Rabbit Recombinant Monoclonal SULT2B1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SULT2B1 aa 1 to C-terminus.
Images
![Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055), expandable thumbnail](https://content.abcam.com/products/images/sult2b1-antibody-007-bsa-and-azide-free-ab276055--western-blot-img8546.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)")
![Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055), expandable thumbnail](https://content.abcam.com/products/images/sult2b1-antibody-007-bsa-and-azide-free-ab276055--immunoprecipitation-img20166.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human SULT2B1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00204
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 0.2-1 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Target data
Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (PubMed:12145317, PubMed:19589875). Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA) (PubMed:12145317, PubMed:16855051, PubMed:21855633, PubMed:9799594). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA (PubMed:12145317, PubMed:21855633). Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed:28575648). Isoform 2. Sulfonates pregnenolone but not cholesterol.
Alternative names
HSST2, SULT2B1, Sulfotransferase 2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, Sulfotransferase family 2B member 1, Sulfotransferase family cytosolic 2B member 1, ST2B1
Recommended products
Rabbit Recombinant Monoclonal SULT2B1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SULT2B1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human SULT2B1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00204
- Carrier free
- Yes
- Clone number
- 007
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SULT2B1 also known as sulfotransferase family 2B member 1 functions as a cytosolic enzyme that adds sulfate groups to various molecules. This post-translational modification is important for the metabolism of hormones neurotransmitters drugs and xenobiotic compounds. SULT2B1 has a molecular mass of approximately 42 kDa. It is expressed in a variety of tissues with higher levels in the skin brain and reproductive organs indicating its diverse physiological roles across different systems in the body.
- Biological function summary
The role of SULT2B1 involves the sulfation of cholesterol and dehydroepiandrosterone (DHEA) which are important for maintaining lipid homeostasis and steroid hormone biosynthesis. The enzyme is not part of a complex but works optimally in its standalone cytosolic environment. It participates actively in regulating the availability of active hormones and lipids making it a significant player in cellular metabolic processes.
- Pathways
SULT2B1 operates within two main biological frameworks: the steroid hormone biosynthesis pathway and lipid metabolic pathway. In these pathways the enzyme interacts closely with cholesterol and DHEA to modulate their sulfation status influencing precursor availability for steroidogenesis. It works in conjunction with other sulfotransferases like SULT1A1 that also help manage the sulfate pool important for hormone regulation and metabolic balance.
- Associated diseases and disorders
Aberrations in SULT2B1 function can link to conditions like polycystic ovary syndrome (PCOS) and certain cancers due to its role in steroid hormone metabolism. Disruptions in the enzyme's activity could alter DHEA and cholesterol sulfation leading to hormone imbalances that contribute to these disorders. In the context of PCOS associated enzymes might include subtypes of cytochrome P450 which function alongside SULT2B1 in steroid biosynthesis pathways to maintain hormone equilibrium.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055), expandable thumbnail](https://content.abcam.com/products/images/sult2b1-antibody-007-bsa-and-azide-free-ab276055--western-blot-img8546.jpg "Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)")
Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)
Developed using the Odyssey technique.
Performed under reducing conditions.All lanes: Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055) at 1/500 dilution
All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 41 kDa
![Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055), expandable thumbnail](https://content.abcam.com/products/images/sult2b1-antibody-007-bsa-and-azide-free-ab276055--immunoprecipitation-img20166.jpg "Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)")
Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)
SULT2B1 was immunoprecipitated from 0.5 mg of MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 0.5 μL ab276055 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276055 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.All lanes: Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)
Predicted band size: 41 kDa
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