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AB250320

Anti-SUN3 antibody [EPR12910] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SUN3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SUNC1, SUN3, SUN domain-containing protein 3, Sad1/unc-84 domain-containing protein 1

2 Images
Western blot - Anti-SUN3 antibody [EPR12910] - BSA and Azide free (AB250320)
  • WB

Supplier Data

Western blot - Anti-SUN3 antibody [EPR12910] - BSA and Azide free (AB250320)

This data was developed using ab181063, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SUN3 antibody [EPR12910] (<a href='/en-us/products/primary-antibodies/sun3-antibody-epr12910-ab181063'>ab181063</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Western blot - Anti-SUN3 antibody [EPR12910] - BSA and Azide free (AB250320)
  • WB

Supplier Data

Western blot - Anti-SUN3 antibody [EPR12910] - BSA and Azide free (AB250320)

This data was developed using ab181063, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SUN3 antibody [EPR12910] (<a href='/en-us/products/primary-antibodies/sun3-antibody-epr12910-ab181063'>ab181063</a>) at 1/1000 dilution

All lanes:

LnCap cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12910

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250320 is the carrier-free version of ab181063.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUN3 also known as Sad1 and UNC-like domain-containing protein 3 has a molecular mass of approximately 115 kDa. This protein is mechanistically involved in nuclear envelope organization and is predominantly expressed in testicular tissue specifically within spermatogenic cells. It localizes to the nuclear envelope where it is believed to contribute to the structural integrity and function of the nuclear membrane.
Biological function summary

SUN3 is involved in the formation of the LINC complex which stands for LInker of NucleOcytoskeleton Complex. This complex serves as a bridge between the nucleoskeleton and the cytoskeleton. Through this connection SUN3 assists in maintaining nuclear positioning and stability playing a role in the mechanical coupling of the nucleus with cytoskeletal elements. This interaction is essential for processes like spermatogenesis where nuclear movement and positioning are key.

Pathways

SUN3 participates in the spermatogenesis and meiotic cell division processes. The LINC complex including SUN3 partners with proteins such as KASH5 in these pathways to ensure proper chromosomal movement and alignment. Its role in these pathways is critical for normal fertility as errors can lead to abnormal sperm development and function.

Alterations in the function or expression of SUN3 affect male fertility leading to conditions such as oligozoospermia or asthenozoospermia. These conditions arise when the structural and positional integrity of the sperm nucleus is compromised. SUN3 interactions with proteins like KASH5 are significant because any disruption can result in impaired spermatogenesis contributing to these fertility issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a probable component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nuclear remodeling during sperm head formation in spermatogenesis. A probable SUN3 : SYNE1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.
See full target information SUN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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