Skip to main content

Mouse Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805), expandable thumbnail
  • Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (AB252805), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).

Additional Targets

SUMO3

Alternative names

Recommended products

Mouse Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
8A2
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab252805 is the carrier-free version of Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Associated diseases and disorders

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com