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Rabbit Recombinant Monoclonal SUN2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-SUN2 antibody [EPR6556] (AB133591), expandable thumbnail
  • Western blot - Anti-SUN2 antibody [EPR6556] (AB133591), expandable thumbnail
  • OI-RD Scanning - Anti-SUN2 antibody [EPR6556] (AB133591), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.

Alternative names

FRIGG, KIAA0668, RAB5IP, UNC84B, SUN2, SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Sad1/unc-84 protein-like 2, Rab5IP

Recommended products

Rabbit Recombinant Monoclonal SUN2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6556
Purity
Tissue culture supernatant
Dissociation constant
1.01 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SUN2 also known as Sad1 and UNC84 domain containing 2 is a protein that plays a fundamental role in the positioning of the nucleus in cells. It has a mass of approximately 89 kDa. This protein localizes to the inner nuclear membrane where it is involved in tethering the nucleus to the cytoskeleton. SUN2 is mainly expressed in tissues exhibiting high mechanical stress such as muscle and bone tissue. Its structural role anchors the nuclear lamina to the cytoskeleton helping to maintain cellular integrity.

Biological function summary

SUN2 connects the nuclear interior with the cytoskeleton through the LINC complex (Linker of Nucleoskeleton and Cytoskeleton). This complex also includes other proteins like Nesprin and Lamins helping to transmit mechanical signals across the nuclear envelope. SUN2 plays an essential role in processes like cell division nuclear migration and chromosomal positioning. Its function in the LINC complex makes it a significant player in maintaining nuclear morphology and ensuring proper cell division.

Pathways

SUN2 is involved in the mechanotransduction and cell-cycle regulation pathways. In mechanotransduction SUN2 helps translate mechanical signals into biochemical ones therefore influencing gene expression. Related proteins like Nesprin-2 interact within the LINC complex and contribute to these pathways. In cell-cycle regulation the protein helps align the mitotic spindle through connection with components of the cytoskeleton supporting accurate cell division. These pathways demonstrate SUN2's contribution to cellular responses to mechanical stimuli and cell growth regulation.

Associated diseases and disorders

Studies indicate that SUN2 mutations or abnormal expression levels are associated with muscular dystrophy and cancer. In muscular dystrophy defects in SUN2 can lead to impaired nuclear anchoring contributing to muscle weakness. It interacts with Lamin A/C and defects in these proteins are also linked to muscular dystrophies. In cancer SUN2 dysregulation may affect cellular architecture and chromosomal stability contributing to tumorigenesis. Understanding SUN2's roles and interactions offers potential pathways for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-SUN2 antibody [EPR6556] (ab133591), expandable thumbnail

    Immunoprecipitation - Anti-SUN2 antibody [EPR6556] (ab133591)

    ab133591 at 1/30 dilution immunoprecipitating SUN2 in Saos-2 (human osteosarcoma) cell lysate. Total sample is 300μg. The large spread out band at 50 kDa is Rabbit IgG heavy chain.

    All lanes: Immunoprecipitation - Anti-SUN2 antibody [EPR6556] (ab133591)

    Predicted band size: 80 kDa

  • Western blot - Anti-SUN2 antibody [EPR6556] (ab133591), expandable thumbnail

    Western blot - Anti-SUN2 antibody [EPR6556] (ab133591)

    All lanes: Western blot - Anti-SUN2 antibody [EPR6556] (ab133591) at 1/1000 dilution

    Lane 1: Human fetal muscle tissue lysate at 10 µg

    Lane 2: Saos 2 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: HepG cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 80 kDa

  • OI-RD Scanning - Anti-SUN2 antibody [EPR6556] (ab133591), expandable thumbnail

    OI-RD Scanning - Anti-SUN2 antibody [EPR6556] (ab133591)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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