Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free

• ICC

Supplier Data

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• ICC

Supplier Data

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control : Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control : Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

• ICC

Supplier Data

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control : Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

• WB

Supplier Data

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 10 µg

Lane 2:

Human kidney tissue lysate at 10 µg

Lane 3:

Human spleen tissue lysate at 10 µg

Lane 4:

Mouse brain tissue lysate at 10 µg

Lane 5:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276 at 10 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 6:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Predicted band size: 12 kDa

false