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AB276055

Anti-SULT2B1 antibody [007] - BSA and Azide free

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Rabbit Recombinant Monoclonal SULT2B1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SULT2B1 aa 1-400.

View Alternative Names

HSST2, SULT2B1, Sulfotransferase 2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, Sulfotransferase family 2B member 1, Sulfotransferase family cytosolic 2B member 1, ST2B1

2 Images
Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)
  • IP

Unknown

Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)

SULT2B1 was immunoprecipitated from 0.5 mg of MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 0.5 μL ab276055 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276055 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.
Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055)

Predicted band size: 41 kDa

false

Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)
  • WB

Unknown

Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (AB276055)

Developed using the Odyssey technique.

Performed under reducing conditions.

All lanes:

Western blot - Anti-SULT2B1 antibody [007] - BSA and Azide free (ab276055) at 1/500 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

007

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human SULT2B1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

O00204

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.2-1 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SULT2B1 also known as sulfotransferase family 2B member 1 functions as a cytosolic enzyme that adds sulfate groups to various molecules. This post-translational modification is important for the metabolism of hormones neurotransmitters drugs and xenobiotic compounds. SULT2B1 has a molecular mass of approximately 42 kDa. It is expressed in a variety of tissues with higher levels in the skin brain and reproductive organs indicating its diverse physiological roles across different systems in the body.
Biological function summary

The role of SULT2B1 involves the sulfation of cholesterol and dehydroepiandrosterone (DHEA) which are important for maintaining lipid homeostasis and steroid hormone biosynthesis. The enzyme is not part of a complex but works optimally in its standalone cytosolic environment. It participates actively in regulating the availability of active hormones and lipids making it a significant player in cellular metabolic processes.

Pathways

SULT2B1 operates within two main biological frameworks: the steroid hormone biosynthesis pathway and lipid metabolic pathway. In these pathways the enzyme interacts closely with cholesterol and DHEA to modulate their sulfation status influencing precursor availability for steroidogenesis. It works in conjunction with other sulfotransferases like SULT1A1 that also help manage the sulfate pool important for hormone regulation and metabolic balance.

Aberrations in SULT2B1 function can link to conditions like polycystic ovary syndrome (PCOS) and certain cancers due to its role in steroid hormone metabolism. Disruptions in the enzyme's activity could alter DHEA and cholesterol sulfation leading to hormone imbalances that contribute to these disorders. In the context of PCOS associated enzymes might include subtypes of cytochrome P450 which function alongside SULT2B1 in steroid biosynthesis pathways to maintain hormone equilibrium.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (PubMed : 12145317, PubMed : 19589875). Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA) (PubMed : 12145317, PubMed : 16855051, PubMed : 21855633, PubMed : 9799594). Preferentially sulfonates cholesterol, while it also has significant activity with pregnenolone and DHEA (PubMed : 12145317, PubMed : 21855633). Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed : 28575648).. Isoform 2. Sulfonates pregnenolone but not cholesterol.
See full target information SULT2B1
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Product promise

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