Anti-SUN1 antibody [EPR6554] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] - BSA and Azide free (AB226091)

ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124770).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] - BSA and Azide free (AB226091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124770)

• WB

Lab

Western blot - Anti-SUN1 antibody [EPR6554] - BSA and Azide free (AB226091)

This data was developed using ab124770, the same antibody clone in a different buffer formulation.

Western blot : Anti-SUN1 antibody [EPR6554] ab124770 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 105 kDa in Wild-type A549 cell lysates with a band observed at a lower molecular weight (17 kDa) in SUN1 CRISPR-Cas9 Edited A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 5:

Western blot - Anti-SUN1 antibody [EPR6554] (<a href='/en-us/products/primary-antibodies/sun1-antibody-epr6554-ab124770'>ab124770</a>) at 1/5000 dilution

Lanes 1 - 5:

Western blot - Anti-SUN1 antibody [EPR6554] - BSA and Azide free (ab226091) at 1/5000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

SUN1 CRISPR-Cas9 Edited A549 at 20 µg

Lane 3:

PANC-1 at 20 µg

Lane 4:

Raji at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 105 kDa

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