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Rabbit Recombinant Monoclonal Sumo 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Tested
Mouse
Predicted
Not recommended
Not recommended
Expected
Predicted
Rat
Predicted
Not recommended
Not recommended
Expected
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).

Additional Targets

SUMO3

Alternative names

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B

Recommended products

Rabbit Recombinant Monoclonal Sumo 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4602
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Associated diseases and disorders

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)

    Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005), expandable thumbnail

    Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)

    All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 4: SW480 cell lysate at 10 µg

    Lane 5: HL60 cell lysate at 10 µg

    Predicted band size: 12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)

    ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)

    ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005), expandable thumbnail

    Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human Sumo 1 protein (Recombinant Human Sumo 1 protein ab140417) at 0.01 µg

    Lane 2: Western blot - Recombinant Human Sumo 2 protein (Recombinant Human Sumo 2 protein ab140420) at 0.01 µg

    Lane 3: Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

    Lane 4: Western blot - Recombinant Human Sumo 4 protein (Recombinant Human Sumo 4 protein ab157025) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa

    Observed band size: 16 kDa

    Exposure time: 20s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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