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AB327554

Anti-SUN1 antibody [HL1946] - BSA and Azide free

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Rabbit Recombinant Monoclonal SUN1 antibody. Carrier free. Suitable for ICC/IF and reacts with Human samples.
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Immunocytochemistry/ Immunofluorescence - Anti-SUN1 antibody [HL1946] - BSA and Azide free (AB327554)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUN1 antibody [HL1946] - BSA and Azide free (AB327554)

ab327554 detects SUN1 protein at nuclear envelope by immunofluorescent analysis.
Sample : A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : SUN1 stained by ab327554 diluted at 1 : 500.
Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000.
Blue : Fluoroshield with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-SUN1 antibody [HL1946] - BSA and Azide free (AB327554)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUN1 antibody [HL1946] - BSA and Azide free (AB327554)

ab327554 detects SUN1 protein at nuclear envelope by immunofluorescent analysis.
Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : SUN1 stained by ab327554 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1946

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton (PubMed : 18039933, PubMed : 18396275). The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (By similarity). Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration (By similarity). Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2 : KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly (By similarity). Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis (By similarity). Helps to define the distribution of nuclear pore complexes (NPCs) (By similarity). Required for efficient localization of SYNE4 in the nuclear envelope (By similarity). May be involved in nuclear remodeling during sperm head formation in spermatogenesis (By similarity). May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links (PubMed : 24375709).
See full target information SUN1

Alternative Names

KIAA0810, UNC84A, SUN1, SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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