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Rabbit Recombinant Monoclonal Sumo 1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.


Images

Western blot - Anti-Sumo 1 antibody [EP298] (AB133352), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (AB133352), expandable thumbnail
  • Western blot - Anti-Sumo 1 antibody [EP298] (AB133352), expandable thumbnail
  • Western blot - Anti-Sumo 1 antibody [EP298] (AB133352), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (AB133352), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Rat
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Sumo 1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP298
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SUMO 1 also known as small ubiquitin-like modifier 1 is part of the protein family involved in post-translational modification. The protein mechanically adds a SUMO group to target proteins through sumoylation a process similar to ubiquitination. SUMO 1 typically has a molecular mass of around 11 kDa. It expresses in various tissues and cells including the nucleus and cytoplasm of eukaryotic cells. In addition it is important in processes like nuclear transport transcriptional regulation and protein stabilization.

Biological function summary

SUMO 1 contributes significantly to maintaining cellular homeostasis. It is an integral part of a SUMOylation complex that modifies other proteins to alter their function localization or interactions. Through its modification actions SUMO 1 affects processes such as DNA repair and the cell cycle. By interacting with components of the nuclear pore complex and transcription factors SUMO 1 modulates essential biological activities at multiple levels within the cell.

Pathways

This protein plays a significant role in the PI3K/Akt pathway and Wnt signaling. SUMO 1 associates with proteins like RanGAP1 and various transcription factors highlighting the complexity of its regulatory actions. These associations are important for the regulation of cell survival proliferation and differentiation. SUMOylation by SUMO 1 has been linked to an interference with phosphorylation showing its potential influence on cell signaling pathways.

Associated diseases and disorders

The dysregulation of SUMO 1 is seen in cancer and neurodegenerative diseases. For instance altered SUMOylation patterns contribute to the progression of certain cancers. Furthermore SUMO 1 interacts with proteins like p53 and these interactions can affect tumor suppression and cell cycle regulation. In neurodegenerative disorders disturbed SUMOylation is associated with protein aggregation and neuronal damage implicating SUMO 1 in diseases like Alzheimer's.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Sumo 1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: MCF-7 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133352 observed at 16 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab133352 was shown to recognize Sumo 1 when Sumo 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. ab133352 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

    Predicted band size: 11 kDa

    Observed band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical staining of paraffin-embedded human thyroid carcinoma sections labelling Sumo 1 with purified ab133352 at dilution of 1:100. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

    Blocking and diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-Sumo 1 antibody [EP298] (ab133352) at 1/10000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: MCF-7 (human breast carcinoma) whole cell lysate at 20 µg

    Lane 3: A549 (human lung carcinoma) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

    Lane 5: C6 (rat glioma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 11 kDa

    Observed band size: 18-90 kDa

  • Western blot - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

    All lanes: Western blot - Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HL60 cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: NIH3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical analysis of paraffin-embedded Human papillary cell thyroid gland carcinoma tissue labelling Sumo 1 with un-purified ab133352 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human Sumo 1 protein (Recombinant Human Sumo 1 protein ab140417) at 0.01 µg

    Lane 2: Western blot - Recombinant Human Sumo 2 protein (Recombinant Human Sumo 2 protein ab140420) at 0.01 µg

    Lane 3: Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

    Lane 4: Western blot - Recombinant Human Sumo 4 protein (Recombinant Human Sumo 4 protein ab157025) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa

    Observed band size: 16 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical analysis of paraffin embedded Human Glioma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

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