AB282157

Anti-Sumo 2 + Sumo 3 antibody [AT10F1]

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal Sumo 2 antibody. Suitable for ICC/IF, WB, Flow Cyt and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUMO2.

View Alternative Names

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SMT3A, SMT3H1, SUMO3, Small ubiquitin-related modifier 3, SUMO-3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3A, Smt3A

4 Images

Flow Cytometry - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Flow cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labelling SUMO2/3 with ab282157 at 2-5ug for 1x10⁶cells (red). A Goat anti mouse IgG (Alexa Fluor^® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SUMO2/3 with ab282157 at 1/100 dilution followed by Alexa Fluor^® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).

Supplier Data

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

SDS-PAGE gel was transferred to PVDF membrane.

ECL detection system.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 40 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 40 µg

Lane 4:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 40 µg

Secondary

All lanes:

Goat anti-Mouse secondary antibody HRP-conjugate

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

SDS-PAGE gel was transferred to PVDF membrane.

ECL detection system.

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157) at 1/1000 dilution

Lane 1:

Recombinant human SUMO1 protein at 0.001 µg

Lane 2:

Recombinant human SUMO2 protein at 0.001 µg

Lane 3:

Recombinant human SUMO3 protein at 0.001 µg

Secondary

All lanes:

Goat anti-Mouse secondary antibody HRP-conjugate

Predicted band size: 12 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT10F1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SUMO2.

P61956

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Sodium chloride, 0.13% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).

See full target information SUMO2

Additional targets

SUMO3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40603105

2025

KLF16-Induced Increase of GARS Promotes Glycolysis in Hepatocellular Carcinoma by Stabilizing FOXK1.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Wang,Ruijuan Fan,Jun Li,Hanbo Qiu,Lizhu Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sumo 2 + Sumo 3 antibody [AT10F1]

Flow Cytometry - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Western blot - Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (AB282157)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise