AB13499

Anti-Superoxide Dismutase 1 antibody

Name: Anti-Superoxide Dismutase 1 antibody (ab13499) | Abcam
Brand: Abcam Limited
SKU: AB13499
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal Superoxide Dismutase 1 antibody. Suitable for IHC-P, WB, ICC/IF, IP, ELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Rat Sod1.

View Alternative Names

Superoxide dismutase [Cu-Zn]

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

ab13499 staining Human normal colon. Staining is localized to the nucleus.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Superoxide Dismutase 1 with ab13499 at 1/120 dilution. Secondary antibody : FITC Goat Anti-Rabbit (green) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Superoxide Dismutase 1 with ab13499 at 1/120 dilution. Secondary antibody : APC Goat Anti-Rabbit (red) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

Unknown

Western blot - Anti-Superoxide Dismutase 1 antibody (AB13499)

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab13499) at 1 µg/mL

All lanes:

Cell lysates prepared from mixed human cell lines (A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G)

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, ELISA, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Rat Sod1. The exact immunogen used to generate this antibody is proprietary information.

P07632

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/120", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This antibody was purified on an antigen coupled sepharose column.

Storage buffer

pH: 7 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.

Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information Sod1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21638 PubMed38062139

2023

The interplay between sex, time of day, fasting status, and their impact on cardiac mitochondrial structure, function, and dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Mariame S Kane,Gloria A Benavides,Edie Osuma,Michelle S Johnson,Helen E Collins,Yecheng He,David Westbrook,Silvio H Litovsky,Kasturi Mitra,John C Chatham,Victor Darley-Usmar,Martin E Young,Jianhua Zhang

Reproductive biology and endocrinology : RB&E 17:23 PubMed30760288

2019

Activation of Nrf2/Keap1 pathway by oral Dimethylfumarate administration alleviates oxidative stress and age-associated infertility might be delayed in the mouse ovary.

Applications

Unspecified application

Species

Unspecified reactive species

Nana Akino,Osamu Wada-Hiraike,Wataru Isono,Hiromi Terao,Harunori Honjo,Yuichiro Miyamoto,Michihiro Tanikawa,Kenbun Sone,Mana Hirano,Miyuki Harada,Tetsuya Hirata,Yasushi Hirota,Kaori Koga,Katsutoshi Oda,Tomoyuki Fujii,Yutaka Osuga

Redox biology 19:166-178 PubMed30172101

2018

Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas E Buglak,Wulin Jiang,Edward S M Bahnson

Journal of applied physiology (Bethesda, Md. : 198 125:938-946 PubMed29792553

2018

Mitochondrial complex I defect resulting from exercise-induced lower limb ischemia in patients with peripheral arterial disease.

Applications

Unspecified application

Species

Unspecified reactive species

I Signolet,P Abraham,S Chupin,M Ammi,N Gueguen,F Letournel,J Picquet,C Baufreton,M Daligault,V Procaccio,P Reynier,S Henni

Frontiers in neuroscience 10:437 PubMed27729845

2016

In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Gasperini,Elisa Meneghetti,Giuseppe Legname,Federico Benetti

Histology and histopathology 23:1477-83 PubMed18830933

2008

Decreased expression of antioxidant enzymes in the conjunctival epithelium of dry eye (Sjögren's syndrome) and its possible contribution to the development of ocular surface oxidative injuries.

Applications

IHC-Fr

Species

Human

J Cejková,T Ardan,Z Simonová,C Cejka,J Malec,D Dotrelová,B Brunová

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB13499)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise