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Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 is a mouse monoclonal antibody that is used in Sumo 2 + Sumo 3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone 8A2 has been tried and trusted by researchers since 2009 and is cited in >60 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (AB81371), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).

Targets

SUMO3

Alternative names

Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SUMO2, SMT3H2, SMT3B

Recommended products

Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 is a mouse monoclonal antibody that is used in Sumo 2 + Sumo 3 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone 8A2 has been tried and trusted by researchers since 2009 and is cited in >60 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

8A2

Purification technique

Affinity purification Protein A

Specificity

This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product has switched from ascites to recombinant production method on 25th August 2020.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Associated diseases and disorders

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276 at 10 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

    Lane 4: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 5: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

    Lane 6: 293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Lane 8: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

    Predicted band size: 12 kDa

    Observed band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with ab81371 for overnight at 4°C.
    Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Dilution and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 10 µg

    Lane 2: Human kidney tissue lysate at 10 µg

    Lane 3: Human spleen tissue lysate at 10 µg

    Lane 4: Mouse brain tissue lysate at 10 µg

    Lane 5: Rat spleen tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 12 kDa

    Observed band size: 15 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with ab81371 for overnight at 4°C.
    Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)

    Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

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