Anti-SULT2A1/ST2 antibody [EPR16096]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1/ST2 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

• mIHC

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Multiplex immunohistochemistry - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; red; Opal™690) at 1 : 2000 (0.263 μg/ml) [Panel B], anti-C11B2/CYP11B2 stained on zona glomerulosa (ab249476; cyan; Opal™520) at 1 : 500 (4.478 μg/ml) [Panel C], and anti-SULT2A1 stained on zona reticularis (ab240333; yellow; Opal™570) at 1 : 500 (4.504 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab274418, ab249476, and ab240333 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. This data was developed using ab240333, the same antibody clone in a different buffer formulation.

• WB

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Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Lane 3:

Human adrenal gland lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

• WB

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Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Mouse SULT2A1 has gender difference and is only expressed in female (PMID : 28455387, PMID : 31201272, PMID : 16807285).

All lanes:

Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Predicted band size: 34 kDa

Observed band size: 35 kDa

false

Exposure time: 60s

• WB

Supplier Data

Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/2000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Rat kidney lysate at 10 µg

Lane 3:

Rat liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false