Mouse Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Mouse, Rat, Human samples.
Constituents: 100% PBS
IHC-P | ICC | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Tested |
Rat | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).
SUMO3
SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B
Mouse Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Mouse, Rat, Human samples.
Constituents: 100% PBS
This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.
ab252805 is the carrier-free version of Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.
Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.
Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.
Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276 at 10 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg
Lane 4: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 5: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg
Lane 6: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 8: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)
Predicted band size: 12 kDa
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 10 µg
Lane 2: Human kidney tissue lysate at 10 µg
Lane 3: Human spleen tissue lysate at 10 µg
Lane 4: Mouse brain tissue lysate at 10 µg
Lane 5: Rat spleen tissue lysate at 10 µg
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 12 kDa
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
This image was produced using Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371, the same antibody clone in a different buffer.
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with Anti-Sumo 2 + Sumo 3 antibody [8A2] ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
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