AB183881

Anti-Superoxide Dismutase 1 antibody

Name: Anti-Superoxide Dismutase 1 antibody (ab183881) | Abcam
Brand: Abcam Limited
SKU: AB183881

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(10 Publications)

Rabbit Polyclonal Superoxide Dismutase 1 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOD1 aa 50 to C-terminus.

View Alternative Names

Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1

4 Images

Supplier Data

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

SOD1 shRNA transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH, whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

15% SDS-PAGE

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution

All lanes:

Rat brain whole cell lysate at 50 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human SOD1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P00441

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.

Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information SOD1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 104:105472 PubMed40582162

2025

Melatonin protects chicken follicular granulosa cells against oxidative damage-induced apoptosis via inhibiting FOXO1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelu Liu,Keqian Di,Xiangyu Chen,Chenxuan Huang,Fei Hou,Jianku Hou,Erying Hao,Kang Bai,Dehe Wang,Lei Shi,Yifan Chen,Hui Chen

Frontiers in cell and developmental biology 13:1611226 PubMed40530334

2025

Induced neural stem cells ameliorate blood-brain barrier injury by modulating the calcium signaling pathway of astrocyte in cerebral ischemia-reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyun Liang,Chuanshang Cao,Ningmei Liu,Dongmei Chen,Ting Liu,Haibin Ma,Jiaxin Liu,Taojuan Wu,Jianguo Niu

Environment & health (Washington, D.C.) 3:58-67 PubMed39839250

2025

Unveiling the Origin of Copper Accumulation in Plasma with Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Weichao Wang,Dawei Lu,Hang Yang,Zigu Chen,Weibo Ling,Shanjun Song,Linyi Peng,Qian Liu,Guibin Jiang

Cell reports 43:114750 PubMed39283743

2024

Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ionel Sandovici,Denise S Fernandez-Twinn,Niamh Campbell,Wendy N Cooper,Yoichi Sekita,Ilona Zvetkova,David Ferland-McCollough,Haydn M Prosser,Lila M Oyama,Lucas C Pantaleão,Danilo Cimadomo,Karina Barbosa de Queiroz,Cecilia S K Cheuk,Nicola M Smith,Richard G Kay,Robin Antrobus,Katharina Hoelle,Marcella K L Ma,Noel H Smith,Stefan H Geyer,Lukas F Reissig,Wolfgang J Weninger,Kenneth Siddle,Anne E Willis,Brian Y H Lam,Martin Bushell,Susan E Ozanne,Miguel Constância

Oxidative medicine and cellular longevity 2023:6829931 PubMed37360501

2023

Improvement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models.

Applications

Unspecified application

Species

Unspecified reactive species

Nadira Chettouh-Hammas,Fabienne Fasani,Amandine Boileau,David Gosset,Giovanni Busco,Catherine Grillon

iScience 26:107022 PubMed37360683

2023

Induced neural stem cells suppressed neuroinflammation by inhibiting the microglial pyroptotic pathway in intracerebral hemorrhage rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Liu,Chuanshang Cao,Yiran Jin,Yan Wang,Xiaona Ma,Jiahui Li,Songlin Guo,Jiancheng Yang,Jianguo Niu,Xueyun Liang

Oxidative medicine and cellular longevity 2022:5236908 PubMed36471865

2022

Attenuation of the Severity of Acute Respiratory Distress Syndrome by Pomiferin through Blocking Inflammation and Oxidative Stress in an AKT/Foxo1 Pathway-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Tang,Zetian Yang,Hui Feng,Xuefeng Zhou,Ming Mao

Molecular biology reports 49:7337-7345 PubMed35585377

2022

Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Jiaxin Liu,Baocong Yu,Yiran Jin,Jiahui Li,Xiaona Ma,Jianqiang Yu,Jianguo Niu,Xueyun Liang

Experimental and therapeutic medicine 20:269 PubMed33199994

2020

Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hainian Zhu,Gang Chen,Yuhua Wang,Xuchen Lin,Jingyuan Zhou,Zengshun Wang,Nanangxiu Suo

Journal of cellular physiology 234:5203-5214 PubMed30238989

2018

Molecular mechanisms involved in the protective effect of pituitary adenylate cyclase-activating polypeptide in an in vitro model of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Grazia Maugeri,Agata G D'Amico,Daniela M Rasà,Concetta Federico,Salvatore Saccone,Giovanna Morello,Valentina La Cognata,Sebastiano Cavallaro,Velia D'Agata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Superoxide Dismutase 1 antibody

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB183881)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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