Rabbit Polyclonal Sumo 1 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 34 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO1.
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- Science advances 9:eadl21082023RAD54L2 counters TOP2-DNA adducts to promote genome stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppina D'Alessandro,David A Morales-Juarez,Sean L Richards,Karin C Nitiss,Almudena Serrano-Benitez,Juanjuan Wang,John C Thomas,Vipul Gupta,Andrea Voigt,Rimma Belotserkovskaya,Chen Gang Goh,Anne Ramsay Bowden,Yaron Galanty,Petra Beli,John L Nitiss,Guido Zagnoli-Vieira,Stephen P JacksonPubMed 38055822
- Cell death & disease 14:1472023Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQindong Guo,Yang Fan,Qingtong Wang,Boyan Li,Wei Qiu,Yanhua Qi,Ziwen Pan,Shouji Zhang,Shulin Zhao,Kehui Yang,Hao Xu,Ming Li,Zijie Gao,Jianye Xu,Huizhi Wang,Shaobo Wang,Qilin Tang,Jiawei Qiu,Xing Guo,Lin Deng,Ping Zhang,Rongrong Zhao,Hao Xue,Chengwei Wang,Gang LiPubMed 36810326
- Oxidative medicine and cellular longevity 2022:80025662022Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Yu,Xiyun Bian,Chunyan Zhang,Zhouying Wu,Na Huang,Jie Yang,Wen Jin,Zongqi Feng,Dongfang Li,Xue Huo,Ting Wu,Zhongmin Jiang,Xiaozhi Liu,Dejun SunPubMed 35707278
- Cell reports 37:1100802021The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.Applications:WB, IFReactive species:HumanAnnamaria Ruggiano,Bruno Vaz,Susan Kilgas,Marta Popović,Gonzalo Rodriguez-Berriguete,Abhay N Singh,Geoff S Higgins,Anne E Kiltie,Kristijan RamadanPubMed 34879279
- Pharmacological research 172:1058432021Rg3 promotes the SUMOylation of SERCA2a and corrects cardiac dysfunction in heart failure.Applications:WBReactive species:MouseZhihao Liu,Xiyun Bian,Wenbo Gao,Jing Su,Chuanrui Ma,Xiaolin Xiao,Tian Yu,Han Zhang,Xiaozhi Liu,Guanwei FanPubMed 34428586
- Biomedical and environmental sciences : BES 34:124-1292021Astragaloside IV Improves Angiogenesis and Promotes Wound Healing in Diabetic Rats the Activation of the SUMOylation Pathway.Applications:ICC, WBReactive species:HumanBao Shen Wang,Xiao Fang Ma,Chun Yan Zhang,Yan Xia Li,Xiao Zhi Liu,Chang Qing HuPubMed 33685571
- Molecular medicine reports 23:2021Astragaloside IV improves angiogenesis under hypoxic conditions by enhancing hypoxia‑inducible factor‑1α SUMOylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaoshen Wang,Chunyan Zhang,Dongmei Chu,Xiaofang Ma,Tian Yu,Xiaozhi Liu,Changqing HuPubMed 33537820
- JCI insight 5:2020Increased SUMO-activating enzyme SAE1/UBA2 promotes glycolysis and pathogenic behavior of rheumatoid fibroblast-like synoviocytes.
- Molecular medicine reports 22:2617-26262020Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes.
- International journal of oncology 57:780-7902020Dexamethasone inhibits stemness maintenance and enhances chemosensitivity of hepatocellular carcinoma stem cells by inducing deSUMOylation of HIF‑1α and Oct4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SUMO1. Database link P63165
Reactivity data
Select an application| IHC-P | IP | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
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Target data
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).
Alternative names
SMT3C, SMT3H3, UBL1, OK/SW-cl.43, SUMO1, Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GMP1, Smt3C
Recommended products
Rabbit Polyclonal Sumo 1 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 34 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SUMO1. Database link P63165
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUMO 1 also known as small ubiquitin-like modifier 1 is part of the protein family involved in post-translational modification. The protein mechanically adds a SUMO group to target proteins through sumoylation a process similar to ubiquitination. SUMO 1 typically has a molecular mass of around 11 kDa. It expresses in various tissues and cells including the nucleus and cytoplasm of eukaryotic cells. In addition it is important in processes like nuclear transport transcriptional regulation and protein stabilization.
- Biological function summary
SUMO 1 contributes significantly to maintaining cellular homeostasis. It is an integral part of a SUMOylation complex that modifies other proteins to alter their function localization or interactions. Through its modification actions SUMO 1 affects processes such as DNA repair and the cell cycle. By interacting with components of the nuclear pore complex and transcription factors SUMO 1 modulates essential biological activities at multiple levels within the cell.
- Pathways
This protein plays a significant role in the PI3K/Akt pathway and Wnt signaling. SUMO 1 associates with proteins like RanGAP1 and various transcription factors highlighting the complexity of its regulatory actions. These associations are important for the regulation of cell survival proliferation and differentiation. SUMOylation by SUMO 1 has been linked to an interference with phosphorylation showing its potential influence on cell signaling pathways.
- Associated diseases and disorders
The dysregulation of SUMO 1 is seen in cancer and neurodegenerative diseases. For instance altered SUMOylation patterns contribute to the progression of certain cancers. Furthermore SUMO 1 interacts with proteins like p53 and these interactions can affect tumor suppression and cell cycle regulation. In neurodegenerative disorders disturbed SUMOylation is associated with protein aggregation and neuronal damage implicating SUMO 1 in diseases like Alzheimer's.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
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Immunoprecipitation - Anti-Sumo 1 antibody (ab11672)
293T cells were transfected with a vector that has Sumo1 fused to GFP and a Flag tag. Cell lysates were used in IP with ab11672 (and a GFP antibody as a control). The resulting western blot was performed with a Flag antibody. As a control, cells were transfected with a a vector with Sumo2 fused to GFP and a Flag tag. ab11672 does not IP anything from this lysate.
Lane 1: Sumo1 fusion lysate - IP'd with GFP antibody
Lane 2: Sumo1 fusion lysate - no IP
Lane 3: Sumo1 fusion lysate - IP'd with ab11672
Lane 4: Sumo2 fusion lysate - IP'd with ab11672
All lanes: Immunoprecipitation - Anti-Sumo 1 antibody (ab11672)
Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody (ab11672)
ab11672 staining human normal placenta. Staining is localized to the nucleus and nuclear membrane.
Left panel: with primary whole serum antibody at 1/400. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be
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