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Rabbit Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (AB232032), expandable thumbnail
  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (AB232032), expandable thumbnail
  • OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (AB232032), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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    1 products for Alternative Product

      Target data

      Function

      Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).

      Alternative names

      Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SUMO2, SMT3H2, SMT3B

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      Rabbit Recombinant Monoclonal Sumo 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

      Alternative names

      Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SUMO2, SMT3H2, SMT3B

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR7163

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab232032 is the carrier-free version of Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      SUMO (Small Ubiquitin-like Modifier) proteins specifically SUMO-2 SUMO-3 and SUMO-4 are small proteins involved in modifying other proteins post-translationally. SUMO-2 and SUMO-3 are nearly identical while SUMO-4 shows some distinct differences. These proteins with an average mass of around 11 kDa covalently attach to lysine residues on target proteins a process known as SUMOylation. Expression of these proteins is widespread throughout eukaryotic cells with high expression levels in the brain heart and liver. SUMO-2 and SUMO-3 often function interchangeably forming poly-SUMO chains unlike SUMO-1.

      Biological function summary

      Through the attachment to target proteins these SUMO proteins influence various cellular processes like nuclear-cytosolic transport transcriptional regulation and DNA repair. SUMO-2 and SUMO-3 often act as part of a complex with other SUMO proteins enhancing their functional diversity. SUMOylation can alter protein-protein interactions and stabilize protein structure impacting gene expression and cellular response to stress. SUMO-4 due to a unique proline residue shows distinct regulatory functions particularly in stress responses and immune reactions.

      Pathways

      Protein SUMOylation plays an important role in major signaling pathways such as the NF-kB and p53 pathways. SUMO-2 and SUMO-3 modification can regulate the activity of p53 an important transcription factor in cell cycle control and apoptosis. Their association with the NF-kB pathway influences inflammatory responses. Importantly these modifications can affect proteins like IκBα and MDM2 which are directly involved in regulating NF-kB and p53 activities showing their involvement in maintaining cellular homeostasis and stress responses.

      Associated diseases and disorders

      Dysregulation of SUMOylation involving SUMO-2 SUMO-3 and potentially SUMO-4 is linked to cancer and neurodegenerative diseases. In cancer altered SUMO conjugation can influence cell proliferation and apoptosis affecting tumor suppressors like p53. In neurodegenerative disorders such as Alzheimer's disease impaired SUMOylation processes might contribute to the abnormal aggregation of proteins like tau. These associations highlight the significance of SUMO proteins in maintaining cellular health and their potential as therapeutic targets in these diseases.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032)

        Overlay histogram showing HEK293 cells stained with Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

        This data was developed using the same antibody clone in a different buffer formulation (Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606).

      • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032), expandable thumbnail

        Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032)

        Blocking buffer: 5% NFDM/TBST

        This data was developed using Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606, the same antibody clone in a different buffer formulation.

        All lanes: Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] ab126606) at 1/5000 dilution

        Lane 1: Western blot - Recombinant Human Sumo 1 protein (Recombinant Human Sumo 1 protein ab140417) at 0.01 µg

        Lane 2: Western blot - Recombinant Human Sumo 2 protein (Recombinant Human Sumo 2 protein ab140420) at 0.01 µg

        Lane 3: Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

        Lane 4: Western blot - Recombinant Human Sumo 4 protein (Recombinant Human Sumo 4 protein ab157025) at 0.1 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Developed using the ECL technique.

        Predicted band size: 11 kDa

        Observed band size: 16 kDa

        Exposure time: 3s

      • OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032), expandable thumbnail

        OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] - BSA and Azide free (ab232032)

        We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
        Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com