bovine fibrinogen

Secondary

All lanes:

Goat anti-mouse secondary antibody conjugated to HRP at 1/2000 dilution

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Sulfo-1C-A2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Applications

cELISA, IP, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "cELISA-species-checked": "guaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.5 Preservative: 0.05% Sodium azide Constituents: 1.35% Glycine, 1.21% Tris, 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulfotyrosine is a post-translational modification of the amino acid tyrosine where a sulfate group gets added to the phenolic hydroxyl moiety. This process is known as tyrosine sulfation and plays a critical role in protein-protein interactions. Tyrosine sulfation occurs primarily in the Golgi apparatus and is mediated by tyrosylprotein sulfotransferases. It doesn't refer to a single protein but rather the chemical modification found on various proteins. The distinct molecular mass for the modification itself is about 80 Da taking into account the addition of the sulfate group. This modification mostly appears in secretory and membrane proteins across a wide range of tissues and organisms.
Biological function summary

The sulfotyrosine modification significantly enhances the binding properties of proteins involved in diverse biological processes. It affects proteins such as adhesion molecules G protein-coupled receptors and coagulation factors often being essential for their proper function. Many proteins containing sulfotyrosine participate in forming multi-protein complexes that mediate cell signaling and communication.

Pathways

Sulfotyrosine modifications integrate notably into signal transduction and immune response pathways. Proteins like P-selectin glycoprotein ligand-1 (PSGL-1) and chemokine receptors such as CCR5 require sulfotyrosine for optimal receptor-ligand interactions which impacts leukocyte trafficking and chemotaxis. These modifications often serve as docking sites for other proteins coordinating complex pathways important for cellular responses.

Sulfotyrosine modifications have implications in inflammatory diseases and cancer. The modification plays a role in mediating the interactions involving proteins like PSGL-1 in immune response pathways which are critical in inflammatory diseases such as rheumatoid arthritis. Additionally abnormal tyrosine sulfation on chemokine receptors like CCR5 has associations with the metastatic properties of cancer influencing both tumor development and immune evasion mechanisms. Their correlation emphasizes the importance of understanding sulfotyrosine's functional roles in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Sulfotyrosine
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 23:155 PubMed39095793

2024

Genome-wide CRISPR screening identifies tyrosylprotein sulfotransferase-2 as a target for augmenting anti-PD1 efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Yumi Oh,Sujeong Kim,Yunjae Kim,Hyun Kim,Dongjun Jang,Seungjae Shin,Soo-Jin Lee,Jiwon Kim,Sang Eun Lee,Jaeik Oh,Yoojin Yang,Dohee Kim,Hae Rim Jung,Sangjin Kim,Jihui Kim,Kyungchan Min,Beomki Cho,Hoseok Seo,Dohyun Han,Hansoo Park,Sung-Yup Cho

Scientific reports 10:19818 PubMed33188246

2020

Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis.

Applications

Unspecified application

Species

Unspecified reactive species

Doreen A Wüstenhagen,Phil Lukas,Christian Müller,Simone A Aubele,Jan-Peter Hildebrandt,Stefan Kubick

The Journal of biological chemistry 294:11087-11100 PubMed31152061

2019

Differential cleavage of lysyl oxidase by the metalloproteinases BMP1 and ADAMTS2/14 regulates collagen binding through a tyrosine sulfate domain.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Rosell-García,Alberto Paradela,Gema Bravo,Laura Dupont,Mourad Bekhouche,Alain Colige,Fernando Rodriguez-Pascual

Molecular & cellular proteomics : MCP 17:2480-2495 PubMed30097533

2018

Discovery of a Human Testis-specific Protein Complex TEX101-DPEP3 and Selection of Its Disrupting Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Schiza,Dimitrios Korbakis,Efstratia Panteleli,Keith Jarvi,Andrei P Drabovich,Eleftherios P Diamandis
View all publications
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