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Rabbit Recombinant Monoclonal SULT2A1/ST2 antibody. Suitable for mIHC, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113), expandable thumbnail
  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113), expandable thumbnail
  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (AB194113), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/120
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups (PubMed:14573603, PubMed:18042734, PubMed:19589875, PubMed:21187059, PubMed:2268288, PubMed:29671343, PubMed:7678732, PubMed:7854148). Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis (PubMed:14573603, PubMed:19589875, PubMed:21187059). Plays a key role in bile acid metabolism (PubMed:2268288). In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols (By similarity).

Alternative names

HST, STD, SULT2A1, Sulfotransferase 2A1, ST2A1, Bile salt sulfotransferase, Dehydroepiandrosterone sulfotransferase, Hydroxysteroid Sulfotransferase, ST2, SULT2A3, DHEA-ST, DHEA-ST8

Recommended products

Rabbit Recombinant Monoclonal SULT2A1/ST2 antibody. Suitable for mIHC, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16096
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SULT2A1 also known as ST2 is an enzyme belonging to the sulfotransferase family. It mechanically transfers sulfate groups to hydroxy steroids which include substances like dehydroepiandrosterone (DHEA) as well as other sterols. SULT2A1 exhibits an approximate molecular mass of 34 kDa. Expression occurs mainly in the liver and adrenal glands where it plays important role in steroid metabolism. It is also found in the intestine and skin although at lower levels. This enzyme's function in these tissues suggests its involvement in the local regulation of hormone action and metabolism.

Biological function summary

The role of SULT2A1 extends to the regulation of hormones by sulfurylation which makes compounds more soluble and ready for excretion. This process effectively deactivates certain steroid hormones thereby modulating their activity within the body. SULT2A1 does not typically form part of a larger complex but works independently in modifying steroid substrates. The enzyme's activity allows for regulation of hormone availability and subsequently holds substantial influence over multiple physiological processes.

Pathways

SULT2A1 is active in the steroid metabolism and catabolic pathways. It functions alongside other enzymes like CYP3A4 a cytochrome P450 enzyme to transform and clear hormone derivatives from the body. This cooperation is important for maintaining hormonal balance by converting active hormones into less active forms or preparing them for elimination. SULT2A1 contributes specifically to the homeostasis of androgen and estrogen metabolism through the sulfate conjugation pathway regulating the sensitivity of tissues to these hormones.

Associated diseases and disorders

Alterations in SULT2A1 activity have connections with adrenal diseases and certain forms of cancer. Overactive or deficient SULT2A1 enzyme function can disrupt hormone levels affecting conditions like congenital adrenal hyperplasia. This condition involves hormone imbalances due to enzyme irregularities upstream where SULT2A1's role in sulfate conjugation remains integral. It is also associated with breast cancer where enzyme interactions with estrogen derivatives might modulate proliferative signals; related proteins include estrogen receptor pathways that influence tumor growth. Understanding SULT2A1's function in these contexts aids in grasping the broader impact of hormone regulation and metabolism in disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1/ST2 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: Human fetal liver lysate at 20 µg

    Lane 3: Human adrenal gland lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/2000 dilution

    Lane 1: Mouse spleen lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Lane 3: Rat liver lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Mouse SULT2A1 has gender difference and is only expressed in female (PMID: 28455387, PMID: 31201272, PMID: 16807285).

    All lanes: Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Rat liver tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse spleen tissue lysate at 20 µg

    Predicted band size: 34 kDa

    Observed band size: 35 kDa

    Exposure time: 60s

  • Multiplex immunohistochemistry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (Anti-PCSK2 antibody [EPR23578-19] ab274418; red; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-C11B2/CYP11B2 stained on zona glomerulosa (Anti-C11B2/CYP11B2 antibody [EPR10494] - BSA and Azide free ab249476; cyan; Opal™520) at 1:500 (4.478 μg/ml) [Panel C], and anti-SULT2A1 stained on zona reticularis (Anti-SULT2A1/ST2 antibody [EPR16096] - BSA and Azide free ab240333; yellow; Opal™570) at 1:500 (4.504 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of Anti-PCSK2 antibody [EPR23578-19] ab274418, Anti-C11B2/CYP11B2 antibody [EPR10494] - BSA and Azide free ab249476, and Anti-SULT2A1/ST2 antibody [EPR16096] - BSA and Azide free ab240333 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    This data was developed using Anti-SULT2A1/ST2 antibody [EPR16096] - BSA and Azide free ab240333, the same antibody clone in a different buffer formulation.

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Product protocols

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