AB52950

Anti-Superoxide Dismutase 1 antibody

Name: Anti-Superoxide Dismutase 1 antibody (ab52950) | Abcam
Brand: Abcam Limited
SKU: AB52950
Rating: 5 (2 reviews)

(2 Reviews)

(28 Publications)

Rabbit Polyclonal Superoxide Dismutase 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 28 publications.

View Alternative Names

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase, Superoxide dismutase 1, hSod1, SOD1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB52950)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB52950)

ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in human placenta by Immunohistochemistry, Paraffin embedded tissue.

Unknown

Western blot - Anti-Superoxide Dismutase 1 antibody (AB52950)

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab52950) at 1/5000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Antigen retrieval is not essential but may optimise staining.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.

Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems (PubMed : 24140062). Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides (PubMed : 36630448).

See full target information SOD1

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 87:103864 PubMed40972157

2025

Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Chaudhary,Dibita Mandal,Bhagya Shree Choudhary,Sushmita Patra,Darshan Jain,Pritam Poonia,Shagufa Shaikh,Siddhi Tekalkar,Shivani Malvankar,Anusha Shivashankar,Eeshrita Jog,Leena Pilankar,Rahul Thorat,Vaishali V Kailje,Sonal Khanna,Subhakankha Manna,Bushra K Khan,Anjana Jadhav,Kedar Sharma,Soundharya Ramu,Sarthak Sahoo,Mohit Kumar Jolly,Sorab N Dalal,Sanju Sinha,Nishanth Ulhas Nair,Eytan Ruppin,Fabienne Lamballe,Flavio Maina,Nandini Verma

Acta neuropathologica communications 13:169 PubMed40783546

2025

Divergent and convergent TMEM106B pathology in murine models of neurodegeneration and human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Muzi Du,Suleyman C Akerman,Charlotte M Fare,Linhao Ruan,Svetlana Vidensky,Lyudmila Mamedova,Katie Koo,Joshua Lee,Jeffrey D Rothstein

Comprehensive Physiology 15:e70009 PubMed40176466

2025

Synergistic Effects of Riluzole and Sodium Butyrate on Barrier Function and Disease Progression of Amyotrophic Lateral Sclerosis Through the Gut-Neuron Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongguo Zhang,KaReisha Robinson,Yinglin Xia,Jun Sun

Redox biology 69:102972 PubMed38056310

2023

ALS-linked SOD1 mutations impair mitochondrial-derived vesicle formation and accelerate aging.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Guo,Teng Guan,Qiang Yu,Nitesh Sanghai,Kashfia Shafiq,Meiyu Li,Xin Jiao,Donghui Na,Guohui Zhang,Jiming Kong

Cellular and molecular life sciences : CMLS 80:304 PubMed37752364

2023

Selective removal of misfolded SOD1 delays disease onset in a mouse model of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Guan,Ting Zhou,Xiaosha Zhang,Ying Guo,Chaoxian Yang,Justin Lin,Jiasi Vicky Zhang,Yongquan Cheng,Hassan Marzban,Yu Tian Wang,Jiming Kong

American journal of physiology. Renal physiology 325:F164-F176 PubMed37318988

2023

Renal, but not platelet or skin, extracellular vesicles decrease oxidative stress, enhance nascent peptide synthesis, and protect from ischemic renal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jesus H Dominguez,Danhui Xie,K J Kelly

Oxidative medicine and cellular longevity 2022:8217663 PubMed35178161

2022

MPO/HOCl Facilitates Apoptosis and Ferroptosis in the SOD1 Motor Neuron of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jialing Peng,Jingrui Pan,Jingjing Mo,Ying Peng

The journal of obstetrics and gynaecology research 47:1817-1824 PubMed33611838

2021

The effects of recombinant klotho in cisplatin-induced ovarian failure in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ismail Biyik,Fikriye Yasemin Ozatik,Mustafa Albayrak,Orhan Ozatik,Yasemin Teksen,Neziha Senem Ari,Cenk Soysal

Applied biochemistry and biotechnology 190:634-644 PubMed31407160

2019

Studies on the Neuroprotection of Osthole on Glutamate-Induced Apoptotic Cells and an Alzheimer's Disease Mouse Model via Modulation Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qiubo Chu,Yanfeng Zhu,Tianjiao Cao,Yi Zhang,Zecheng Chang,Yan Liu,Jiahui Lu,Yizhi Zhang

Scientific reports 9:6724 PubMed31040321

2019

SOD1-positive aggregate accumulation in the CNS predicts slower disease progression and increased longevity in a mutant SOD1 mouse model of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Cindy Gill,James P Phelan,Theo Hatzipetros,Joshua D Kidd,Valerie R Tassinari,Beth Levine,Monica Z Wang,Andrew Moreno,Kenneth Thompson,Marcel Maier,Jan Grimm,Alan Gill,Fernando G Vieira

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB52950)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB52950)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise