AB233222

Anti-Sumo 2 antibody

Name: Anti-Sumo 2 antibody (ab233222) | Abcam
Brand: Abcam Limited
SKU: AB233222
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Sumo 2 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO2.

View Alternative Names

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 antibody (AB233222)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 antibody (AB233222)

Formalin-fixed, paraffin-embedded human lung tissue stained for Sumo 2 using ab233222 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Unknown

Western blot - Anti-Sumo 2 antibody (AB233222)

All lanes:

Western blot - Anti-Sumo 2 antibody (ab233222) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-Sumo 2 antibody (AB233222)

All lanes:

Western blot - Anti-Sumo 2 antibody (ab233222) at 1 µg/mL

All lanes:

Pig cerebrum lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 11 kDa

false

Supplier Data

Western blot - Anti-Sumo 2 antibody (AB233222)

All lanes:

Western blot - Anti-Sumo 2 antibody (ab233222) at 1 µg/mL

All lanes:

Pig liver lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 11 kDa

false

Supplier Data

Western blot - Anti-Sumo 2 antibody (AB233222)

All lanes:

Western blot - Anti-Sumo 2 antibody (ab233222) at 2 µg/mL

All lanes:

Recombinant human Sumo 2 protein

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SUMO2.

P61956

Specificity

Due to high homology between Sumo 2 and Sumo 3, cross-reactivity of ab233222 with Sumo 3 may be observed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sumo 2 also known as SUMO-2 or SMT3A is a small ubiquitin-like modifier protein with a molecular mass of approximately 11 kDa. It belongs to the SUMO protein family which includes SUMO-1 SUMO-3 and others. Sumo 2 is widely expressed in the nucleus and cytoplasm of eukaryotic cells. It is involved in the post-translational modification process known as SUMOylation where it covalently attaches to specific lysine residues on substrate proteins influencing their activity localization and stability.

Biological function summary

Sumo 2 influences various cellular processes including nuclear-cytosolic transport transcriptional regulation and protein stability. It often forms part of multi-protein complexes showing versatility in regulating cellular responses to stress and maintaining protein homeostasis. Sumo 2's dynamic attachment and detachment from its target proteins allow fine-tuning of cellular pathways reflecting its essential role in managing cellular conditions.

Pathways

Sumo 2 participates in the DNA damage response and the heat shock response pathways. Its involvement helps ensure proper cell cycle progression and stress response through interaction with key proteins like p53 and HSF1. Sumo 2 modification alters the activity of these proteins and others affecting cell survival under stress conditions and facilitating DNA repair mechanisms showing its comprehensive integration in these important cellular processes.

Alterations in Sumo 2 function have links to neurodegenerative diseases and cancer. Abnormal SUMOylation patterns involving Sumo 2 correlate with the progression of Alzheimer's disease and various types of tumors. In these contexts Sumo 2 modulates proteins such as tau and MDM2 which are critical in disease development and progression. Exploring the role of Sumo 2 in these conditions highlights its potential as a target for therapeutic intervention and diagnostic applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).

See full target information SUMO2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38203469

2024

RanBP2/Nup358 Mediates Sumoylation of STAT1 and Antagonizes Interferon-α-Mediated Antiviral Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Li,Lili Su,Jing Jiang,Yifan E Wang,Yingying Ling,Yi Qiu,Huahui Yu,Yucong Huang,Jiangmin Wu,Shan Jiang,Tao Zhang,Alexander F Palazzo,Qingtang Shen

Aging 14:8032-8045 PubMed36227136

2022

SENP3 affects the expression of PYCR1 to promote bladder cancer proliferation and EMT transformation by deSUMOylation of STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Jian Liu,Huifeng Fu,Yuanwei Li,Qiang Liu,Wei Song,Mingqiang Zeng

Technology in cancer research & treatment 20:15330338211019376 PubMed34032148

2021

LncRNA SNHG3 Promotes Proliferation and Metastasis of Non-Small-Cell Lung Cancer Cells Through miR-515-5p/SUMO2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqun Li,Lipin Gao,Caiyun Zhang,Jiguang Meng

Gland surgery 10:1067-1084 PubMed33842251

2021

An immunohistochemical panel of three small ubiquitin-like modifier genes predicts outcomes of patients with triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Lin,Qingshui Wang,Yingying Lin,Meichen Jiang,Han Xiao,Jie Zhang,Rongrong Guo,Shaohong Kang,Yao Lin,Chuangui Song

The Journal of clinical investigation 131: PubMed33661764

2021

SUMOylation promotes extracellular vesicle-mediated transmission of lncRNA ELNAT1 and lymph node metastasis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Changhao Chen,Hanhao Zheng,Yuming Luo,Yao Kong,Mingjie An,Yuting Li,Wang He,Bowen Gao,Yue Zhao,Hao Huang,Jian Huang,Tianxin Lin

Metabolism: clinical and experimental 100:153954 PubMed31400386

2019

The metabolic regulator small heterodimer partner contributes to the glucose and lipid homeostasis abnormalities induced by hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jizheng Chen,Yue Zhou,Yuan Zhuang,Tian Qin,Min Guo,Jing Jiang,Junqi Niu,John Zhong Li,Xinwen Chen,Qian Wang

PloS one 14:e0217578 PubMed31125383

2019

Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency.

Applications

Unspecified application

Species

Unspecified reactive species

Gretchen L Bentz,Angela J Lowrey,Dustin C Horne,Vy Nguyen,Austin R Satterfield,Tabithia D Ross,Abigail E Harrod,Olga N Uchakina,Robert J McKallip

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sumo 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 antibody (AB233222)

Western blot - Anti-Sumo 2 antibody (AB233222)

Western blot - Anti-Sumo 2 antibody (AB233222)

Western blot - Anti-Sumo 2 antibody (AB233222)

Western blot - Anti-Sumo 2 antibody (AB233222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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