AB181063

Anti-SUN3 antibody [EPR12910]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SUN3 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

SUNC1, SUN3, SUN domain-containing protein 3, Sad1/unc-84 domain-containing protein 1

2 Images

Supplier Data

Western blot - Anti-SUN3 antibody [EPR12910] (AB181063)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SUN3 antibody [EPR12910] (ab181063) at 1/1000 dilution

All lanes:

LnCap cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-SUN3 antibody [EPR12910] (AB181063)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SUN3 antibody [EPR12910] (ab181063) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Carrier free

Anti-SUN3 antibody [EPR12910] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12910

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/110", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUN3 also known as Sad1 and UNC-like domain-containing protein 3 has a molecular mass of approximately 115 kDa. This protein is mechanistically involved in nuclear envelope organization and is predominantly expressed in testicular tissue specifically within spermatogenic cells. It localizes to the nuclear envelope where it is believed to contribute to the structural integrity and function of the nuclear membrane.

Biological function summary

SUN3 is involved in the formation of the LINC complex which stands for LInker of NucleOcytoskeleton Complex. This complex serves as a bridge between the nucleoskeleton and the cytoskeleton. Through this connection SUN3 assists in maintaining nuclear positioning and stability playing a role in the mechanical coupling of the nucleus with cytoskeletal elements. This interaction is essential for processes like spermatogenesis where nuclear movement and positioning are key.

Pathways

SUN3 participates in the spermatogenesis and meiotic cell division processes. The LINC complex including SUN3 partners with proteins such as KASH5 in these pathways to ensure proper chromosomal movement and alignment. Its role in these pathways is critical for normal fertility as errors can lead to abnormal sperm development and function.

Alterations in the function or expression of SUN3 affect male fertility leading to conditions such as oligozoospermia or asthenozoospermia. These conditions arise when the structural and positional integrity of the sperm nucleus is compromised. SUN3 interactions with proteins like KASH5 are significant because any disruption can result in impaired spermatogenesis contributing to these fertility issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As a probable component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nuclear remodeling during sperm head formation in spermatogenesis. A probable SUN3 : SYNE1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.

See full target information SUN3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SUN3 antibody [EPR12910]

Western blot - Anti-SUN3 antibody [EPR12910] (AB181063)

Western blot - Anti-SUN3 antibody [EPR12910] (AB181063)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Product promise