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AB248569

Anti-SUN2 antibody [EPR6556] - BSA and Azide free

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Rabbit Recombinant Monoclonal SUN2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

FRIGG, KIAA0668, RAB5IP, UNC84B, SUN2, SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Sad1/unc-84 protein-like 2, Rab5IP

3 Images
Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)
  • IP

Lab

Immunoprecipitation - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)

This data was developed using ab133591, the same antibody clone in a different buffer formulation.

ab133591 at 1/30 dilution immunoprecipitating SUN2 in Saos-2 (human osteosarcoma) cell lysate. Total sample is 300μg. The large spread out band at 50 kDa is Rabbit IgG heavy chain.

All lanes:

Immunoprecipitation - Anti-SUN2 antibody [EPR6556] (<a href='/en-us/products/primary-antibodies/sun2-antibody-epr6556-ab133591'>ab133591</a>)

Predicted band size: 80 kDa

false

Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)
  • WB

Unknown

Western blot - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)

This data was developed using ab133591, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SUN2 antibody [EPR6556] (<a href='/en-us/products/primary-antibodies/sun2-antibody-epr6556-ab133591'>ab133591</a>) at 1/1000 dilution

Lane 1:

Human fetal muscle tissue lysate at 10 µg

Lane 2:

Saos 2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 80 kDa

false

OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SUN2 antibody [EPR6556] - BSA and Azide free (AB248569)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6556

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248569 is the carrier-free version of ab133591.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUN2 also known as Sad1 and UNC84 domain containing 2 is a protein that plays a fundamental role in the positioning of the nucleus in cells. It has a mass of approximately 89 kDa. This protein localizes to the inner nuclear membrane where it is involved in tethering the nucleus to the cytoskeleton. SUN2 is mainly expressed in tissues exhibiting high mechanical stress such as muscle and bone tissue. Its structural role anchors the nuclear lamina to the cytoskeleton helping to maintain cellular integrity.
Biological function summary

SUN2 connects the nuclear interior with the cytoskeleton through the LINC complex (Linker of Nucleoskeleton and Cytoskeleton). This complex also includes other proteins like Nesprin and Lamins helping to transmit mechanical signals across the nuclear envelope. SUN2 plays an essential role in processes like cell division nuclear migration and chromosomal positioning. Its function in the LINC complex makes it a significant player in maintaining nuclear morphology and ensuring proper cell division.

Pathways

SUN2 is involved in the mechanotransduction and cell-cycle regulation pathways. In mechanotransduction SUN2 helps translate mechanical signals into biochemical ones therefore influencing gene expression. Related proteins like Nesprin-2 interact within the LINC complex and contribute to these pathways. In cell-cycle regulation the protein helps align the mitotic spindle through connection with components of the cytoskeleton supporting accurate cell division. These pathways demonstrate SUN2's contribution to cellular responses to mechanical stimuli and cell growth regulation.

Studies indicate that SUN2 mutations or abnormal expression levels are associated with muscular dystrophy and cancer. In muscular dystrophy defects in SUN2 can lead to impaired nuclear anchoring contributing to muscle weakness. It interacts with Lamin A/C and defects in these proteins are also linked to muscular dystrophies. In cancer SUN2 dysregulation may affect cellular architecture and chromosomal stability contributing to tumorigenesis. Understanding SUN2's roles and interactions offers potential pathways for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2 : KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.
See full target information SUN2
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Product promise

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