Rabbit Recombinant Monoclonal Superoxide Dismutase 1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 49 publications.
Images
![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img182153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)")
![Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunocytochemistry-immunofluorescence-img95747.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)")
![Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunocytochemistry-immunofluorescence-img18675.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107060.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)")
![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img13227.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (AB51254)")
Publications
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- iScience 26:1082362023Exosomes derived from platelet-rich plasma promote diabetic wound healing via the JAK2/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhai Cao,Xiaotong Meng,Fangming Cao,Jinpeng Wang,Maowei YangPubMed 37953957
- Chinese medicine 18:1342023Cordycepin from Cordyceps militaris ameliorates diabetic nephropathy via the miR-193b-5p/MCL-1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Zheng,Weijie Zhang,Jufang Song,Yifei Zhong,Rong ZhuPubMed 37833817
- Neuropharmacology 241:1097302023Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet.Applications:WBReactive species:MouseClémence Viguier,Sébastien Bullich,Marlene Botella,Laure Fasseu,Amélie Alfonso,Khaoula Rekik,Sébastien Gauzin,Bruno P Guiard,Noélie DavezacPubMed 37758019
- iScience 26:1077132023Butyrate prevents visceral adipose tissue inflammation and metabolic alterations in a Friedreich's ataxia mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRiccardo Turchi,Francesca Sciarretta,Veronica Ceci,Marta Tiberi,Matteo Audano,Silvia Pedretti,Concetta Panebianco,Valentina Nesci,Valerio Pazienza,Alberto Ferri,Simone Carotti,Valerio Chiurchiù,Nico Mitro,Daniele Lettieri-Barbato,Katia AquilanoPubMed 37701569
- Antioxidants (Basel, Switzerland) 12:2023Copper(II) Complexes with Carnosine Conjugates of Hyaluronic Acids at Different Dipeptide Loading Percentages Behave as Multiple SOD Mimics and Stimulate Nrf2 Translocation and Antioxidant Response in In Vitro Inflammatory Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesco Bellia,Valeria Lanza,Irina Naletova,Barbara Tomasello,Valeria Ciaffaglione,Valentina Greco,Sebastiano Sciuto,Pietro Amico,Rosanna Inturri,Susanna Vaccaro,Tiziana Campagna,Francesco Attanasio,Giovanni Tabbì,Enrico RizzarelliPubMed 37627627
- Cancer cell international 23:1712023A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYung-Ding Bow,Ching-Chung Ko,Wen-Tsan Chang,Sih-Yan Chou,Chun-Tzu Hung,Jau-Ling Huang,Chih-Hua Tseng,Yeh-Long Chen,Ruei-Nian Li,Chien-Chih ChiuPubMed 37587444
- Chinese medicine 18:742023Panax notoginseng alleviates oxidative stress through miRNA regulations based on systems biology approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Tang,Yi-Gang Chen,Hsi-Yuan Huang,Shang-Fu Li,Hua-Li Zuo,Ji-Hang Chen,Li-Ping Li,Run-Bo Mao,Yang-Chi-Dung Lin,Hsien-Da HuangPubMed 37337262
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229852023A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Lv,Yijia Wang,Chenxiao Zhen,Junyu Liu,Xin Chen,Gejing Zhang,Wei Yao,Huijie Guo,Yunpeng Wei,Shenghang Wang,Jiancheng Yang,Peng ShangPubMed 37249350
- The Journal of physiology 601:2917-29332023High-intensity interval training in the form of isometric contraction improves fatigue resistance in dystrophin-deficient muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNao Yamauchi,Katsuyuki Tamai,Iori Kimura,Azuma Naito,Nao Tokuda,Yuki Ashida,Norio Motohashi,Yoshitsugu Aoki,Takashi YamadaPubMed 37184335
- Journal of advanced research 46:87-1002023Persistent activation of Nrf2 in a p62-dependent non-canonical manner aggravates lead-induced kidney injury by promoting apoptosis and inhibiting autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCai-Yu Lian,Bing-Xin Chu,Wei-Hao Xia,Zhen-Yong Wang,Rui-Feng Fan,Lin WangPubMed 37003700
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Tested | Expected | Tested |
| Rat | Not recommended | Tested | Tested | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50000 | Notes - |
Species Rat | Dilution info 1/50000 | Notes - |
Species Human | Dilution info 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes - |
Species Rat | Dilution info 1/100 - 1/250 | Notes - |
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1
Recommended products
Rabbit Recombinant Monoclonal Superoxide Dismutase 1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 49 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1727Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
- Biological function summary
The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.
- Pathways
SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.
- Associated diseases and disorders
Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img182153.jpg "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
ab51254 was shown to react with SOD1 in wild-type HeLa cells in Western blot with loss of signal observed in a SOD1 knockout cell line. Wild-type HeLa and SOD1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab51254 overnight at 4 °C at a 1/15000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Predicted band size: 15 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunocytochemistry-immunofluorescence-img95747.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cell line Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunocytochemistry-immunofluorescence-img18675.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107060.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img13227.jpg "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Blocking/diluting Buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 0.015 µg/mL
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 15 kDa
Observed band size: 18 kDa
![Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--flow-cytometry-intracellular-img27554.jpg "Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Superoxide Dismutase 1 with ab51254 at 1/20 dilution (1μg) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img85090.jpg "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
All lanes: Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution
All lanes: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 18 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86713.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--flow-cytometry-intracellular-img27554.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
![Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--western-blot-img85090.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254), expandable thumbnail](https://content.abcam.com/products/images/superoxide-dismutase-1-antibody-ep1727y-ab51254--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86713.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)")
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